NDUA8_BOVIN
ID NDUA8_BOVIN Reviewed; 172 AA.
AC P42029; Q2KJF6;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8;
DE AltName: Full=Complex I-19kD;
DE Short=CI-19kD;
DE AltName: Full=Complex I-PGIV;
DE Short=CI-PGIV;
DE AltName: Full=NADH-ubiquinone oxidoreductase 19 kDa subunit;
GN Name=NDUFA8;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 2-28.
RC TISSUE=Heart;
RX PubMed=1830204; DOI=10.1042/bj2770011;
RA Dupuis A., Skehel J.M., Walker J.E.;
RT "NADH:ubiquinone oxidoreductase from bovine mitochondria. cDNA sequence of
RT a 19 kDa cysteine-rich subunit.";
RL Biochem. J. 277:11-15(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Ileum;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP SUBUNIT, IDENTIFICATION IN COMPLEX I, AND SUBCELLULAR LOCATION.
RX PubMed=18721790; DOI=10.1016/j.ab.2008.07.029;
RA Lemma-Gray P., Valusova E., Carroll C.A., Weintraub S.T., Musatov A.,
RA Robinson N.C.;
RT "Subunit analysis of bovine heart complex I by reversed-phase high-
RT performance liquid chromatography, electrospray ionization-tandem mass
RT spectrometry, and matrix-assisted laser desorption/ionization-time-of-
RT flight mass spectrometry.";
RL Anal. Biochem. 382:116-121(2008).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed not to be
CC involved in catalysis. Complex I functions in the transfer of electrons
CC from NADH to the respiratory chain. The immediate electron acceptor for
CC the enzyme is believed to be ubiquinone.
CC {ECO:0000250|UniProtKB:P51970}.
CC -!- SUBUNIT: Complex I is composed of 45 different subunits.
CC {ECO:0000269|PubMed:18721790}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000305|PubMed:18721790}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:P51970}. Mitochondrion intermembrane space
CC {ECO:0000250|UniProtKB:P51970}. Mitochondrion
CC {ECO:0000250|UniProtKB:P51970}.
CC -!- DOMAIN: Contains four C-X9-C motifs that are predicted to form a helix-
CC coil-helix structure, permitting the formation of intramolecular
CC disulfide bonds. {ECO:0000250|UniProtKB:P51970}.
CC -!- SIMILARITY: Belongs to the complex I NDUFA8 subunit family.
CC {ECO:0000305}.
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DR EMBL; X59697; CAA42218.1; -; mRNA.
DR EMBL; BC105365; AAI05366.1; -; mRNA.
DR PIR; S16208; S16208.
DR RefSeq; NP_787020.1; NM_175826.4.
DR PDB; 5LC5; EM; 4.35 A; X=1-114.
DR PDB; 5LDW; EM; 4.27 A; X=1-114.
DR PDB; 5LDX; EM; 5.60 A; X=6-114.
DR PDB; 5O31; EM; 4.13 A; X=2-172.
DR PDB; 7QSD; EM; 3.10 A; X=1-172.
DR PDBsum; 5LC5; -.
DR PDBsum; 5LDW; -.
DR PDBsum; 5LDX; -.
DR PDBsum; 5O31; -.
DR PDBsum; 7QSD; -.
DR AlphaFoldDB; P42029; -.
DR SMR; P42029; -.
DR CORUM; P42029; -.
DR DIP; DIP-38811N; -.
DR IntAct; P42029; 1.
DR STRING; 9913.ENSBTAP00000005628; -.
DR TCDB; 3.D.1.6.1; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR PaxDb; P42029; -.
DR PeptideAtlas; P42029; -.
DR PRIDE; P42029; -.
DR Ensembl; ENSBTAT00000005628; ENSBTAP00000005628; ENSBTAG00000004295.
DR GeneID; 327710; -.
DR KEGG; bta:327710; -.
DR CTD; 4702; -.
DR VEuPathDB; HostDB:ENSBTAG00000004295; -.
DR VGNC; VGNC:31951; NDUFA8.
DR eggNOG; KOG3458; Eukaryota.
DR GeneTree; ENSGT00390000008938; -.
DR HOGENOM; CLU_081931_2_1_1; -.
DR InParanoid; P42029; -.
DR OMA; DRPNKEF; -.
DR OrthoDB; 1526152at2759; -.
DR TreeFam; TF105633; -.
DR Reactome; R-BTA-611105; Respiratory electron transport.
DR Reactome; R-BTA-6799198; Complex I biogenesis.
DR Proteomes; UP000009136; Chromosome 11.
DR Bgee; ENSBTAG00000004295; Expressed in cardiac atrium and 107 other tissues.
DR GO; GO:0005758; C:mitochondrial intermembrane space; IEA:UniProtKB-SubCell.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IDA:UniProtKB.
DR GO; GO:0044877; F:protein-containing complex binding; IEA:Ensembl.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; IEA:InterPro.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR InterPro; IPR010625; CHCH.
DR InterPro; IPR016680; NDUFA8.
DR PANTHER; PTHR13344; PTHR13344; 1.
DR Pfam; PF06747; CHCH; 1.
DR PIRSF; PIRSF017016; NDUA8; 1.
DR PROSITE; PS51808; CHCH; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Disulfide bond;
KW Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW Reference proteome; Repeat; Respiratory chain; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:1830204"
FT CHAIN 2..172
FT /note="NADH dehydrogenase [ubiquinone] 1 alpha subcomplex
FT subunit 8"
FT /id="PRO_0000118733"
FT DOMAIN 33..74
FT /note="CHCH 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT DOMAIN 75..118
FT /note="CHCH 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT REGION 133..159
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 36..46
FT /note="Cx9C motif 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT MOTIF 56..66
FT /note="Cx9C motif 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT MOTIF 78..88
FT /note="Cx9C motif 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT MOTIF 100..110
FT /note="Cx9C motif 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT DISULFID 36..66
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT DISULFID 46..56
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT DISULFID 78..110
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT DISULFID 88..100
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
SQ SEQUENCE 172 AA; 20091 MW; C5EEB86E73BF22AB CRC64;
MPGIVELPSL EDLKVQEVKV SSSVLKAAAH HYGAQCDKPN KEFMLCRWEE KDPRRCLEEG
KLVNQCALEF FRQIKRHCAE PFTEYWTCID YSGLQLFRRC RKQQAQFDEC VLDKLGWVRP
DLGDLSKVTK VKTDRPLPEN PYHSRARPEP NPEVEGDLKP ARHGSRLFFW TM
