NDUAD_BOVIN
ID NDUAD_BOVIN Reviewed; 144 AA.
AC Q95KV7; Q3T0G4;
DT 27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13;
DE AltName: Full=Cell death regulatory protein GRIM-19;
DE AltName: Full=Complex I-B16.6;
DE Short=CI-B16.6;
DE AltName: Full=Gene associated with retinoic-interferon-induced mortality 19 protein;
DE Short=GRIM-19;
DE AltName: Full=NADH-ubiquinone oxidoreductase B16.6 subunit;
GN Name=NDUFA13; Synonyms=GRIM19;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, ACETYLATION AT ALA-2,
RP AND MASS SPECTROMETRY.
RC TISSUE=Heart;
RX PubMed=11522775; DOI=10.1074/jbc.c100444200;
RA Fearnley I.M., Carroll J., Shannon R.J., Runswick M.J., Walker J.E.,
RA Hirst J.;
RT "GRIM-19, a cell death regulatory gene product, is a subunit of bovine
RT mitochondrial NADH:ubiquinone oxidoreductase (complex I).";
RL J. Biol. Chem. 276:38345-38348(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Ileum;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP SUBUNIT, IDENTIFICATION IN COMPLEX I, AND SUBCELLULAR LOCATION.
RX PubMed=18721790; DOI=10.1016/j.ab.2008.07.029;
RA Lemma-Gray P., Valusova E., Carroll C.A., Weintraub S.T., Musatov A.,
RA Robinson N.C.;
RT "Subunit analysis of bovine heart complex I by reversed-phase high-
RT performance liquid chromatography, electrospray ionization-tandem mass
RT spectrometry, and matrix-assisted laser desorption/ionization-time-of-
RT flight mass spectrometry.";
RL Anal. Biochem. 382:116-121(2008).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed not to be
CC involved in catalysis. Complex I functions in the transfer of electrons
CC from NADH to the respiratory chain. The immediate electron acceptor for
CC the enzyme is believed to be ubiquinone. Involved in the
CC interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This
CC apoptotic activity is inhibited by interaction with viral IRF1.
CC Prevents the transactivation of STAT3 target genes. May play a role in
CC CARD15-mediated innate mucosal responses and serve to regulate
CC intestinal epithelial cell responses to microbes.
CC {ECO:0000250|UniProtKB:Q9P0J0}.
CC -!- SUBUNIT: Complex I is composed of 45 different subunits
CC (PubMed:18721790). Interacts with CARD15, but not with CARD4. Interacts
CC with STAT3, but not with STAT1, STAT2 and STAT5A. Interacts with OLFM4.
CC {ECO:0000250|UniProtKB:Q9P0J0, ECO:0000269|PubMed:18721790}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000305|PubMed:18721790}; Single-pass membrane protein
CC {ECO:0000255}; Matrix side {ECO:0000305}. Nucleus
CC {ECO:0000250|UniProtKB:Q9P0J0}. Note=Localizes mainly in the
CC mitochondrion. May be translocated into the nucleus upon IFN/RA
CC treatment. {ECO:0000250|UniProtKB:Q9P0J0}.
CC -!- MASS SPECTROMETRY: Mass=16585; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:11522775};
CC -!- SIMILARITY: Belongs to the complex I NDUFA13 subunit family.
CC {ECO:0000305}.
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DR EMBL; AJ316011; CAC87049.1; -; mRNA.
DR EMBL; BC102406; AAI02407.1; -; mRNA.
DR RefSeq; NP_788845.1; NM_176672.2.
DR PDB; 5LC5; EM; 4.35 A; Z=32-100.
DR PDB; 5LDW; EM; 4.27 A; Z=32-100.
DR PDB; 5LDX; EM; 5.60 A; Z=32-100.
DR PDB; 5LNK; EM; 3.90 A; q=2-144.
DR PDB; 5O31; EM; 4.13 A; Z=1-99.
DR PDB; 7QSD; EM; 3.10 A; Z=1-144.
DR PDBsum; 5LC5; -.
DR PDBsum; 5LDW; -.
DR PDBsum; 5LDX; -.
DR PDBsum; 5LNK; -.
DR PDBsum; 5O31; -.
DR PDBsum; 7QSD; -.
DR AlphaFoldDB; Q95KV7; -.
DR SMR; Q95KV7; -.
DR CORUM; Q95KV7; -.
DR DIP; DIP-38831N; -.
DR IntAct; Q95KV7; 2.
DR STRING; 9913.ENSBTAP00000010276; -.
DR TCDB; 3.D.1.6.1; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR iPTMnet; Q95KV7; -.
DR PaxDb; Q95KV7; -.
DR PRIDE; Q95KV7; -.
DR Ensembl; ENSBTAT00000010276; ENSBTAP00000010276; ENSBTAG00000007812.
DR GeneID; 338084; -.
DR KEGG; bta:338084; -.
DR CTD; 51079; -.
DR VEuPathDB; HostDB:ENSBTAG00000007812; -.
DR VGNC; VGNC:31946; NDUFA13.
DR eggNOG; KOG3300; Eukaryota.
DR GeneTree; ENSGT00390000000719; -.
DR HOGENOM; CLU_119720_0_0_1; -.
DR InParanoid; Q95KV7; -.
DR OMA; GFRPAAY; -.
DR OrthoDB; 1496781at2759; -.
DR TreeFam; TF315182; -.
DR Reactome; R-BTA-611105; Respiratory electron transport.
DR Reactome; R-BTA-6799198; Complex I biogenesis.
DR Proteomes; UP000009136; Chromosome 7.
DR Bgee; ENSBTAG00000007812; Expressed in laryngeal cartilage and 105 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005746; C:mitochondrial respirasome; IDA:UniProtKB.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IDA:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; ISS:UniProtKB.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IDA:UniProtKB.
DR GO; GO:0097190; P:apoptotic signaling pathway; ISS:UniProtKB.
DR GO; GO:0035458; P:cellular response to interferon-beta; IEA:Ensembl.
DR GO; GO:0071300; P:cellular response to retinoic acid; IEA:Ensembl.
DR GO; GO:0097191; P:extrinsic apoptotic signaling pathway; IEA:Ensembl.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR GO; GO:0030308; P:negative regulation of cell growth; ISS:UniProtKB.
DR GO; GO:2001243; P:negative regulation of intrinsic apoptotic signaling pathway; IEA:Ensembl.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0043280; P:positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; IEA:Ensembl.
DR GO; GO:0045732; P:positive regulation of protein catabolic process; IEA:Ensembl.
DR GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IEA:Ensembl.
DR GO; GO:0072593; P:reactive oxygen species metabolic process; IEA:Ensembl.
DR InterPro; IPR009346; GRIM-19.
DR PANTHER; PTHR12966; PTHR12966; 1.
DR Pfam; PF06212; GRIM-19; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Apoptosis; Direct protein sequencing;
KW Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW Nucleus; Reference proteome; Respiratory chain; Transmembrane;
KW Transmembrane helix; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:11522775"
FT CHAIN 2..144
FT /note="NADH dehydrogenase [ubiquinone] 1 alpha subcomplex
FT subunit 13"
FT /id="PRO_0000118803"
FT TRANSMEM 30..51
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000269|PubMed:11522775"
SQ SEQUENCE 144 AA; 16673 MW; 9AA885FFA4748347 CRC64;
MAASKVKQDM PPVGGYGPID YKRNLPRRGL SGYSMFAVGI GALLFGYWSM MKWNRERRRL
QIEDFEARIA LMPLLQAEKD RRVLQMLREN LEEEATVMKD VPGWKVGESV FHTTRWVTPM
MGELYGLRAS EEVLSATYGF IWYT
