NDUB2_HUMAN
ID NDUB2_HUMAN Reviewed; 105 AA.
AC O95178; Q6FGI6;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial;
DE AltName: Full=Complex I-AGGG;
DE Short=CI-AGGG;
DE AltName: Full=NADH-ubiquinone oxidoreductase AGGG subunit;
DE Flags: Precursor;
GN Name=NDUFB2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9878551; DOI=10.1006/bbrc.1998.9786;
RA Loeffen J.L.C.M., Triepels R.H., van den Heuvel L.P., Schuelke M.,
RA Buskens C.A.F., Smeets R.J.P., Trijbels J.M.F., Smeitink J.A.M.;
RT "cDNA of eight nuclear encoded subunits of NADH:ubiquinone oxidoreductase:
RT human complex I cDNA characterization completed.";
RL Biochem. Biophys. Res. Commun. 253:415-422(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Umbilical cord blood;
RX PubMed=11042152; DOI=10.1101/gr.140200;
RA Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G.,
RA Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W.,
RA Tao J., Huang Q.-H., Zhou J., Hu G.-X., Gu J., Chen S.-J., Chen Z.;
RT "Cloning and functional analysis of cDNAs with open reading frames for 300
RT previously undefined genes expressed in CD34+ hematopoietic stem/progenitor
RT cells.";
RL Genome Res. 10:1546-1560(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P.,
RA Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y.,
RA LaBaer J.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 34-44.
RC TISSUE=Leukemic T-cell;
RX PubMed=19892738; DOI=10.1073/pnas.0908958106;
RA Xu G., Shin S.B., Jaffrey S.R.;
RT "Global profiling of protease cleavage sites by chemoselective labeling of
RT protein N-termini.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:19310-19315(2009).
RN [6]
RP IDENTIFICATION IN THE NADH-UBIQUINONE OXIDOREDUCTASE COMPLEX,
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=12611891; DOI=10.1074/jbc.c300064200;
RA Murray J., Zhang B., Taylor S.W., Oglesbee D., Fahy E., Marusich M.F.,
RA Ghosh S.S., Capaldi R.A.;
RT "The subunit composition of the human NADH dehydrogenase obtained by rapid
RT one-step immunopurification.";
RL J. Biol. Chem. 278:13619-13622(2003).
RN [7]
RP CLEAVAGE OF TRANSIT PEPTIDE [LARGE SCALE ANALYSIS] AFTER HIS-33, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
RN [8]
RP FUNCTION, AND IDENTIFICATION IN THE NADH-UBIQUINONE OXIDOREDUCTASE COMPLEX.
RX PubMed=27626371; DOI=10.1038/nature19754;
RA Stroud D.A., Surgenor E.E., Formosa L.E., Reljic B., Frazier A.E.,
RA Dibley M.G., Osellame L.D., Stait T., Beilharz T.H., Thorburn D.R.,
RA Salim A., Ryan M.T.;
RT "Accessory subunits are integral for assembly and function of human
RT mitochondrial complex I.";
RL Nature 538:123-126(2016).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed not to be
CC involved in catalysis. Complex I functions in the transfer of electrons
CC from NADH to the respiratory chain. The immediate electron acceptor for
CC the enzyme is believed to be ubiquinone. {ECO:0000269|PubMed:27626371}.
CC -!- SUBUNIT: Complex I is composed of 45 different subunits.
CC {ECO:0000269|PubMed:12611891, ECO:0000269|PubMed:27626371}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000305|PubMed:12611891}; Peripheral membrane protein
CC {ECO:0000305}; Matrix side {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the complex I NDUFB2 subunit family.
CC {ECO:0000305}.
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DR EMBL; AF050639; AAD05428.1; -; mRNA.
DR EMBL; AF067166; AAD32450.1; -; mRNA.
DR EMBL; CR542121; CAG46918.1; -; mRNA.
DR EMBL; BC001168; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS5862.1; -.
DR PIR; JE0384; JE0384.
DR RefSeq; NP_004537.1; NM_004546.2.
DR PDB; 5XTC; EM; 3.70 A; Y=39-97.
DR PDB; 5XTD; EM; 3.70 A; Y=39-97.
DR PDB; 5XTH; EM; 3.90 A; Y=39-97.
DR PDB; 5XTI; EM; 17.40 A; BY/Y=39-97.
DR PDBsum; 5XTC; -.
DR PDBsum; 5XTD; -.
DR PDBsum; 5XTH; -.
DR PDBsum; 5XTI; -.
DR AlphaFoldDB; O95178; -.
DR SMR; O95178; -.
DR BioGRID; 110788; 36.
DR ComplexPortal; CPX-577; Mitochondrial respiratory chain complex I.
DR CORUM; O95178; -.
DR IntAct; O95178; 2.
DR STRING; 9606.ENSP00000419087; -.
DR BindingDB; O95178; -.
DR ChEMBL; CHEMBL2363065; -.
DR DrugBank; DB00157; NADH.
DR DrugCentral; O95178; -.
DR iPTMnet; O95178; -.
DR PhosphoSitePlus; O95178; -.
DR BioMuta; NDUFB2; -.
DR EPD; O95178; -.
DR jPOST; O95178; -.
DR MassIVE; O95178; -.
DR MaxQB; O95178; -.
DR PaxDb; O95178; -.
DR PeptideAtlas; O95178; -.
DR PRIDE; O95178; -.
DR ProteomicsDB; 50689; -.
DR TopDownProteomics; O95178; -.
DR Antibodypedia; 46193; 60 antibodies from 21 providers.
DR DNASU; 4708; -.
DR Ensembl; ENST00000247866.9; ENSP00000247866.4; ENSG00000090266.13.
DR Ensembl; ENST00000465506.5; ENSP00000419357.1; ENSG00000090266.13.
DR Ensembl; ENST00000476279.5; ENSP00000419087.1; ENSG00000090266.13.
DR GeneID; 4708; -.
DR KEGG; hsa:4708; -.
DR MANE-Select; ENST00000247866.9; ENSP00000247866.4; NM_004546.3; NP_004537.1.
DR UCSC; uc003vwa.4; human.
DR CTD; 4708; -.
DR DisGeNET; 4708; -.
DR GeneCards; NDUFB2; -.
DR HGNC; HGNC:7697; NDUFB2.
DR HPA; ENSG00000090266; Low tissue specificity.
DR MIM; 603838; gene.
DR neXtProt; NX_O95178; -.
DR OpenTargets; ENSG00000090266; -.
DR PharmGKB; PA31503; -.
DR VEuPathDB; HostDB:ENSG00000090266; -.
DR eggNOG; ENOG502S524; Eukaryota.
DR GeneTree; ENSGT00390000004044; -.
DR HOGENOM; CLU_177133_1_0_1; -.
DR InParanoid; O95178; -.
DR OMA; WPDASKW; -.
DR OrthoDB; 1541745at2759; -.
DR PhylomeDB; O95178; -.
DR TreeFam; TF316619; -.
DR BioCyc; MetaCyc:HS01679-MON; -.
DR PathwayCommons; O95178; -.
DR Reactome; R-HSA-611105; Respiratory electron transport.
DR Reactome; R-HSA-6799198; Complex I biogenesis.
DR SignaLink; O95178; -.
DR SIGNOR; O95178; -.
DR BioGRID-ORCS; 4708; 169 hits in 1085 CRISPR screens.
DR ChiTaRS; NDUFB2; human.
DR GeneWiki; NDUFB2; -.
DR GenomeRNAi; 4708; -.
DR Pharos; O95178; Tclin.
DR PRO; PR:O95178; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; O95178; protein.
DR Bgee; ENSG00000090266; Expressed in apex of heart and 202 other tissues.
DR ExpressionAtlas; O95178; baseline and differential.
DR Genevisible; O95178; HS.
DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:ComplexPortal.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IDA:UniProtKB.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; NAS:UniProtKB.
DR GO; GO:0009060; P:aerobic respiration; IC:ComplexPortal.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; NAS:UniProtKB.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; IMP:UniProtKB.
DR GO; GO:0042776; P:proton motive force-driven mitochondrial ATP synthesis; IC:ComplexPortal.
DR InterPro; IPR026627; NDUFB2_animal.
DR PANTHER; PTHR15223; PTHR15223; 1.
DR Pfam; PF14813; NADH_B2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Electron transport; Membrane;
KW Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Respiratory chain; Transit peptide; Transport.
FT TRANSIT 1..33
FT /note="Mitochondrion"
FT /evidence="ECO:0000269|PubMed:19892738,
FT ECO:0007744|PubMed:25944712"
FT CHAIN 34..105
FT /note="NADH dehydrogenase [ubiquinone] 1 beta subcomplex
FT subunit 2, mitochondrial"
FT /id="PRO_0000020042"
FT REGION 85..105
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 105 AA; 12058 MW; 5698368608CDC16F CRC64;
MSALTRLASF ARVGGRLFRS GCARTAGDGG VRHAGGGVHI EPRYRQFPQL TRSQVFQSEF
FSGLMWFWIL WRFWHDSEEV LGHFPYPDPS QWTDEELGIP PDDED
