NDUB2_MOUSE
ID NDUB2_MOUSE Reviewed; 105 AA.
AC Q9CPU2;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial;
DE AltName: Full=Complex I-AGGG;
DE Short=CI-AGGG;
DE AltName: Full=NADH-ubiquinone oxidoreductase AGGG subunit;
DE Flags: Precursor;
GN Name=Ndufb2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, Embryonic stem cell, and Pancreas;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, and Pancreas;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed not to be
CC involved in catalysis. Complex I functions in the transfer of electrons
CC from NADH to the respiratory chain. The immediate electron acceptor for
CC the enzyme is believed to be ubiquinone.
CC {ECO:0000250|UniProtKB:O95178}.
CC -!- SUBUNIT: Complex I is composed of 45 different subunits.
CC {ECO:0000250|UniProtKB:O95178}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:O95178}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:O95178}; Matrix side
CC {ECO:0000250|UniProtKB:O95178}.
CC -!- SIMILARITY: Belongs to the complex I NDUFB2 subunit family.
CC {ECO:0000305}.
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DR EMBL; AK007495; BAB25068.1; -; mRNA.
DR EMBL; AK010642; BAB27084.1; -; mRNA.
DR EMBL; AK019008; BAB31508.1; -; mRNA.
DR EMBL; AK028334; BAC25887.1; -; mRNA.
DR EMBL; BC013510; AAH13510.1; -; mRNA.
DR CCDS; CCDS20025.1; -.
DR RefSeq; NP_080888.1; NM_026612.3.
DR RefSeq; XP_006506592.1; XM_006506529.3.
DR PDB; 6G2J; EM; 3.30 A; j=1-105.
DR PDB; 6G72; EM; 3.90 A; j=1-105.
DR PDB; 6ZR2; EM; 3.10 A; j=1-105.
DR PDB; 6ZTQ; EM; 3.00 A; j=1-105.
DR PDB; 7AK5; EM; 3.17 A; j=1-105.
DR PDB; 7AK6; EM; 3.82 A; j=1-105.
DR PDB; 7B93; EM; 3.04 A; j=1-105.
DR PDB; 7PSA; EM; 3.40 A; j=1-105.
DR PDBsum; 6G2J; -.
DR PDBsum; 6G72; -.
DR PDBsum; 6ZR2; -.
DR PDBsum; 6ZTQ; -.
DR PDBsum; 7AK5; -.
DR PDBsum; 7AK6; -.
DR PDBsum; 7B93; -.
DR PDBsum; 7PSA; -.
DR AlphaFoldDB; Q9CPU2; -.
DR SMR; Q9CPU2; -.
DR ComplexPortal; CPX-266; Mitochondrial respiratory chain complex I.
DR CORUM; Q9CPU2; -.
DR IntAct; Q9CPU2; 1.
DR STRING; 10090.ENSMUSP00000114095; -.
DR PhosphoSitePlus; Q9CPU2; -.
DR jPOST; Q9CPU2; -.
DR MaxQB; Q9CPU2; -.
DR PaxDb; Q9CPU2; -.
DR PeptideAtlas; Q9CPU2; -.
DR PRIDE; Q9CPU2; -.
DR ProteomicsDB; 252797; -.
DR TopDownProteomics; Q9CPU2; -.
DR Antibodypedia; 46193; 60 antibodies from 21 providers.
DR DNASU; 68198; -.
DR Ensembl; ENSMUST00000119379; ENSMUSP00000114095; ENSMUSG00000002416.
DR Ensembl; ENSMUST00000135671; ENSMUSP00000118918; ENSMUSG00000002416.
DR GeneID; 68198; -.
DR KEGG; mmu:68198; -.
DR UCSC; uc009bmc.1; mouse.
DR CTD; 4708; -.
DR MGI; MGI:1915448; Ndufb2.
DR VEuPathDB; HostDB:ENSMUSG00000002416; -.
DR eggNOG; ENOG502S524; Eukaryota.
DR GeneTree; ENSGT00390000004044; -.
DR HOGENOM; CLU_177133_1_0_1; -.
DR InParanoid; Q9CPU2; -.
DR OMA; WPDASKW; -.
DR OrthoDB; 1541745at2759; -.
DR PhylomeDB; Q9CPU2; -.
DR TreeFam; TF316619; -.
DR Reactome; R-MMU-611105; Respiratory electron transport.
DR Reactome; R-MMU-6799198; Complex I biogenesis.
DR BioGRID-ORCS; 68198; 17 hits in 71 CRISPR screens.
DR ChiTaRS; Ndufb2; mouse.
DR PRO; PR:Q9CPU2; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q9CPU2; protein.
DR Bgee; ENSMUSG00000002416; Expressed in interventricular septum and 267 other tissues.
DR Genevisible; Q9CPU2; MM.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISO:MGI.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0009060; P:aerobic respiration; IC:ComplexPortal.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR GO; GO:0042776; P:proton motive force-driven mitochondrial ATP synthesis; IC:ComplexPortal.
DR InterPro; IPR026627; NDUFB2_animal.
DR PANTHER; PTHR15223; PTHR15223; 1.
DR Pfam; PF14813; NADH_B2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Electron transport; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Respiratory chain;
KW Transit peptide; Transport.
FT TRANSIT 1..33
FT /note="Mitochondrion"
FT /evidence="ECO:0000250"
FT CHAIN 34..105
FT /note="NADH dehydrogenase [ubiquinone] 1 beta subcomplex
FT subunit 2, mitochondrial"
FT /id="PRO_0000020043"
FT REGION 86..105
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT STRAND 43..46
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 52..75
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 78..80
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 89..91
FT /evidence="ECO:0007829|PDB:6ZR2"
FT TURN 95..98
FT /evidence="ECO:0007829|PDB:6ZTQ"
SQ SEQUENCE 105 AA; 11967 MW; F5D5019BFA33E510 CRC64;
MSALTRLVPF GRVGGRLLRG CRARAAGDSG VRHAGGGVHI QPRYREFPQL TRSQVIQGEF
LSSLMWFWIL WRFWHDSDAV LGHFSYPDPS QWTDEELGIP PDDED
