NDUB8_MOUSE
ID NDUB8_MOUSE Reviewed; 186 AA.
AC Q9D6J5; Q9D6M7;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial;
DE AltName: Full=Complex I-ASHI;
DE Short=CI-ASHI;
DE AltName: Full=NADH-ubiquinone oxidoreductase ASHI subunit;
DE Flags: Precursor;
GN Name=Ndufb8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Hippocampus, and Tongue;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PROTEIN SEQUENCE OF 35-43; 87-98 AND 160-186, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RA Lubec G., Kang S.U.;
RL Submitted (APR-2007) to UniProtKB.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed not to be
CC involved in catalysis. Complex I functions in the transfer of electrons
CC from NADH to the respiratory chain. The immediate electron acceptor for
CC the enzyme is believed to be ubiquinone.
CC {ECO:0000250|UniProtKB:O95169}.
CC -!- SUBUNIT: Complex I is composed of 45 different subunits.
CC {ECO:0000250|UniProtKB:O95169}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:O95169}; Single-pass membrane protein
CC {ECO:0000255}; Matrix side {ECO:0000250|UniProtKB:O95169}.
CC -!- SIMILARITY: Belongs to the complex I NDUFB8 subunit family.
CC {ECO:0000305}.
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DR EMBL; AK010177; BAB26749.1; -; mRNA.
DR EMBL; AK013516; BAB28893.1; -; mRNA.
DR EMBL; BC043019; AAH43019.1; -; mRNA.
DR CCDS; CCDS29851.1; -.
DR RefSeq; NP_080337.1; NM_026061.2.
DR PDB; 6G2J; EM; 3.30 A; l=1-186.
DR PDB; 6G72; EM; 3.90 A; l=1-186.
DR PDB; 6ZR2; EM; 3.10 A; l=1-186.
DR PDB; 6ZTQ; EM; 3.00 A; l=1-186.
DR PDB; 7AK5; EM; 3.17 A; l=1-186.
DR PDB; 7AK6; EM; 3.82 A; l=1-186.
DR PDB; 7B93; EM; 3.04 A; l=1-186.
DR PDB; 7PSA; EM; 3.40 A; l=1-186.
DR PDBsum; 6G2J; -.
DR PDBsum; 6G72; -.
DR PDBsum; 6ZR2; -.
DR PDBsum; 6ZTQ; -.
DR PDBsum; 7AK5; -.
DR PDBsum; 7AK6; -.
DR PDBsum; 7B93; -.
DR PDBsum; 7PSA; -.
DR AlphaFoldDB; Q9D6J5; -.
DR SMR; Q9D6J5; -.
DR BioGRID; 212057; 4.
DR ComplexPortal; CPX-266; Mitochondrial respiratory chain complex I.
DR CORUM; Q9D6J5; -.
DR IntAct; Q9D6J5; 4.
DR STRING; 10090.ENSMUSP00000026222; -.
DR iPTMnet; Q9D6J5; -.
DR PhosphoSitePlus; Q9D6J5; -.
DR SwissPalm; Q9D6J5; -.
DR EPD; Q9D6J5; -.
DR jPOST; Q9D6J5; -.
DR MaxQB; Q9D6J5; -.
DR PaxDb; Q9D6J5; -.
DR PeptideAtlas; Q9D6J5; -.
DR PRIDE; Q9D6J5; -.
DR ProteomicsDB; 286167; -.
DR DNASU; 67264; -.
DR Ensembl; ENSMUST00000026222; ENSMUSP00000026222; ENSMUSG00000025204.
DR GeneID; 67264; -.
DR KEGG; mmu:67264; -.
DR UCSC; uc008hpw.1; mouse.
DR CTD; 4714; -.
DR MGI; MGI:1914514; Ndufb8.
DR VEuPathDB; HostDB:ENSMUSG00000025204; -.
DR eggNOG; KOG4040; Eukaryota.
DR GeneTree; ENSGT00390000000628; -.
DR HOGENOM; CLU_108654_1_0_1; -.
DR InParanoid; Q9D6J5; -.
DR OMA; CFLGVMS; -.
DR OrthoDB; 1427659at2759; -.
DR PhylomeDB; Q9D6J5; -.
DR TreeFam; TF317319; -.
DR Reactome; R-MMU-1268020; Mitochondrial protein import.
DR Reactome; R-MMU-611105; Respiratory electron transport.
DR Reactome; R-MMU-6799198; Complex I biogenesis.
DR BioGRID-ORCS; 67264; 23 hits in 73 CRISPR screens.
DR ChiTaRS; Ndufb8; mouse.
DR PRO; PR:Q9D6J5; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q9D6J5; protein.
DR Bgee; ENSMUSG00000025204; Expressed in right kidney and 267 other tissues.
DR ExpressionAtlas; Q9D6J5; baseline and differential.
DR Genevisible; Q9D6J5; MM.
DR GO; GO:0005783; C:endoplasmic reticulum; ISO:MGI.
DR GO; GO:0005743; C:mitochondrial inner membrane; HDA:MGI.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IDA:MGI.
DR GO; GO:0005739; C:mitochondrion; IDA:MGI.
DR GO; GO:0009060; P:aerobic respiration; IC:ComplexPortal.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; IEA:InterPro.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR GO; GO:0042776; P:proton motive force-driven mitochondrial ATP synthesis; IC:ComplexPortal.
DR InterPro; IPR008699; NDUFB8.
DR InterPro; IPR016551; Ndufb8_metazoa.
DR PANTHER; PTHR12840; PTHR12840; 1.
DR Pfam; PF05821; NDUF_B8; 1.
DR PIRSF; PIRSF009288; NDUB8; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Electron transport; Membrane;
KW Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Respiratory chain; Transit peptide; Transmembrane; Transmembrane helix;
KW Transport.
FT TRANSIT 1..28
FT /note="Mitochondrion"
FT /evidence="ECO:0000250"
FT CHAIN 29..186
FT /note="NADH dehydrogenase [ubiquinone] 1 beta subcomplex
FT subunit 8, mitochondrial"
FT /id="PRO_0000020047"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 105
FT /note="I -> V (in Ref. 1; BAB26749)"
FT /evidence="ECO:0000305"
FT HELIX 34..36
FT /evidence="ECO:0007829|PDB:7B93"
FT HELIX 45..55
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 59..61
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 68..71
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 83..86
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 88..90
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 95..97
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 101..103
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 107..111
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 114..116
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 126..149
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 162..166
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 168..170
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 174..176
FT /evidence="ECO:0007829|PDB:7B93"
SQ SEQUENCE 186 AA; 21876 MW; DBD21FF09C9F90C1 CRC64;
MAAARAAALG VRWLQRTTRG VVPLEARRAF HMTKDMLPGS YPRTPEERAA AAKKYNMRVE
DYEPYPDDGM GYGDYPMLPN RSQHERDPWY QWDHSELRMN WGEPIHWDLD MYIRNRVDTS
PTPVSWDVMC KHLFGFVAFM VFMFWVGHVF PSYQPVGPKQ YPYNNLYLER GGDPTKEPEP
VVHYDI
