ID   NDUB5_BOVIN             Reviewed;         189 AA.
AC   Q02380; Q3SZV4; Q56K08;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial;
DE   AltName: Full=Complex I-SGDH;
DE            Short=CI-SGDH;
DE   AltName: Full=NADH-ubiquinone oxidoreductase SGDH subunit;
DE   Flags: Precursor;
GN   Name=NDUFB5;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC   TISSUE=Heart;
RX   PubMed=1518044; DOI=10.1016/0022-2836(92)91052-q;
RA   Walker J.E., Arizmendi J.M., Dupuis A., Fearnley I.M., Finel M., Medd S.M.,
RA   Pilkington S.J., Runswick M.J., Skehel J.M.;
RT   "Sequences of 20 subunits of NADH:ubiquinone oxidoreductase from bovine
RT   heart mitochondria. Application of a novel strategy for sequencing proteins
RT   using the polymerase chain reaction.";
RL   J. Mol. Biol. 226:1051-1072(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Lymphoid epithelium;
RA   Yu J., Meng Y., Wang Z., Hansen C., Li C., Moore S.S.;
RT   "Analysis of sequences obtained from constructed full-length bovine cDNA
RT   libraries.";
RL   Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Testis;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   PARTIAL PROTEIN SEQUENCE, SUBUNIT, IDENTIFICATION IN COMPLEX I, AND
RP   SUBCELLULAR LOCATION.
RX   PubMed=10852722; DOI=10.1021/bi000335t;
RA   Sazanov L.A., Peak-Chew S.Y., Fearnley I.M., Walker J.E.;
RT   "Resolution of the membrane domain of bovine complex I into subcomplexes:
RT   implications for the structural organization of the enzyme.";
RL   Biochemistry 39:7229-7235(2000).
RN   [5]
RP   SUBUNIT, IDENTIFICATION IN COMPLEX I, AND SUBCELLULAR LOCATION.
RX   PubMed=18721790; DOI=10.1016/j.ab.2008.07.029;
RA   Lemma-Gray P., Valusova E., Carroll C.A., Weintraub S.T., Musatov A.,
RA   Robinson N.C.;
RT   "Subunit analysis of bovine heart complex I by reversed-phase high-
RT   performance liquid chromatography, electrospray ionization-tandem mass
RT   spectrometry, and matrix-assisted laser desorption/ionization-time-of-
RT   flight mass spectrometry.";
RL   Anal. Biochem. 382:116-121(2008).
CC   -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC       chain NADH dehydrogenase (Complex I), that is believed not to be
CC       involved in catalysis. Complex I functions in the transfer of electrons
CC       from NADH to the respiratory chain. The immediate electron acceptor for
CC       the enzyme is believed to be ubiquinone.
CC       {ECO:0000250|UniProtKB:O43674}.
CC   -!- SUBUNIT: Complex I is composed of 45 different subunits.
CC       {ECO:0000269|PubMed:10852722, ECO:0000269|PubMed:1518044,
CC       ECO:0000269|PubMed:18721790}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000305|PubMed:10852722, ECO:0000305|PubMed:18721790}; Single-pass
CC       membrane protein {ECO:0000255}; Matrix side {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the complex I NDUFB5 subunit family.
CC       {ECO:0000305}.
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DR   EMBL; X63208; CAA44893.1; -; mRNA.
DR   EMBL; AY911319; AAW82087.1; -; mRNA.
DR   EMBL; BC102690; AAI02691.1; -; mRNA.
DR   PIR; S28254; S28254.
DR   RefSeq; NP_788829.1; NM_176656.4.
DR   PDB; 5LC5; EM; 4.35 A; h=1-91.
DR   PDB; 5LDW; EM; 4.27 A; h=53-91.
DR   PDB; 5LDX; EM; 5.60 A; h=53-91.
DR   PDB; 5O31; EM; 4.13 A; h=47-189.
DR   PDB; 7QSD; EM; 3.10 A; h=1-189.
DR   PDBsum; 5LC5; -.
DR   PDBsum; 5LDW; -.
DR   PDBsum; 5LDX; -.
DR   PDBsum; 5O31; -.
DR   PDBsum; 7QSD; -.
DR   AlphaFoldDB; Q02380; -.
DR   SMR; Q02380; -.
DR   CORUM; Q02380; -.
DR   DIP; DIP-38825N; -.
DR   IntAct; Q02380; 2.
DR   STRING; 9913.ENSBTAP00000003197; -.
DR   TCDB; 3.D.1.6.1; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR   PaxDb; Q02380; -.
DR   PRIDE; Q02380; -.
DR   Ensembl; ENSBTAT00000003197; ENSBTAP00000003197; ENSBTAG00000002463.
DR   GeneID; 338061; -.
DR   KEGG; bta:338061; -.
DR   CTD; 4711; -.
DR   VEuPathDB; HostDB:ENSBTAG00000002463; -.
DR   VGNC; VGNC:31964; NDUFB5.
DR   eggNOG; KOG4632; Eukaryota.
DR   GeneTree; ENSGT00390000009980; -.
DR   HOGENOM; CLU_100260_2_0_1; -.
DR   InParanoid; Q02380; -.
DR   OMA; DYQAYYY; -.
DR   OrthoDB; 1251699at2759; -.
DR   TreeFam; TF313997; -.
DR   Reactome; R-BTA-611105; Respiratory electron transport.
DR   Reactome; R-BTA-6799198; Complex I biogenesis.
DR   Proteomes; UP000009136; Chromosome 1.
DR   Bgee; ENSBTAG00000002463; Expressed in tongue muscle and 104 other tissues.
DR   GO; GO:0005747; C:mitochondrial respiratory chain complex I; IDA:UniProtKB.
DR   GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR   GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR   InterPro; IPR019173; NADH_UbQ_OxRdtase_B5_su.
DR   PANTHER; PTHR13178; PTHR13178; 1.
DR   Pfam; PF09781; NDUF_B5; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Electron transport; Membrane;
KW   Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW   Respiratory chain; Transit peptide; Transmembrane; Transmembrane helix;
KW   Transport.
FT   TRANSIT         1..46
FT                   /note="Mitochondrion"
FT   CHAIN           47..189
FT                   /note="NADH dehydrogenase [ubiquinone] 1 beta subcomplex
FT                   subunit 5, mitochondrial"
FT                   /id="PRO_0000020051"
FT   TRANSMEM        73..93
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        72
FT                   /note="R -> T (in Ref. 2; AAW82087)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   189 AA;  21589 MW;  AE3ADBA0E26DEC8D CRC64;
     MAAMSLLHRA SVSAVAALSG RRLGTRLGFG GFLTRDFPKT VAPVRHSGDH GKRLFIIKPS
     GFYDKRFLKL LRFYILLTGI PVAIGITLIN VFIGEAELAE IPEGYVPEHW EYFKHPISRW
     IARTFFDGPE KNYERTMAIL QIEAEKAELR LKELEVRRLM RARGDGPWFH YPTIDKELID
     HSPKATPDN