NDUA9_ARATH
ID NDUA9_ARATH Reviewed; 402 AA.
AC Q9SK66; B9DHU2; Q93VY5;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial;
DE Flags: Precursor;
GN OrderedLocusNames=At2g20360; ORFNames=F11A3.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Xiao Y.-L., Underwood B.A., Moskal W.A. Jr., Torian U., Redman J.C.,
RA Wu H.C., Utterback T., Town C.D.;
RT "Reconstruction of cDNA sequences for hypothetical genes in Arabidopsis
RT thaliana from 5' and 3' RACE products.";
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 68-402.
RC STRAIN=cv. Columbia; TISSUE=Flower, and Silique;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND CLEAVAGE OF TRANSIT PEPTIDE AFTER
RP TYR-43.
RX PubMed=25732537; DOI=10.1093/jxb/erv064;
RA Carrie C., Venne A.S., Zahedi R.P., Soll J.;
RT "Identification of cleavage sites and substrate proteins for two
RT mitochondrial intermediate peptidases in Arabidopsis thaliana.";
RL J. Exp. Bot. 66:2691-2708(2015).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed not to be
CC involved in catalysis. Complex I functions in the transfer of electrons
CC from NADH to the respiratory chain. The immediate electron acceptor for
CC the enzyme is believed to be ubiquinone (By similarity). {ECO:0000250}.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC Note=Binds 1 FAD per subunit. {ECO:0000250};
CC -!- SUBUNIT: Complex I is composed of at least 49 different subunits. This
CC a component of the hydrophobic protein fraction.
CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix
CC {ECO:0000305|PubMed:25732537}.
CC -!- SIMILARITY: Belongs to the complex I NDUFA9 subunit family.
CC {ECO:0000305}.
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DR EMBL; AC006569; AAD21752.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC06997.1; -; Genomic_DNA.
DR EMBL; AY035040; AAK59545.1; -; mRNA.
DR EMBL; AY051072; AAK93749.1; -; mRNA.
DR EMBL; AY600552; AAT68351.1; -; mRNA.
DR EMBL; AY924745; AAX23820.1; -; mRNA.
DR EMBL; AK317648; BAH20309.1; -; mRNA.
DR PIR; C84588; C84588.
DR RefSeq; NP_565469.1; NM_127595.4.
DR PDB; 7A23; EM; 3.70 A; T=1-402.
DR PDB; 7A24; EM; 3.80 A; T=1-402.
DR PDB; 7AQR; EM; 2.91 A; P=1-402.
DR PDB; 7AR7; EM; 3.72 A; P=52-382.
DR PDB; 7AR8; EM; 3.53 A; P=1-402.
DR PDB; 7ARB; EM; 3.41 A; P=1-402.
DR PDBsum; 7A23; -.
DR PDBsum; 7A24; -.
DR PDBsum; 7AQR; -.
DR PDBsum; 7AR7; -.
DR PDBsum; 7AR8; -.
DR PDBsum; 7ARB; -.
DR AlphaFoldDB; Q9SK66; -.
DR SMR; Q9SK66; -.
DR BioGRID; 1908; 10.
DR IntAct; Q9SK66; 2.
DR STRING; 3702.AT2G20360.1; -.
DR TCDB; 3.D.1.6.3; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR PaxDb; Q9SK66; -.
DR PRIDE; Q9SK66; -.
DR ProteomicsDB; 251303; -.
DR EnsemblPlants; AT2G20360.1; AT2G20360.1; AT2G20360.
DR GeneID; 816555; -.
DR Gramene; AT2G20360.1; AT2G20360.1; AT2G20360.
DR KEGG; ath:AT2G20360; -.
DR Araport; AT2G20360; -.
DR TAIR; locus:2039017; AT2G20360.
DR eggNOG; KOG2865; Eukaryota.
DR HOGENOM; CLU_007383_6_4_1; -.
DR InParanoid; Q9SK66; -.
DR OMA; FFNRFAA; -.
DR OrthoDB; 721605at2759; -.
DR PhylomeDB; Q9SK66; -.
DR BioCyc; ARA:AT2G20360-MON; -.
DR BioCyc; MetaCyc:AT2G20360-MON; -.
DR PRO; PR:Q9SK66; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SK66; baseline and differential.
DR Genevisible; Q9SK66; AT.
DR GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; HDA:TAIR.
DR GO; GO:0005739; C:mitochondrion; IDA:TAIR.
DR GO; GO:0044877; F:protein-containing complex binding; IBA:GO_Central.
DR GO; GO:1901006; P:ubiquinone-6 biosynthetic process; IBA:GO_Central.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF13460; NAD_binding_10; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Electron transport; FAD; Flavoprotein; Mitochondrion;
KW Reference proteome; Respiratory chain; Transit peptide; Transport.
FT TRANSIT 1..43
FT /note="Mitochondrion"
FT /evidence="ECO:0000269|PubMed:25732537"
FT CHAIN 44..402
FT /note="NADH dehydrogenase [ubiquinone] 1 alpha subcomplex
FT subunit 9, mitochondrial"
FT /id="PRO_0000410934"
FT STRAND 59..62
FT /evidence="ECO:0007829|PDB:7AQR"
FT STRAND 70..74
FT /evidence="ECO:0007829|PDB:7AQR"
FT TURN 75..77
FT /evidence="ECO:0007829|PDB:7AQR"
FT HELIX 81..90
FT /evidence="ECO:0007829|PDB:7AQR"
FT STRAND 94..99
FT /evidence="ECO:0007829|PDB:7AQR"
FT TURN 103..106
FT /evidence="ECO:0007829|PDB:7AQR"
FT HELIX 107..109
FT /evidence="ECO:0007829|PDB:7AQR"
FT TURN 110..112
FT /evidence="ECO:0007829|PDB:7AQR"
FT STRAND 113..122
FT /evidence="ECO:0007829|PDB:7AQR"
FT HELIX 129..133
FT /evidence="ECO:0007829|PDB:7AQR"
FT STRAND 139..143
FT /evidence="ECO:0007829|PDB:7AQR"
FT STRAND 152..154
FT /evidence="ECO:0007829|PDB:7AQR"
FT HELIX 156..160
FT /evidence="ECO:0007829|PDB:7AQR"
FT HELIX 162..173
FT /evidence="ECO:0007829|PDB:7AQR"
FT STRAND 178..182
FT /evidence="ECO:0007829|PDB:7AQR"
FT HELIX 195..210
FT /evidence="ECO:0007829|PDB:7AQR"
FT STRAND 215..219
FT /evidence="ECO:0007829|PDB:7ARB"
FT STRAND 222..224
FT /evidence="ECO:0007829|PDB:7AQR"
FT HELIX 230..240
FT /evidence="ECO:0007829|PDB:7AQR"
FT STRAND 241..245
FT /evidence="ECO:0007829|PDB:7AQR"
FT HELIX 258..269
FT /evidence="ECO:0007829|PDB:7AQR"
FT STRAND 271..273
FT /evidence="ECO:0007829|PDB:7AQR"
FT STRAND 279..282
FT /evidence="ECO:0007829|PDB:7ARB"
FT HELIX 290..301
FT /evidence="ECO:0007829|PDB:7AQR"
FT STRAND 307..309
FT /evidence="ECO:0007829|PDB:7AQR"
FT HELIX 312..323
FT /evidence="ECO:0007829|PDB:7AQR"
FT STRAND 328..331
FT /evidence="ECO:0007829|PDB:7AQR"
FT HELIX 341..346
FT /evidence="ECO:0007829|PDB:7AQR"
FT HELIX 360..363
FT /evidence="ECO:0007829|PDB:7AQR"
FT HELIX 373..380
FT /evidence="ECO:0007829|PDB:7AQR"
SQ SEQUENCE 402 AA; 43936 MW; 74073A25BCA62F12 CRC64;
MQVVSRRLVQ RPLVGGASIY SSSSLRSLYG VSNHLNGTDN CRYSSSLATK GVGHLARKGT
GGRSSVSGIV ATVFGATGFL GRYLVQQLAK MGSQVLVPFR GSEDSPRHLK LMGDLGQVVP
MKFDPRDEDS IKAVMAKANV VINLIGREYE TRNFSFEDAN HHIAEKLALV AKEHGGIMRY
IQVSCLGASV SSPSRMLRAK AAAEEAVLNA LPEATIMRPA TMIGTEDRIL NPWSMFVKKY
GFLPLIGGGT TKFQPVYVVD VAAAIVAALK DDGSSMGKTY ELGGPDVFTT HELAEIMYDM
IREWPRYVKL PFPIAKAMAA PRDFMVNKVP FPLPSPQIFN LDQINALTTD TLVSDNALKF
QDLDLVPHKL KGYPVEFLIQ YRKGGPNFGS TVSEKIPTDF YP
