NDUB7_MOUSE
ID NDUB7_MOUSE Reviewed; 137 AA.
AC Q9CR61;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7;
DE AltName: Full=Complex I-B18;
DE Short=CI-B18;
DE AltName: Full=NADH-ubiquinone oxidoreductase B18 subunit;
GN Name=Ndufb7;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Kidney;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PROTEIN SEQUENCE OF 8-35; 38-65; 72-97 AND 122-137, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RA Lubec G., Kang S.U.;
RL Submitted (APR-2007) to UniProtKB.
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-73, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed not to be
CC involved in catalysis. Complex I functions in the transfer of electrons
CC from NADH to the respiratory chain. The immediate electron acceptor for
CC the enzyme is believed to be ubiquinone.
CC {ECO:0000250|UniProtKB:P17568}.
CC -!- SUBUNIT: Complex I is composed of 45 different subunits.
CC {ECO:0000250|UniProtKB:P17568}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P17568}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:P17568}. Mitochondrion intermembrane space
CC {ECO:0000250|UniProtKB:P17568}.
CC -!- DOMAIN: Contains two C-X9-C motifs that are predicted to form a helix-
CC coil-helix structure, permitting the formation of intramolecular
CC disulfide bonds. {ECO:0000250|UniProtKB:P17568}.
CC -!- SIMILARITY: Belongs to the complex I NDUFB7 subunit family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK003300; BAB22701.1; -; mRNA.
DR EMBL; AK002678; BAB22280.1; -; mRNA.
DR EMBL; BC034787; AAH34787.1; -; mRNA.
DR CCDS; CCDS22455.1; -.
DR RefSeq; NP_080119.1; NM_025843.3.
DR PDB; 6G2J; EM; 3.30 A; o=1-137.
DR PDB; 6G72; EM; 3.90 A; o=1-137.
DR PDB; 6ZR2; EM; 3.10 A; o=1-137.
DR PDB; 6ZTQ; EM; 3.00 A; o=1-137.
DR PDB; 7AK5; EM; 3.17 A; o=1-137.
DR PDB; 7AK6; EM; 3.82 A; o=1-137.
DR PDB; 7B93; EM; 3.04 A; o=1-137.
DR PDB; 7PSA; EM; 3.40 A; o=1-137.
DR PDBsum; 6G2J; -.
DR PDBsum; 6G72; -.
DR PDBsum; 6ZR2; -.
DR PDBsum; 6ZTQ; -.
DR PDBsum; 7AK5; -.
DR PDBsum; 7AK6; -.
DR PDBsum; 7B93; -.
DR PDBsum; 7PSA; -.
DR AlphaFoldDB; Q9CR61; -.
DR SMR; Q9CR61; -.
DR BioGRID; 211808; 60.
DR ComplexPortal; CPX-266; Mitochondrial respiratory chain complex I.
DR CORUM; Q9CR61; -.
DR IntAct; Q9CR61; 2.
DR STRING; 10090.ENSMUSP00000037341; -.
DR iPTMnet; Q9CR61; -.
DR PhosphoSitePlus; Q9CR61; -.
DR EPD; Q9CR61; -.
DR jPOST; Q9CR61; -.
DR MaxQB; Q9CR61; -.
DR PaxDb; Q9CR61; -.
DR PeptideAtlas; Q9CR61; -.
DR PRIDE; Q9CR61; -.
DR ProteomicsDB; 252942; -.
DR Antibodypedia; 1259; 154 antibodies from 29 providers.
DR DNASU; 66916; -.
DR Ensembl; ENSMUST00000036996; ENSMUSP00000037341; ENSMUSG00000033938.
DR GeneID; 66916; -.
DR KEGG; mmu:66916; -.
DR UCSC; uc009mkk.1; mouse.
DR CTD; 4713; -.
DR MGI; MGI:1914166; Ndufb7.
DR VEuPathDB; HostDB:ENSMUSG00000033938; -.
DR eggNOG; KOG3468; Eukaryota.
DR GeneTree; ENSGT00390000018759; -.
DR HOGENOM; CLU_154847_1_0_1; -.
DR InParanoid; Q9CR61; -.
DR OMA; FVYQCAH; -.
DR OrthoDB; 1570033at2759; -.
DR PhylomeDB; Q9CR61; -.
DR TreeFam; TF315152; -.
DR Reactome; R-MMU-611105; Respiratory electron transport.
DR Reactome; R-MMU-6799198; Complex I biogenesis.
DR BioGRID-ORCS; 66916; 17 hits in 74 CRISPR screens.
DR ChiTaRS; Ndufb7; mouse.
DR PRO; PR:Q9CR61; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q9CR61; protein.
DR Bgee; ENSMUSG00000033938; Expressed in aortic valve and 264 other tissues.
DR Genevisible; Q9CR61; MM.
DR GO; GO:0005743; C:mitochondrial inner membrane; HDA:MGI.
DR GO; GO:0005758; C:mitochondrial intermembrane space; ISO:MGI.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0009060; P:aerobic respiration; IC:ComplexPortal.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR GO; GO:0042776; P:proton motive force-driven mitochondrial ATP synthesis; IC:ComplexPortal.
DR InterPro; IPR008698; NDUB7.
DR PANTHER; PTHR20900; PTHR20900; 1.
DR Pfam; PF05676; NDUF_B7; 1.
DR PROSITE; PS51808; CHCH; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Disulfide bond;
KW Electron transport; Lipoprotein; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Myristate; Phosphoprotein;
KW Reference proteome; Respiratory chain; Transport.
FT INIT_MET 1
FT /note="Removed"
FT CHAIN 2..137
FT /note="NADH dehydrogenase [ubiquinone] 1 beta subcomplex
FT subunit 7"
FT /id="PRO_0000118812"
FT DOMAIN 56..98
FT /note="CHCH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT MOTIF 59..69
FT /note="Cx9C motif 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT MOTIF 80..90
FT /note="Cx9C motif 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT MOD_RES 73
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000250"
FT DISULFID 59..90
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT DISULFID 69..80
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT HELIX 6..9
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 13..15
FT /evidence="ECO:0007829|PDB:7B93"
FT STRAND 19..21
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 27..30
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 43..48
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 53..55
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 60..72
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 82..112
FT /evidence="ECO:0007829|PDB:6ZTQ"
SQ SEQUENCE 137 AA; 16331 MW; 1B83033240133FE9 CRC64;
MGAHLTRRYL WDASVEPDPE KIPSFPPDLG FPERKERVMV ATQQEMMDAQ LTLQQRDYCA
HYLIRLLKCK RDSFPNFLAC KHEQHDWDYC EHLDYVKRMK EFERERRLLQ RKKRRALKEA
RVAQGQGEGE VGPEVAL
