NDUB5_HUMAN
ID NDUB5_HUMAN Reviewed; 189 AA.
AC O43674; Q561V6;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 175.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial;
DE AltName: Full=Complex I-SGDH;
DE Short=CI-SGDH;
DE AltName: Full=NADH-ubiquinone oxidoreductase SGDH subunit;
DE Flags: Precursor;
GN Name=NDUFB5;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Heart;
RX PubMed=9425316; DOI=10.1006/bbrc.1997.7707;
RA Ton C., Hwang D.M., Dempsey A.A., Liew C.-C.;
RT "Identification and primary structure of five human NADH-ubiquinone
RT oxidoreductase subunits.";
RL Biochem. Biophys. Res. Commun. 241:589-594(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16641997; DOI=10.1038/nature04728;
RA Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J.,
RA Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P.,
RA Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A.,
RA Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G.,
RA Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W.,
RA Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M.,
RA Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P.,
RA Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H.,
RA Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J.,
RA Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W.,
RA Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B.,
RA Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O.,
RA Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B.,
RA Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H.,
RA Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J.,
RA Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X.,
RA Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R.,
RA Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.;
RT "The DNA sequence, annotation and analysis of human chromosome 3.";
RL Nature 440:1194-1198(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain, Skin, and Urinary bladder;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION IN THE NADH-UBIQUINONE OXIDOREDUCTASE COMPLEX,
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=12611891; DOI=10.1074/jbc.c300064200;
RA Murray J., Zhang B., Taylor S.W., Oglesbee D., Fahy E., Marusich M.F.,
RA Ghosh S.S., Capaldi R.A.;
RT "The subunit composition of the human NADH dehydrogenase obtained by rapid
RT one-step immunopurification.";
RL J. Biol. Chem. 278:13619-13622(2003).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
RN [9]
RP FUNCTION, AND IDENTIFICATION IN THE NADH-UBIQUINONE OXIDOREDUCTASE COMPLEX.
RX PubMed=27626371; DOI=10.1038/nature19754;
RA Stroud D.A., Surgenor E.E., Formosa L.E., Reljic B., Frazier A.E.,
RA Dibley M.G., Osellame L.D., Stait T., Beilharz T.H., Thorburn D.R.,
RA Salim A., Ryan M.T.;
RT "Accessory subunits are integral for assembly and function of human
RT mitochondrial complex I.";
RL Nature 538:123-126(2016).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed not to be
CC involved in catalysis. Complex I functions in the transfer of electrons
CC from NADH to the respiratory chain. The immediate electron acceptor for
CC the enzyme is believed to be ubiquinone. {ECO:0000269|PubMed:27626371}.
CC -!- SUBUNIT: Complex I is composed of 45 different subunits.
CC {ECO:0000269|PubMed:12611891, ECO:0000269|PubMed:27626371}.
CC -!- INTERACTION:
CC O43674; P57086: SCAND1; NbExp=3; IntAct=EBI-1246293, EBI-745846;
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000305|PubMed:12611891}; Single-pass membrane protein
CC {ECO:0000305}; Matrix side {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O43674-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O43674-2; Sequence=VSP_042516;
CC -!- SIMILARITY: Belongs to the complex I NDUFB5 subunit family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF047181; AAC04266.1; -; mRNA.
DR EMBL; AC090425; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471052; EAW78393.1; -; Genomic_DNA.
DR EMBL; BC005271; AAH05271.1; -; mRNA.
DR EMBL; BC093071; AAH93071.1; -; mRNA.
DR EMBL; BC009796; AAH09796.1; -; mRNA.
DR CCDS; CCDS3234.1; -. [O43674-1]
DR CCDS; CCDS56297.1; -. [O43674-2]
DR PIR; JC5820; JC5820.
DR RefSeq; NP_001186886.1; NM_001199957.1. [O43674-2]
DR RefSeq; NP_001186887.1; NM_001199958.1.
DR RefSeq; NP_002483.1; NM_002492.3. [O43674-1]
DR PDB; 5XTC; EM; 3.70 A; a=52-189.
DR PDB; 5XTD; EM; 3.70 A; a=52-189.
DR PDB; 5XTH; EM; 3.90 A; a=52-189.
DR PDB; 5XTI; EM; 17.40 A; Ba/a=52-189.
DR PDBsum; 5XTC; -.
DR PDBsum; 5XTD; -.
DR PDBsum; 5XTH; -.
DR PDBsum; 5XTI; -.
DR AlphaFoldDB; O43674; -.
DR SMR; O43674; -.
DR BioGRID; 110791; 117.
DR ComplexPortal; CPX-577; Mitochondrial respiratory chain complex I.
DR CORUM; O43674; -.
DR IntAct; O43674; 55.
DR MINT; O43674; -.
DR STRING; 9606.ENSP00000259037; -.
DR BindingDB; O43674; -.
DR ChEMBL; CHEMBL2363065; -.
DR DrugBank; DB00157; NADH.
DR DrugCentral; O43674; -.
DR GlyGen; O43674; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; O43674; -.
DR PhosphoSitePlus; O43674; -.
DR BioMuta; NDUFB5; -.
DR EPD; O43674; -.
DR jPOST; O43674; -.
DR MassIVE; O43674; -.
DR MaxQB; O43674; -.
DR PaxDb; O43674; -.
DR PeptideAtlas; O43674; -.
DR PRIDE; O43674; -.
DR ProteomicsDB; 49102; -. [O43674-1]
DR ProteomicsDB; 49103; -. [O43674-2]
DR TopDownProteomics; O43674-1; -. [O43674-1]
DR TopDownProteomics; O43674-2; -. [O43674-2]
DR Antibodypedia; 1272; 148 antibodies from 31 providers.
DR DNASU; 4711; -.
DR Ensembl; ENST00000259037.8; ENSP00000259037.3; ENSG00000136521.13. [O43674-1]
DR Ensembl; ENST00000493866.5; ENSP00000419656.1; ENSG00000136521.13. [O43674-2]
DR GeneID; 4711; -.
DR KEGG; hsa:4711; -.
DR MANE-Select; ENST00000259037.8; ENSP00000259037.3; NM_002492.4; NP_002483.1.
DR UCSC; uc003fkc.4; human. [O43674-1]
DR CTD; 4711; -.
DR DisGeNET; 4711; -.
DR GeneCards; NDUFB5; -.
DR HGNC; HGNC:7700; NDUFB5.
DR HPA; ENSG00000136521; Tissue enhanced (skeletal).
DR MIM; 603841; gene.
DR neXtProt; NX_O43674; -.
DR OpenTargets; ENSG00000136521; -.
DR PharmGKB; PA31511; -.
DR VEuPathDB; HostDB:ENSG00000136521; -.
DR eggNOG; KOG4632; Eukaryota.
DR GeneTree; ENSGT00390000009980; -.
DR HOGENOM; CLU_100260_2_0_1; -.
DR InParanoid; O43674; -.
DR OMA; DYQAYYY; -.
DR OrthoDB; 1251699at2759; -.
DR PhylomeDB; O43674; -.
DR TreeFam; TF313997; -.
DR BioCyc; MetaCyc:HS06175-MON; -.
DR PathwayCommons; O43674; -.
DR Reactome; R-HSA-611105; Respiratory electron transport.
DR Reactome; R-HSA-6799198; Complex I biogenesis.
DR SignaLink; O43674; -.
DR SIGNOR; O43674; -.
DR BioGRID-ORCS; 4711; 134 hits in 1088 CRISPR screens.
DR ChiTaRS; NDUFB5; human.
DR GenomeRNAi; 4711; -.
DR Pharos; O43674; Tclin.
DR PRO; PR:O43674; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; O43674; protein.
DR Bgee; ENSG00000136521; Expressed in heart right ventricle and 206 other tissues.
DR ExpressionAtlas; O43674; baseline and differential.
DR Genevisible; O43674; HS.
DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:ComplexPortal.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IDA:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; IDA:HPA.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; TAS:ProtInc.
DR GO; GO:0009060; P:aerobic respiration; IC:ComplexPortal.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; TAS:ProtInc.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; IMP:UniProtKB.
DR GO; GO:0042776; P:proton motive force-driven mitochondrial ATP synthesis; IC:ComplexPortal.
DR InterPro; IPR019173; NADH_UbQ_OxRdtase_B5_su.
DR PANTHER; PTHR13178; PTHR13178; 1.
DR Pfam; PF09781; NDUF_B5; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Electron transport; Membrane;
KW Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Respiratory chain; Transit peptide; Transmembrane; Transmembrane helix;
KW Transport.
FT TRANSIT 1..46
FT /note="Mitochondrion"
FT /evidence="ECO:0000250"
FT CHAIN 47..189
FT /note="NADH dehydrogenase [ubiquinone] 1 beta subcomplex
FT subunit 5, mitochondrial"
FT /id="PRO_0000020052"
FT TRANSMEM 73..93
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 42..93
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_042516"
FT VARIANT 133
FT /note="Y -> H (in dbSNP:rs4147793)"
FT /id="VAR_050590"
SQ SEQUENCE 189 AA; 21750 MW; 66D288F7AB7FD5C5 CRC64;
MAAMSLLRRV SVTAVAALSG RPLGTRLGFG GFLTRGFPKA AAPVRHSGDH GKRLFVIRPS
RFYDRRFLKL LRFYIALTGI PVAIFITLVN VFIGQAELAE IPEGYVPEHW EYYKHPISRW
IARNFYDSPE KIYERTMAVL QIEAEKAELR VKELEVRKLM HVRGDGPWYY YETIDKELID
HSPKATPDN
