NDUB3_BOVIN
ID NDUB3_BOVIN Reviewed; 98 AA.
AC Q02365; Q2KJB0;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3;
DE AltName: Full=Complex I-B12;
DE Short=CI-B12;
DE AltName: Full=NADH-ubiquinone oxidoreductase B12 subunit;
GN Name=NDUFB3;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC TISSUE=Heart;
RX PubMed=1518044; DOI=10.1016/0022-2836(92)91052-q;
RA Walker J.E., Arizmendi J.M., Dupuis A., Fearnley I.M., Finel M., Medd S.M.,
RA Pilkington S.J., Runswick M.J., Skehel J.M.;
RT "Sequences of 20 subunits of NADH:ubiquinone oxidoreductase from bovine
RT heart mitochondria. Application of a novel strategy for sequencing proteins
RT using the polymerase chain reaction.";
RL J. Mol. Biol. 226:1051-1072(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Kidney;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP PROTEIN SEQUENCE OF 1-13, ACETYLATION AT ALA-2, METHYLATION AT HIS-5; HIS-7
RP AND HIS-9, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=15728260; DOI=10.1074/mcp.m500014-mcp200;
RA Carroll J., Fearnley I.M., Skehel J.M., Runswick M.J., Shannon R.J.,
RA Hirst J., Walker J.E.;
RT "The post-translational modifications of the nuclear encoded subunits of
RT complex I from bovine heart mitochondria.";
RL Mol. Cell. Proteomics 4:693-699(2005).
RN [4]
RP SUBUNIT, IDENTIFICATION IN COMPLEX I, AND SUBCELLULAR LOCATION.
RX PubMed=18721790; DOI=10.1016/j.ab.2008.07.029;
RA Lemma-Gray P., Valusova E., Carroll C.A., Weintraub S.T., Musatov A.,
RA Robinson N.C.;
RT "Subunit analysis of bovine heart complex I by reversed-phase high-
RT performance liquid chromatography, electrospray ionization-tandem mass
RT spectrometry, and matrix-assisted laser desorption/ionization-time-of-
RT flight mass spectrometry.";
RL Anal. Biochem. 382:116-121(2008).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed not to be
CC involved in catalysis. Complex I functions in the transfer of electrons
CC from NADH to the respiratory chain. The immediate electron acceptor for
CC the enzyme is believed to be ubiquinone.
CC {ECO:0000250|UniProtKB:O43676}.
CC -!- SUBUNIT: Complex I is composed of 45 different subunits.
CC {ECO:0000269|PubMed:18721790}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000305|PubMed:18721790}; Single-pass membrane protein
CC {ECO:0000305}; Matrix side {ECO:0000305}.
CC -!- PTM: Methylation at His residues by METTL9 enhances complex I-mediated
CC mitochondrial respiration. {ECO:0000250|UniProtKB:O43676}.
CC -!- SIMILARITY: Belongs to the complex I NDUFB3 subunit family.
CC {ECO:0000305}.
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DR EMBL; X63213; CAA44898.1; -; mRNA.
DR EMBL; BC105433; AAI05434.1; -; mRNA.
DR PIR; S28243; S28243.
DR RefSeq; NP_788840.1; NM_176667.2.
DR PDB; 7QSD; EM; 3.10 A; k=1-98.
DR PDBsum; 7QSD; -.
DR AlphaFoldDB; Q02365; -.
DR SMR; Q02365; -.
DR CORUM; Q02365; -.
DR DIP; DIP-38800N; -.
DR IntAct; Q02365; 3.
DR STRING; 9913.ENSBTAP00000016957; -.
DR TCDB; 3.D.1.6.1; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR iPTMnet; Q02365; -.
DR PaxDb; Q02365; -.
DR PRIDE; Q02365; -.
DR Ensembl; ENSBTAT00000016957; ENSBTAP00000016957; ENSBTAG00000012760.
DR GeneID; 338073; -.
DR KEGG; bta:338073; -.
DR CTD; 4709; -.
DR VEuPathDB; HostDB:ENSBTAG00000012760; -.
DR VGNC; VGNC:31963; NDUFB3.
DR eggNOG; KOG4631; Eukaryota.
DR GeneTree; ENSGT00390000010316; -.
DR HOGENOM; CLU_160226_1_0_1; -.
DR InParanoid; Q02365; -.
DR OMA; EAWRYEP; -.
DR OrthoDB; 1568057at2759; -.
DR TreeFam; TF319656; -.
DR Reactome; R-BTA-611105; Respiratory electron transport.
DR Reactome; R-BTA-6799198; Complex I biogenesis.
DR Proteomes; UP000009136; Chromosome 2.
DR Bgee; ENSBTAG00000012760; Expressed in tongue muscle and 104 other tissues.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IDA:UniProtKB.
DR GO; GO:0022900; P:electron transport chain; IEA:InterPro.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR InterPro; IPR012576; NDUFB3.
DR PANTHER; PTHR15082; PTHR15082; 1.
DR Pfam; PF08122; NDUF_B12; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Direct protein sequencing; Electron transport;
KW Membrane; Methylation; Mitochondrion; Mitochondrion inner membrane;
KW Reference proteome; Respiratory chain; Transmembrane; Transmembrane helix;
KW Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:15728260"
FT CHAIN 2..98
FT /note="NADH dehydrogenase [ubiquinone] 1 beta subcomplex
FT subunit 3"
FT /id="PRO_0000118796"
FT TRANSMEM 61..83
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylalanine; partial"
FT /evidence="ECO:0000269|PubMed:15728260"
FT MOD_RES 5
FT /note="Pros-methylhistidine"
FT /evidence="ECO:0000305|PubMed:15728260"
FT MOD_RES 7
FT /note="Pros-methylhistidine"
FT /evidence="ECO:0000305|PubMed:15728260"
FT MOD_RES 9
FT /note="Pros-methylhistidine"
FT /evidence="ECO:0000305|PubMed:15728260"
FT MOD_RES 23
FT /note="N6-acetyllysine; alternate"
FT /evidence="ECO:0000250|UniProtKB:Q9CQZ6"
FT MOD_RES 23
FT /note="N6-succinyllysine; alternate"
FT /evidence="ECO:0000250|UniProtKB:Q9CQZ6"
SQ SEQUENCE 98 AA; 11141 MW; F1251DCAB266FFD7 CRC64;
MAHGHGHEHG PSKMELPDYK QWKIEGTPLE TVQEKLAARG LRDPWGRNEA WRYMGGFANN
VSFVGALLKG FKWGFAAFVV AIGAEYYLES QKKDKKHH
