NDUB8_BOVIN
ID NDUB8_BOVIN Reviewed; 186 AA.
AC Q02372; Q3SYR9; Q56K09;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial;
DE AltName: Full=Complex I-ASHI;
DE Short=CI-ASHI;
DE AltName: Full=NADH-ubiquinone oxidoreductase ASHI subunit;
DE Flags: Precursor;
GN Name=NDUFB8;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC TISSUE=Heart;
RX PubMed=1518044; DOI=10.1016/0022-2836(92)91052-q;
RA Walker J.E., Arizmendi J.M., Dupuis A., Fearnley I.M., Finel M., Medd S.M.,
RA Pilkington S.J., Runswick M.J., Skehel J.M.;
RT "Sequences of 20 subunits of NADH:ubiquinone oxidoreductase from bovine
RT heart mitochondria. Application of a novel strategy for sequencing proteins
RT using the polymerase chain reaction.";
RL J. Mol. Biol. 226:1051-1072(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lymphoid epithelium;
RA Yu J., Meng Y., Wang Z., Hansen C., Li C., Moore S.S.;
RT "Analysis of sequences obtained from constructed full-length bovine cDNA
RT libraries.";
RL Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Liver;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP PARTIAL PROTEIN SEQUENCE, SUBUNIT, IDENTIFICATION IN COMPLEX I, AND
RP SUBCELLULAR LOCATION.
RX PubMed=10852722; DOI=10.1021/bi000335t;
RA Sazanov L.A., Peak-Chew S.Y., Fearnley I.M., Walker J.E.;
RT "Resolution of the membrane domain of bovine complex I into subcomplexes:
RT implications for the structural organization of the enzyme.";
RL Biochemistry 39:7229-7235(2000).
RN [5]
RP SUBUNIT, IDENTIFICATION IN COMPLEX I, AND SUBCELLULAR LOCATION.
RX PubMed=18721790; DOI=10.1016/j.ab.2008.07.029;
RA Lemma-Gray P., Valusova E., Carroll C.A., Weintraub S.T., Musatov A.,
RA Robinson N.C.;
RT "Subunit analysis of bovine heart complex I by reversed-phase high-
RT performance liquid chromatography, electrospray ionization-tandem mass
RT spectrometry, and matrix-assisted laser desorption/ionization-time-of-
RT flight mass spectrometry.";
RL Anal. Biochem. 382:116-121(2008).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed not to be
CC involved in catalysis. Complex I functions in the transfer of electrons
CC from NADH to the respiratory chain. The immediate electron acceptor for
CC the enzyme is believed to be ubiquinone.
CC {ECO:0000250|UniProtKB:O95169}.
CC -!- SUBUNIT: Complex I is composed of 45 different subunits.
CC {ECO:0000269|PubMed:10852722, ECO:0000269|PubMed:1518044,
CC ECO:0000269|PubMed:18721790}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000305|PubMed:10852722, ECO:0000305|PubMed:18721790}; Single-pass
CC membrane protein {ECO:0000255}; Matrix side {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the complex I NDUFB8 subunit family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X63209; CAA44894.1; -; mRNA.
DR EMBL; AY911318; AAW82086.1; -; mRNA.
DR EMBL; BC103425; AAI03426.1; -; mRNA.
DR PIR; S28242; S28242.
DR RefSeq; NP_777092.1; NM_174667.3.
DR PDB; 7QSD; EM; 3.10 A; l=1-186.
DR PDBsum; 7QSD; -.
DR AlphaFoldDB; Q02372; -.
DR SMR; Q02372; -.
DR CORUM; Q02372; -.
DR DIP; DIP-38814N; -.
DR IntAct; Q02372; 2.
DR STRING; 9913.ENSBTAP00000000100; -.
DR TCDB; 3.D.1.6.1; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR PaxDb; Q02372; -.
DR Ensembl; ENSBTAT00000000100; ENSBTAP00000000100; ENSBTAG00000000091.
DR GeneID; 282517; -.
DR KEGG; bta:282517; -.
DR CTD; 4714; -.
DR VEuPathDB; HostDB:ENSBTAG00000000091; -.
DR eggNOG; KOG4040; Eukaryota.
DR GeneTree; ENSGT00390000000628; -.
DR HOGENOM; CLU_108654_1_0_1; -.
DR InParanoid; Q02372; -.
DR OMA; CFLGVMS; -.
DR OrthoDB; 1427659at2759; -.
DR TreeFam; TF317319; -.
DR Reactome; R-BTA-1268020; Mitochondrial protein import.
DR Reactome; R-BTA-611105; Respiratory electron transport.
DR Reactome; R-BTA-6799198; Complex I biogenesis.
DR Proteomes; UP000009136; Chromosome 26.
DR Bgee; ENSBTAG00000000091; Expressed in tongue muscle and 103 other tissues.
DR ExpressionAtlas; Q02372; baseline.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IDA:UniProtKB.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; IEA:InterPro.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR InterPro; IPR008699; NDUFB8.
DR InterPro; IPR016551; Ndufb8_metazoa.
DR PANTHER; PTHR12840; PTHR12840; 1.
DR Pfam; PF05821; NDUF_B8; 1.
DR PIRSF; PIRSF009288; NDUB8; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Electron transport; Membrane;
KW Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Respiratory chain; Transit peptide; Transmembrane; Transmembrane helix;
KW Transport.
FT TRANSIT 1..28
FT /note="Mitochondrion"
FT CHAIN 29..186
FT /note="NADH dehydrogenase [ubiquinone] 1 beta subcomplex
FT subunit 8, mitochondrial"
FT /id="PRO_0000020045"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 186 AA; 21652 MW; 523A3596D2F73E5C CRC64;
MAAARAGVLG VRWLQKAARN VVPLGARTAS HITKDMLPGP YPKTPEERAA AAKKYNMRVE
DYEPYPDDGT GYGDYPKLPD RSQQERDPWY DWDHPDLRLN WGEPMHWDLD MYIRNRVDTS
PTPVNWNLMC KHLFGFVAFM LFMFWVGETY PAYQPVGPKQ YPYNNLYLER GGDPNKEPEP
VVHYEI
