NDUA8_NEUCR
ID NDUA8_NEUCR Reviewed; 183 AA.
AC P21976; Q7RVL8;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 31-AUG-2004, sequence version 2.
DT 25-MAY-2022, entry version 126.
DE RecName: Full=NADH-ubiquinone oxidoreductase 20.8 kDa subunit;
GN ORFNames=B1O14.280, NCU02472;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 38-45.
RX PubMed=2142943; DOI=10.1016/s0021-9258(19)38267-5;
RA Videira A., Tropschug M., Wachter E., Schneider H., Werner S.;
RT "Molecular cloning of subunits of complex I from Neurospora crassa. Primary
RT structure and in vitro expression of a 22-kDa polypeptide.";
RL J. Biol. Chem. 265:13060-13065(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12655011; DOI=10.1093/nar/gkg293;
RA Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT genome sequence.";
RL Nucleic Acids Res. 31:1944-1954(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed not to be
CC involved in catalysis. Complex I functions in the transfer of electrons
CC from NADH to the respiratory chain. The immediate electron acceptor for
CC the enzyme is believed to be ubiquinone.
CC -!- COFACTOR:
CC Name=iron-sulfur cluster; Xref=ChEBI:CHEBI:30408;
CC Evidence={ECO:0000305};
CC Note=Binds 1 iron-sulfur cluster. {ECO:0000305};
CC -!- SUBUNIT: Complex I is composed of about 40 different subunits. This is
CC a component of the hydrophobic fraction.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane.
CC -!- SIMILARITY: Belongs to the complex I NDUFA8 subunit family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M55323; AAA33571.1; -; mRNA.
DR EMBL; BX842631; CAE76413.1; -; Genomic_DNA.
DR EMBL; CM002236; EAA35830.1; -; Genomic_DNA.
DR PIR; A36621; A36621.
DR PIR; T47251; T47251.
DR RefSeq; XP_965066.1; XM_959973.3.
DR AlphaFoldDB; P21976; -.
DR SMR; P21976; -.
DR STRING; 5141.EFNCRP00000001979; -.
DR TCDB; 3.D.1.6.2; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR EnsemblFungi; EAA35830; EAA35830; NCU02472.
DR GeneID; 3881206; -.
DR KEGG; ncr:NCU02472; -.
DR VEuPathDB; FungiDB:NCU02472; -.
DR HOGENOM; CLU_081931_1_0_1; -.
DR InParanoid; P21976; -.
DR Proteomes; UP000001805; Chromosome 1, Linkage Group I.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IBA:GO_Central.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; IEA:InterPro.
DR InterPro; IPR010625; CHCH.
DR InterPro; IPR016680; NDUFA8.
DR PANTHER; PTHR13344; PTHR13344; 1.
DR Pfam; PF06747; CHCH; 1.
DR PIRSF; PIRSF017016; NDUA8; 1.
DR PROSITE; PS51808; CHCH; 2.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Electron transport; Membrane;
KW Mitochondrion; Mitochondrion inner membrane; Reference proteome; Repeat;
KW Respiratory chain; Transport.
FT CHAIN 1..183
FT /note="NADH-ubiquinone oxidoreductase 20.8 kDa subunit"
FT /id="PRO_0000118736"
FT DOMAIN 44..87
FT /note="CHCH 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT DOMAIN 88..130
FT /note="CHCH 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT REGION 161..183
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 47..57
FT /note="Cx9C motif 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT MOTIF 69..79
FT /note="Cx9C motif 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT MOTIF 91..101
FT /note="Cx9C motif 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT MOTIF 112..122
FT /note="Cx9C motif 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT COMPBIAS 165..183
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 47..79
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT DISULFID 57..69
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT DISULFID 91..122
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT DISULFID 101..112
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT CONFLICT 20
FT /note="P -> R (in Ref. 1; AAA33571)"
FT /evidence="ECO:0000305"
FT CONFLICT 76
FT /note="V -> L (in Ref. 1; AAA33571)"
FT /evidence="ECO:0000305"
FT CONFLICT 177
FT /note="A -> R (in Ref. 1; AAA33571)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 183 AA; 20753 MW; 45F2F8650B63AAC2 CRC64;
MASRIPQFNQ QVLYDTTPLP DSIPKVKELG ASSAPLMSAA YFIGARCRDY NDDFMQCKNE
NPGKGEFECL KEGRRVTRCA RSVIADINKS CLEEFRKHWT CLEDNNQQLW QCRPAEWKLN
KCVFENLGLK KEIPDQPPNV TPVHLRKQMI YAHWPIPRSA EPFVPPTQTG DNNKAPAAAS
SSS
