NDUB9_ARATH
ID NDUB9_ARATH Reviewed; 117 AA.
AC Q945M1; O65692; Q56Z32; Q8LDS9;
DT 27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9;
DE AltName: Full=B22 subunit of eukaryotic mitochondrial complex I;
DE AltName: Full=Complex I-B22;
DE Short=AtCIB22;
DE Short=CI-B22;
DE AltName: Full=NADH-ubiquinone oxidoreductase B22 subunit;
GN Name=CIB22; OrderedLocusNames=At4g34700; ORFNames=T4L20_280;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 58-117.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND SUBCELLULAR
RP LOCATION.
RX PubMed=21035093; DOI=10.1016/s1673-8527(09)60085-0;
RA Han L., Qin G., Kang D., Chen Z., Gu H., Qu L.J.;
RT "A nuclear-encoded mitochondrial gene AtCIB22 is essential for plant
RT development in Arabidopsis.";
RL J. Genet. Genomics 37:667-683(2010).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed to be not
CC involved in catalysis. Complex I functions in the transfer of electrons
CC from NADH to the respiratory chain. The immediate electron acceptor for
CC the enzyme is believed to be ubiquinone (By similarity). Is required
CC for correct plant growth and development. {ECO:0000250,
CC ECO:0000269|PubMed:21035093}.
CC -!- SUBUNIT: Complex I is composed of at least 49 different subunits.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:21035093}; Peripheral membrane protein
CC {ECO:0000269|PubMed:21035093}; Matrix side
CC {ECO:0000269|PubMed:21035093}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems, flowers, rosette leaves,
CC cauline leaves and siliques, with the highest expression in the stems.
CC {ECO:0000269|PubMed:21035093}.
CC -!- DISRUPTION PHENOTYPE: Shorter roots, smaller plants and delayed
CC flowering. {ECO:0000269|PubMed:21035093}.
CC -!- SIMILARITY: Belongs to the complex I LYR family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA18849.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB80187.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL023094; CAA18849.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161586; CAB80187.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE86412.1; -; Genomic_DNA.
DR EMBL; AF412069; AAL06522.1; -; mRNA.
DR EMBL; BT001011; AAN46765.1; -; mRNA.
DR EMBL; AY085825; AAM63041.1; -; mRNA.
DR EMBL; AK221137; BAD95113.1; -; mRNA.
DR PIR; T05290; T05290.
DR RefSeq; NP_567970.1; NM_119636.4.
DR PDB; 7A23; EM; 3.70 A; d=1-117.
DR PDB; 7AQW; EM; 3.17 A; n=1-117.
DR PDB; 7AR7; EM; 3.72 A; n=7-115.
DR PDB; 7AR8; EM; 3.53 A; n=1-117.
DR PDB; 7ARB; EM; 3.41 A; n=1-117.
DR PDBsum; 7A23; -.
DR PDBsum; 7AQW; -.
DR PDBsum; 7AR7; -.
DR PDBsum; 7AR8; -.
DR PDBsum; 7ARB; -.
DR AlphaFoldDB; Q945M1; -.
DR SMR; Q945M1; -.
DR BioGRID; 14904; 5.
DR IntAct; Q945M1; 3.
DR STRING; 3702.AT4G34700.1; -.
DR TCDB; 3.D.1.6.3; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR iPTMnet; Q945M1; -.
DR SwissPalm; Q945M1; -.
DR PaxDb; Q945M1; -.
DR PRIDE; Q945M1; -.
DR ProteomicsDB; 236821; -.
DR EnsemblPlants; AT4G34700.1; AT4G34700.1; AT4G34700.
DR GeneID; 829622; -.
DR Gramene; AT4G34700.1; AT4G34700.1; AT4G34700.
DR KEGG; ath:AT4G34700; -.
DR Araport; AT4G34700; -.
DR TAIR; locus:2139619; AT4G34700.
DR eggNOG; KOG3466; Eukaryota.
DR HOGENOM; CLU_108081_1_0_1; -.
DR InParanoid; Q945M1; -.
DR OMA; KWERNTP; -.
DR OrthoDB; 1553121at2759; -.
DR PhylomeDB; Q945M1; -.
DR PRO; PR:Q945M1; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q945M1; baseline and differential.
DR Genevisible; Q945M1; AT.
DR GO; GO:0031966; C:mitochondrial membrane; IDA:TAIR.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IDA:TAIR.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0045271; C:respiratory chain complex I; IDA:TAIR.
DR GO; GO:0005975; P:carbohydrate metabolic process; IMP:TAIR.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; IEA:InterPro.
DR GO; GO:0009853; P:photorespiration; TAS:TAIR.
DR CDD; cd20263; Complex1_LYR_NDUFB9_LYRM3; 1.
DR InterPro; IPR008011; Complex1_LYR_dom.
DR InterPro; IPR045292; Complex1_LYR_NDUFB9_LYRM3.
DR InterPro; IPR033034; NDUFB9.
DR PANTHER; PTHR12868; PTHR12868; 1.
DR Pfam; PF05347; Complex1_LYR; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Electron transport; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Respiratory chain;
KW Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CHAIN 2..117
FT /note="NADH dehydrogenase [ubiquinone] 1 beta subcomplex
FT subunit 9"
FT /id="PRO_0000410999"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CONFLICT 54
FT /note="V -> A (in Ref. 4; AAM63041)"
FT /evidence="ECO:0000305"
FT TURN 8..15
FT /evidence="ECO:0007829|PDB:7AQW"
FT HELIX 16..34
FT /evidence="ECO:0007829|PDB:7AQW"
FT HELIX 38..55
FT /evidence="ECO:0007829|PDB:7AQW"
FT HELIX 61..78
FT /evidence="ECO:0007829|PDB:7AQW"
FT STRAND 92..94
FT /evidence="ECO:0007829|PDB:7AQW"
SQ SEQUENCE 117 AA; 13616 MW; 37DE865F28C54DC3 CRC64;
MSGVSTAAYF ARRAAQKERV RILYRRALKD TLNWAVHRHI FYRDASDLRE KFNVNQDVED
VDRIDKLIAH GEAEYNKWRH PDPYIVPWAP GGSKFCRNPT PPAGIEIVYN YGLEDNP
