NDUB6_MOUSE
ID NDUB6_MOUSE Reviewed; 128 AA.
AC Q3UIU2;
DT 02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6;
DE AltName: Full=Complex I-B17;
DE Short=CI-B17;
DE AltName: Full=NADH-ubiquinone oxidoreductase B17 subunit;
GN Name=Ndufb6; Synonyms=Gm137;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Amnion;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [3]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-24, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=23576753; DOI=10.1073/pnas.1302961110;
RA Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B.,
RA Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.;
RT "Label-free quantitative proteomics of the lysine acetylome in mitochondria
RT identifies substrates of SIRT3 in metabolic pathways.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed not to be
CC involved in catalysis. Complex I functions in the transfer of electrons
CC from NADH to the respiratory chain. The immediate electron acceptor for
CC the enzyme is believed to be ubiquinone.
CC {ECO:0000250|UniProtKB:O95139}.
CC -!- SUBUNIT: Complex I is composed of 45 different subunits.
CC {ECO:0000250|UniProtKB:O95139}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:O95139}; Single-pass membrane protein
CC {ECO:0000255}; Matrix side {ECO:0000250|UniProtKB:O95139}.
CC -!- SIMILARITY: Belongs to the complex I NDUFB6 subunit family.
CC {ECO:0000305}.
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DR EMBL; AK146762; BAE27414.1; -; mRNA.
DR CCDS; CCDS18045.1; -.
DR RefSeq; NP_001028477.1; NM_001033305.3.
DR PDB; 6G2J; EM; 3.30 A; i=1-128.
DR PDB; 6G72; EM; 3.90 A; i=1-128.
DR PDB; 6ZR2; EM; 3.10 A; i=1-128.
DR PDB; 6ZTQ; EM; 3.00 A; i=1-128.
DR PDB; 7AK5; EM; 3.17 A; i=2-128.
DR PDB; 7AK6; EM; 3.82 A; i=1-128.
DR PDB; 7B93; EM; 3.04 A; i=1-128.
DR PDB; 7PSA; EM; 3.40 A; i=1-128.
DR PDBsum; 6G2J; -.
DR PDBsum; 6G72; -.
DR PDBsum; 6ZR2; -.
DR PDBsum; 6ZTQ; -.
DR PDBsum; 7AK5; -.
DR PDBsum; 7AK6; -.
DR PDBsum; 7B93; -.
DR PDBsum; 7PSA; -.
DR AlphaFoldDB; Q3UIU2; -.
DR SMR; Q3UIU2; -.
DR BioGRID; 230927; 45.
DR ComplexPortal; CPX-266; Mitochondrial respiratory chain complex I.
DR CORUM; Q3UIU2; -.
DR IntAct; Q3UIU2; 4.
DR MINT; Q3UIU2; -.
DR STRING; 10090.ENSMUSP00000092746; -.
DR iPTMnet; Q3UIU2; -.
DR PhosphoSitePlus; Q3UIU2; -.
DR jPOST; Q3UIU2; -.
DR MaxQB; Q3UIU2; -.
DR PaxDb; Q3UIU2; -.
DR PRIDE; Q3UIU2; -.
DR ProteomicsDB; 252941; -.
DR Antibodypedia; 25071; 105 antibodies from 22 providers.
DR Ensembl; ENSMUST00000095128; ENSMUSP00000092746; ENSMUSG00000071014.
DR GeneID; 230075; -.
DR KEGG; mmu:230075; -.
DR UCSC; uc008shk.1; mouse.
DR CTD; 4712; -.
DR MGI; MGI:2684983; Ndufb6.
DR VEuPathDB; HostDB:ENSMUSG00000071014; -.
DR eggNOG; KOG4633; Eukaryota.
DR GeneTree; ENSGT00390000007535; -.
DR InParanoid; Q3UIU2; -.
DR OMA; KYHVNTK; -.
DR OrthoDB; 1545413at2759; -.
DR PhylomeDB; Q3UIU2; -.
DR TreeFam; TF328587; -.
DR Reactome; R-MMU-611105; Respiratory electron transport.
DR Reactome; R-MMU-6799198; Complex I biogenesis.
DR BioGRID-ORCS; 230075; 16 hits in 72 CRISPR screens.
DR ChiTaRS; Ndufb6; mouse.
DR PRO; PR:Q3UIU2; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q3UIU2; protein.
DR Bgee; ENSMUSG00000071014; Expressed in intercostal muscle and 264 other tissues.
DR ExpressionAtlas; Q3UIU2; baseline and differential.
DR Genevisible; Q3UIU2; MM.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISO:MGI.
DR GO; GO:0031966; C:mitochondrial membrane; ISO:MGI.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IDA:MGI.
DR GO; GO:0005739; C:mitochondrion; IDA:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0009060; P:aerobic respiration; IC:ComplexPortal.
DR GO; GO:0042775; P:mitochondrial ATP synthesis coupled electron transport; ISO:MGI.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; IEA:InterPro.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR GO; GO:0042776; P:proton motive force-driven mitochondrial ATP synthesis; IC:ComplexPortal.
DR InterPro; IPR019174; NADH_DH_b-subcmplx_su6.
DR PANTHER; PTHR15083; PTHR15083; 1.
DR Pfam; PF09782; NDUF_B6; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Electron transport; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Respiratory chain;
KW Transmembrane; Transmembrane helix; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q02367"
FT CHAIN 2..128
FT /note="NADH dehydrogenase [ubiquinone] 1 beta subcomplex
FT subunit 6"
FT /id="PRO_0000233980"
FT TRANSMEM 64..86
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0000250|UniProtKB:Q02367"
FT MOD_RES 24
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:23576753"
FT HELIX 6..25
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 69..74
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 76..88
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 91..93
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 96..98
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 111..113
FT /evidence="ECO:0007829|PDB:6ZTQ"
SQ SEQUENCE 128 AA; 15515 MW; C6E28FD41E455873 CRC64;
MSGYTPDEKL RLQQLRELRR RWLKDQELSP REPVLPPRRM WPLERFWDNF LRDGAVWKNM
VFKAYRSSLF AVSHVLIPMW FVHYYVKYHM ATKPYTIVSS KPRIFPGDTI LETGEVIPPM
RDFPDQHH
