NDUB4_BOVIN
ID NDUB4_BOVIN Reviewed; 129 AA.
AC P48305; Q54A31;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4;
DE AltName: Full=Complex I-B15;
DE Short=CI-B15;
DE AltName: Full=NADH-ubiquinone oxidoreductase B15 subunit;
GN Name=NDUFB4;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, AND ACETYLATION AT
RP SER-2.
RC TISSUE=Heart;
RX PubMed=1518044; DOI=10.1016/0022-2836(92)91052-q;
RA Walker J.E., Arizmendi J.M., Dupuis A., Fearnley I.M., Finel M., Medd S.M.,
RA Pilkington S.J., Runswick M.J., Skehel J.M.;
RT "Sequences of 20 subunits of NADH:ubiquinone oxidoreductase from bovine
RT heart mitochondria. Application of a novel strategy for sequencing proteins
RT using the polymerase chain reaction.";
RL J. Mol. Biol. 226:1051-1072(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Rumen;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP PARTIAL PROTEIN SEQUENCE, SUBUNIT, IDENTIFICATION IN COMPLEX I, AND
RP SUBCELLULAR LOCATION.
RX PubMed=10852722; DOI=10.1021/bi000335t;
RA Sazanov L.A., Peak-Chew S.Y., Fearnley I.M., Walker J.E.;
RT "Resolution of the membrane domain of bovine complex I into subcomplexes:
RT implications for the structural organization of the enzyme.";
RL Biochemistry 39:7229-7235(2000).
RN [4]
RP SUBUNIT, IDENTIFICATION IN COMPLEX I, AND SUBCELLULAR LOCATION.
RX PubMed=18721790; DOI=10.1016/j.ab.2008.07.029;
RA Lemma-Gray P., Valusova E., Carroll C.A., Weintraub S.T., Musatov A.,
RA Robinson N.C.;
RT "Subunit analysis of bovine heart complex I by reversed-phase high-
RT performance liquid chromatography, electrospray ionization-tandem mass
RT spectrometry, and matrix-assisted laser desorption/ionization-time-of-
RT flight mass spectrometry.";
RL Anal. Biochem. 382:116-121(2008).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed not to be
CC involved in catalysis. Complex I functions in the transfer of electrons
CC from NADH to the respiratory chain. The immediate electron acceptor for
CC the enzyme is believed to be ubiquinone.
CC {ECO:0000250|UniProtKB:O95168}.
CC -!- SUBUNIT: Complex I is composed of 45 different subunits.
CC {ECO:0000269|PubMed:10852722, ECO:0000269|PubMed:1518044,
CC ECO:0000269|PubMed:18721790}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000305|PubMed:10852722, ECO:0000305|PubMed:18721790}; Single-pass
CC membrane protein {ECO:0000255}; Matrix side {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the complex I NDUFB4 subunit family.
CC {ECO:0000305}.
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DR EMBL; X64898; CAA46107.1; -; mRNA.
DR EMBL; BC103187; AAI03188.1; -; mRNA.
DR PIR; S28237; S28237.
DR RefSeq; NP_787016.1; NM_175822.2.
DR PDB; 5LC5; EM; 4.35 A; m=33-129.
DR PDB; 5LDW; EM; 4.27 A; m=33-129.
DR PDB; 5LDX; EM; 5.60 A; m=33-129.
DR PDB; 5O31; EM; 4.13 A; m=2-129.
DR PDB; 7QSD; EM; 3.10 A; m=1-129.
DR PDBsum; 5LC5; -.
DR PDBsum; 5LDW; -.
DR PDBsum; 5LDX; -.
DR PDBsum; 5O31; -.
DR PDBsum; 7QSD; -.
DR AlphaFoldDB; P48305; -.
DR SMR; P48305; -.
DR CORUM; P48305; -.
DR DIP; DIP-38793N; -.
DR IntAct; P48305; 2.
DR STRING; 9913.ENSBTAP00000021123; -.
DR TCDB; 3.D.1.6.1; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR iPTMnet; P48305; -.
DR PaxDb; P48305; -.
DR PRIDE; P48305; -.
DR Ensembl; ENSBTAT00000021123; ENSBTAP00000021123; ENSBTAG00000015892.
DR GeneID; 327706; -.
DR KEGG; bta:327706; -.
DR CTD; 4710; -.
DR VEuPathDB; HostDB:ENSBTAG00000015892; -.
DR eggNOG; ENOG502S2HF; Eukaryota.
DR GeneTree; ENSGT00390000007133; -.
DR InParanoid; P48305; -.
DR OMA; RWNHARH; -.
DR OrthoDB; 1434967at2759; -.
DR Proteomes; UP000009136; Chromosome 1.
DR Bgee; ENSBTAG00000015892; Expressed in oocyte and 104 other tissues.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IDA:UniProtKB.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR GO; GO:0006979; P:response to oxidative stress; IDA:UniProtKB.
DR InterPro; IPR009866; NADH_UbQ_OxRdtase_NDUFB4_su.
DR PANTHER; PTHR15469; PTHR15469; 1.
DR Pfam; PF07225; NDUF_B4; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Direct protein sequencing; Electron transport;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Phosphoprotein;
KW Reference proteome; Respiratory chain; Transmembrane; Transmembrane helix;
KW Transport.
FT INIT_MET 1
FT /note="Removed"
FT CHAIN 2..129
FT /note="NADH dehydrogenase [ubiquinone] 1 beta subcomplex
FT subunit 4"
FT /id="PRO_0000118799"
FT TRANSMEM 86..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0000269|PubMed:1518044"
FT MOD_RES 26
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O95168"
SQ SEQUENCE 129 AA; 15184 MW; F5DA3419E80D9125 CRC64;
MSFPKYEASR LSSLPTTLDP AEYDISSETR KAQAERLAIR SRLKREYQLQ YYDPSRRGVI
EDPALVRWTY ARSANIYPNF RPNTKTSLLG ALFGIGPLVF WYYVFKTDRD RKEKLIQEGK
LDRTFNISY
