NDUAC_MOUSE
ID NDUAC_MOUSE Reviewed; 145 AA.
AC Q7TMF3; Q3TIA0;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2004, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12;
DE AltName: Full=Complex I-B17.2;
DE Short=CI-B17.2;
DE Short=CIB17.2;
DE AltName: Full=NADH-ubiquinone oxidoreductase subunit B17.2;
GN Name=Ndufa12;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and DBA/2J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed not to be
CC involved in catalysis. Complex I functions in the transfer of electrons
CC from NADH to the respiratory chain. The immediate electron acceptor for
CC the enzyme is believed to be ubiquinone.
CC {ECO:0000250|UniProtKB:Q9UI09}.
CC -!- SUBUNIT: Complex I is composed of 45 different subunits.
CC {ECO:0000250|UniProtKB:Q9UI09}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:Q9UI09}; Peripheral membrane protein
CC {ECO:0000255}; Matrix side {ECO:0000250|UniProtKB:Q9UI09}.
CC -!- SIMILARITY: Belongs to the complex I NDUFA12 subunit family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB26955.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK010460; BAB26955.2; ALT_INIT; mRNA.
DR EMBL; AK167943; BAE39946.1; -; mRNA.
DR CCDS; CCDS24132.2; -.
DR RefSeq; NP_079827.2; NM_025551.3.
DR PDB; 6G2J; EM; 3.30 A; q=2-145.
DR PDB; 6G72; EM; 3.90 A; q=2-145.
DR PDB; 6ZR2; EM; 3.10 A; q=1-145.
DR PDB; 6ZTQ; EM; 3.00 A; q=1-145.
DR PDB; 7AK5; EM; 3.17 A; q=1-145.
DR PDB; 7AK6; EM; 3.82 A; q=1-145.
DR PDB; 7B93; EM; 3.04 A; q=1-145.
DR PDB; 7PSA; EM; 3.40 A; q=1-145.
DR PDBsum; 6G2J; -.
DR PDBsum; 6G72; -.
DR PDBsum; 6ZR2; -.
DR PDBsum; 6ZTQ; -.
DR PDBsum; 7AK5; -.
DR PDBsum; 7AK6; -.
DR PDBsum; 7B93; -.
DR PDBsum; 7PSA; -.
DR AlphaFoldDB; Q7TMF3; -.
DR SMR; Q7TMF3; -.
DR BioGRID; 211458; 38.
DR ComplexPortal; CPX-266; Mitochondrial respiratory chain complex I.
DR CORUM; Q7TMF3; -.
DR IntAct; Q7TMF3; 5.
DR STRING; 10090.ENSMUSP00000136313; -.
DR iPTMnet; Q7TMF3; -.
DR PhosphoSitePlus; Q7TMF3; -.
DR SwissPalm; Q7TMF3; -.
DR EPD; Q7TMF3; -.
DR jPOST; Q7TMF3; -.
DR MaxQB; Q7TMF3; -.
DR PaxDb; Q7TMF3; -.
DR PeptideAtlas; Q7TMF3; -.
DR PRIDE; Q7TMF3; -.
DR ProteomicsDB; 293537; -.
DR Antibodypedia; 30095; 179 antibodies from 27 providers.
DR DNASU; 66414; -.
DR Ensembl; ENSMUST00000020209; ENSMUSP00000020209; ENSMUSG00000020022.
DR GeneID; 66414; -.
DR KEGG; mmu:66414; -.
DR CTD; 55967; -.
DR MGI; MGI:1913664; Ndufa12.
DR VEuPathDB; HostDB:ENSMUSG00000020022; -.
DR eggNOG; KOG3382; Eukaryota.
DR GeneTree; ENSGT00390000005848; -.
DR HOGENOM; CLU_110455_1_0_1; -.
DR InParanoid; Q7TMF3; -.
DR OrthoDB; 1475919at2759; -.
DR TreeFam; TF106106; -.
DR Reactome; R-MMU-611105; Respiratory electron transport.
DR Reactome; R-MMU-6799198; Complex I biogenesis.
DR BioGRID-ORCS; 66414; 0 hits in 59 CRISPR screens.
DR ChiTaRS; Ndufa12; mouse.
DR PRO; PR:Q7TMF3; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q7TMF3; protein.
DR Bgee; ENSMUSG00000020022; Expressed in right kidney and 88 other tissues.
DR ExpressionAtlas; Q7TMF3; baseline and differential.
DR Genevisible; Q7TMF3; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISO:MGI.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0009060; P:aerobic respiration; IC:ComplexPortal.
DR GO; GO:0042775; P:mitochondrial ATP synthesis coupled electron transport; ISO:MGI.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; IEA:InterPro.
DR GO; GO:0042776; P:proton motive force-driven mitochondrial ATP synthesis; IC:ComplexPortal.
DR GO; GO:0006979; P:response to oxidative stress; ISO:MGI.
DR InterPro; IPR007763; NDUFA12.
DR PANTHER; PTHR12910; PTHR12910; 1.
DR Pfam; PF05071; NDUFA12; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Electron transport; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Respiratory chain;
KW Transport.
FT CHAIN 1..145
FT /note="NADH dehydrogenase [ubiquinone] 1 alpha subcomplex
FT subunit 12"
FT /id="PRO_0000118846"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:O97725"
FT HELIX 3..14
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 16..19
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 20..30
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 37..41
FT /evidence="ECO:0007829|PDB:7B93"
FT STRAND 43..45
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 47..50
FT /evidence="ECO:0007829|PDB:7B93"
FT STRAND 58..61
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 68..71
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 73..75
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 79..81
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 84..90
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 98..100
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 107..109
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 118..122
FT /evidence="ECO:0007829|PDB:6ZTQ"
SQ SEQUENCE 145 AA; 17086 MW; B53B37CD0DCDE8CA CRC64;
MELVEVLKRG VQQVTGHGGL RGLLRVFFRA NDIRIGTLVG EDKYGNKYYE DNKQFFGRHR
WVIYTTEMNG KNTFWDVDGS MVPPEWHRWL HCMTDDPPTT NPPTARKFIW TNHKFNVSAT
PEQYVPYSTT RKKIHEWVPP STPYK
