NDUB4_MOUSE
ID NDUB4_MOUSE Reviewed; 129 AA.
AC Q9CQC7;
DT 27-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4;
DE AltName: Full=Complex I-B15;
DE Short=CI-B15;
DE AltName: Full=NADH-ubiquinone oxidoreductase B15 subunit;
GN Name=Ndufb4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Kidney, and Tongue;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PROTEIN SEQUENCE OF 6-40; 46-67 AND 73-85, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RA Lubec G., Kang S.U.;
RL Submitted (APR-2007) to UniProtKB.
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed not to be
CC involved in catalysis. Complex I functions in the transfer of electrons
CC from NADH to the respiratory chain. The immediate electron acceptor for
CC the enzyme is believed to be ubiquinone.
CC {ECO:0000250|UniProtKB:O95168}.
CC -!- SUBUNIT: Complex I is composed of 45 different subunits.
CC {ECO:0000250|UniProtKB:O95168}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:O95168}; Single-pass membrane protein
CC {ECO:0000255}; Matrix side {ECO:0000250|UniProtKB:O95168}.
CC -!- SIMILARITY: Belongs to the complex I NDUFB4 subunit family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK009807; BAB26515.1; -; mRNA.
DR EMBL; AK018707; BAB31358.1; -; mRNA.
DR EMBL; BC056229; AAH56229.1; -; mRNA.
DR CCDS; CCDS28161.1; -.
DR RefSeq; NP_080886.1; NM_026610.1.
DR RefSeq; XP_001476621.1; XM_001476571.4.
DR RefSeq; XP_001478493.1; XM_001478443.6.
DR PDB; 6G2J; EM; 3.30 A; m=1-129.
DR PDB; 6G72; EM; 3.90 A; m=1-129.
DR PDB; 6ZR2; EM; 3.10 A; m=1-129.
DR PDB; 6ZTQ; EM; 3.00 A; m=1-129.
DR PDB; 7AK5; EM; 3.17 A; m=1-129.
DR PDB; 7AK6; EM; 3.82 A; m=1-129.
DR PDB; 7B93; EM; 3.04 A; m=1-129.
DR PDB; 7PSA; EM; 3.40 A; m=1-129.
DR PDBsum; 6G2J; -.
DR PDBsum; 6G72; -.
DR PDBsum; 6ZR2; -.
DR PDBsum; 6ZTQ; -.
DR PDBsum; 7AK5; -.
DR PDBsum; 7AK6; -.
DR PDBsum; 7B93; -.
DR PDBsum; 7PSA; -.
DR AlphaFoldDB; Q9CQC7; -.
DR SMR; Q9CQC7; -.
DR BioGRID; 212719; 28.
DR BioGRID; 784605; 1.
DR BioGRID; 785760; 3.
DR ComplexPortal; CPX-266; Mitochondrial respiratory chain complex I.
DR CORUM; Q9CQC7; -.
DR IntAct; Q9CQC7; 8.
DR MINT; Q9CQC7; -.
DR STRING; 10090.ENSMUSP00000023514; -.
DR iPTMnet; Q9CQC7; -.
DR PhosphoSitePlus; Q9CQC7; -.
DR SwissPalm; Q9CQC7; -.
DR EPD; Q9CQC7; -.
DR jPOST; Q9CQC7; -.
DR MaxQB; Q9CQC7; -.
DR PaxDb; Q9CQC7; -.
DR PRIDE; Q9CQC7; -.
DR ProteomicsDB; 286166; -.
DR Antibodypedia; 32798; 82 antibodies from 24 providers.
DR DNASU; 68194; -.
DR Ensembl; ENSMUST00000023514; ENSMUSP00000023514; ENSMUSG00000022820.
DR GeneID; 100042503; -.
DR GeneID; 68194; -.
DR KEGG; mmu:100042503; -.
DR KEGG; mmu:68194; -.
DR UCSC; uc007zei.1; mouse.
DR CTD; 100042503; -.
DR CTD; 4710; -.
DR MGI; MGI:1915444; Ndufb4.
DR VEuPathDB; HostDB:ENSMUSG00000022820; -.
DR eggNOG; ENOG502S2HF; Eukaryota.
DR GeneTree; ENSGT00390000007133; -.
DR HOGENOM; CLU_123402_0_0_1; -.
DR InParanoid; Q9CQC7; -.
DR OMA; PLFFWFY; -.
DR OrthoDB; 20156at2759; -.
DR PhylomeDB; Q9CQC7; -.
DR TreeFam; TF328761; -.
DR Reactome; R-MMU-611105; Respiratory electron transport.
DR Reactome; R-MMU-6799198; Complex I biogenesis.
DR BioGRID-ORCS; 68194; 23 hits in 75 CRISPR screens.
DR ChiTaRS; Ndufb4; mouse.
DR PRO; PR:Q9CQC7; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q9CQC7; protein.
DR Bgee; ENSMUSG00000022820; Expressed in right kidney and 86 other tissues.
DR Genevisible; Q9CQC7; MM.
DR GO; GO:0005743; C:mitochondrial inner membrane; HDA:MGI.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0009060; P:aerobic respiration; IC:ComplexPortal.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR GO; GO:0042776; P:proton motive force-driven mitochondrial ATP synthesis; IC:ComplexPortal.
DR GO; GO:0006979; P:response to oxidative stress; ISO:MGI.
DR InterPro; IPR009866; NADH_UbQ_OxRdtase_NDUFB4_su.
DR PANTHER; PTHR15469; PTHR15469; 1.
DR Pfam; PF07225; NDUF_B4; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Direct protein sequencing; Electron transport;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Phosphoprotein;
KW Reference proteome; Respiratory chain; Transmembrane; Transmembrane helix;
KW Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:P48305"
FT CHAIN 2..129
FT /note="NADH dehydrogenase [ubiquinone] 1 beta subcomplex
FT subunit 4"
FT /id="PRO_0000118801"
FT TRANSMEM 88..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0000250|UniProtKB:P48305"
FT MOD_RES 26
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT STRAND 10..12
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 16..18
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 20..22
FT /evidence="ECO:0007829|PDB:7B93"
FT HELIX 27..51
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 63..73
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 74..76
FT /evidence="ECO:0007829|PDB:6G2J"
FT TURN 77..79
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 84..86
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 87..117
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 119..121
FT /evidence="ECO:0007829|PDB:6G2J"
SQ SEQUENCE 129 AA; 15081 MW; 9E76B5087F095062 CRC64;
MSGSKYKPAP LATLPSTLDP AEYDVSPETR RAQVERLSIR ARLKREYLLQ YNDPKRVSHI
EDPALIRWTY ARSANIYPNF RPTPKNSLLG AVAGFGPLIF WYYVFKTDRD RKERLIQEGK
LDRKFNISY
