NDUB5_MOUSE
ID NDUB5_MOUSE Reviewed; 189 AA.
AC Q9CQH3;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial;
DE AltName: Full=Complex I-SGDH;
DE Short=CI-SGDH;
DE AltName: Full=NADH-ubiquinone oxidoreductase SGDH subunit;
DE Flags: Precursor;
GN Name=Ndufb5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Colon, and Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PROTEIN SEQUENCE OF 54-66; 124-131; 136-150 AND 161-176, AND IDENTIFICATION
RP BY MASS SPECTROMETRY.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RA Lubec G., Kang S.U.;
RL Submitted (APR-2007) to UniProtKB.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed not to be
CC involved in catalysis. Complex I functions in the transfer of electrons
CC from NADH to the respiratory chain. The immediate electron acceptor for
CC the enzyme is believed to be ubiquinone.
CC {ECO:0000250|UniProtKB:O43674}.
CC -!- SUBUNIT: Complex I is composed of 45 different subunits.
CC {ECO:0000250|UniProtKB:O43674}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:O43674}; Single-pass membrane protein
CC {ECO:0000250|UniProtKB:O43674}; Matrix side
CC {ECO:0000250|UniProtKB:O43674}.
CC -!- SIMILARITY: Belongs to the complex I NDUFB5 subunit family.
CC {ECO:0000305}.
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DR EMBL; AK018562; BAB31277.1; -; mRNA.
DR EMBL; AK004372; BAB23279.1; -; mRNA.
DR EMBL; AK013223; BAB28724.1; -; mRNA.
DR EMBL; BC025155; AAH25155.1; -; mRNA.
DR CCDS; CCDS17300.1; -.
DR RefSeq; NP_079592.2; NM_025316.2.
DR PDB; 6G2J; EM; 3.30 A; h=1-189.
DR PDB; 6G72; EM; 3.90 A; h=1-189.
DR PDB; 6ZR2; EM; 3.10 A; h=1-189.
DR PDB; 6ZTQ; EM; 3.00 A; h=1-189.
DR PDB; 7AK5; EM; 3.17 A; h=1-189.
DR PDB; 7AK6; EM; 3.82 A; h=1-189.
DR PDB; 7B93; EM; 3.04 A; h=1-189.
DR PDB; 7PSA; EM; 3.40 A; h=1-189.
DR PDBsum; 6G2J; -.
DR PDBsum; 6G72; -.
DR PDBsum; 6ZR2; -.
DR PDBsum; 6ZTQ; -.
DR PDBsum; 7AK5; -.
DR PDBsum; 7AK6; -.
DR PDBsum; 7B93; -.
DR PDBsum; 7PSA; -.
DR AlphaFoldDB; Q9CQH3; -.
DR SMR; Q9CQH3; -.
DR BioGRID; 211174; 36.
DR ComplexPortal; CPX-266; Mitochondrial respiratory chain complex I.
DR CORUM; Q9CQH3; -.
DR IntAct; Q9CQH3; 4.
DR STRING; 10090.ENSMUSP00000114963; -.
DR iPTMnet; Q9CQH3; -.
DR PhosphoSitePlus; Q9CQH3; -.
DR EPD; Q9CQH3; -.
DR jPOST; Q9CQH3; -.
DR MaxQB; Q9CQH3; -.
DR PaxDb; Q9CQH3; -.
DR PeptideAtlas; Q9CQH3; -.
DR PRIDE; Q9CQH3; -.
DR ProteomicsDB; 252867; -.
DR DNASU; 66046; -.
DR Ensembl; ENSMUST00000127477; ENSMUSP00000114963; ENSMUSG00000027673.
DR GeneID; 66046; -.
DR KEGG; mmu:66046; -.
DR UCSC; uc008owq.1; mouse.
DR CTD; 4711; -.
DR MGI; MGI:1913296; Ndufb5.
DR VEuPathDB; HostDB:ENSMUSG00000027673; -.
DR eggNOG; KOG4632; Eukaryota.
DR GeneTree; ENSGT00390000009980; -.
DR HOGENOM; CLU_100260_2_0_1; -.
DR InParanoid; Q9CQH3; -.
DR OMA; DYQAYYY; -.
DR OrthoDB; 1251699at2759; -.
DR PhylomeDB; Q9CQH3; -.
DR TreeFam; TF313997; -.
DR Reactome; R-MMU-611105; Respiratory electron transport.
DR Reactome; R-MMU-6799198; Complex I biogenesis.
DR BioGRID-ORCS; 66046; 18 hits in 74 CRISPR screens.
DR ChiTaRS; Ndufb5; mouse.
DR PRO; PR:Q9CQH3; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q9CQH3; protein.
DR Bgee; ENSMUSG00000027673; Expressed in plantaris and 257 other tissues.
DR ExpressionAtlas; Q9CQH3; baseline and differential.
DR Genevisible; Q9CQH3; MM.
DR GO; GO:0005743; C:mitochondrial inner membrane; HDA:MGI.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0009060; P:aerobic respiration; IC:ComplexPortal.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR GO; GO:0042776; P:proton motive force-driven mitochondrial ATP synthesis; IC:ComplexPortal.
DR InterPro; IPR019173; NADH_UbQ_OxRdtase_B5_su.
DR PANTHER; PTHR13178; PTHR13178; 1.
DR Pfam; PF09781; NDUF_B5; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Electron transport; Membrane;
KW Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Respiratory chain; Transit peptide; Transmembrane; Transmembrane helix;
KW Transport.
FT TRANSIT 1..46
FT /note="Mitochondrion"
FT /evidence="ECO:0000250"
FT CHAIN 47..189
FT /note="NADH dehydrogenase [ubiquinone] 1 beta subcomplex
FT subunit 5, mitochondrial"
FT /id="PRO_0000020053"
FT TRANSMEM 73..93
FT /note="Helical"
FT /evidence="ECO:0000255"
FT HELIX 63..92
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 109..112
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 116..125
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 129..162
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 166..169
FT /evidence="ECO:0007829|PDB:7B93"
FT HELIX 176..178
FT /evidence="ECO:0007829|PDB:7AK5"
FT STRAND 185..187
FT /evidence="ECO:0007829|PDB:6ZTQ"
SQ SEQUENCE 189 AA; 21710 MW; 8D998EA6CD683DE3 CRC64;
MAAMSLLQRA SVSALTALSC RRAGPRLGVG SFLTRSFPKT VAPVRHSGDH GKRLFVVKPS
LYYDARFLRL MKFYLMLTGI PVIIGITLVN IFIGEAELAE IPEGYIPEHW EYYKHPISRW
IARNFYDGPE KNYEKTLAIL QIESEKAELR LKEQEVRRLM RARGDGPWYQ FPTPEKEFID
HSPKATPDN
