NDUB9_MOUSE
ID NDUB9_MOUSE Reviewed; 179 AA.
AC Q9CQJ8;
DT 26-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9;
DE AltName: Full=Complex I-B22;
DE Short=CI-B22;
DE AltName: Full=NADH-ubiquinone oxidoreductase B22 subunit;
GN Name=Ndufb9;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Kidney;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PROTEIN SEQUENCE OF 26-34; 39-45; 48-55; 99-121; 139-158 AND 160-175, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RA Lubec G., Kang S.U.;
RL Submitted (APR-2007) to UniProtKB.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed to be not
CC involved in catalysis. Complex I functions in the transfer of electrons
CC from NADH to the respiratory chain. The immediate electron acceptor for
CC the enzyme is believed to be ubiquinone.
CC {ECO:0000250|UniProtKB:Q9Y6M9}.
CC -!- SUBUNIT: Mammalian complex I is composed of 45 different subunits.
CC {ECO:0000250|UniProtKB:Q9Y6M9}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:Q9Y6M9}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:Q9Y6M9}; Matrix side
CC {ECO:0000250|UniProtKB:Q9Y6M9}.
CC -!- SIMILARITY: Belongs to the complex I LYR family. {ECO:0000305}.
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DR EMBL; AK002254; BAB21968.1; -; mRNA.
DR EMBL; AK003540; BAB22846.1; -; mRNA.
DR EMBL; AK004522; BAB23345.1; -; mRNA.
DR EMBL; BC031539; AAH31539.1; -; mRNA.
DR CCDS; CCDS27495.1; -.
DR RefSeq; NP_075661.1; NM_023172.3.
DR PDB; 6G2J; EM; 3.30 A; n=1-179.
DR PDB; 6G72; EM; 3.90 A; n=1-179.
DR PDB; 6ZR2; EM; 3.10 A; n=1-179.
DR PDB; 6ZTQ; EM; 3.00 A; n=1-179.
DR PDB; 7AK5; EM; 3.17 A; n=1-179.
DR PDB; 7AK6; EM; 3.82 A; n=1-179.
DR PDB; 7B93; EM; 3.04 A; n=1-179.
DR PDB; 7PSA; EM; 3.40 A; n=1-179.
DR PDBsum; 6G2J; -.
DR PDBsum; 6G72; -.
DR PDBsum; 6ZR2; -.
DR PDBsum; 6ZTQ; -.
DR PDBsum; 7AK5; -.
DR PDBsum; 7AK6; -.
DR PDBsum; 7B93; -.
DR PDBsum; 7PSA; -.
DR AlphaFoldDB; Q9CQJ8; -.
DR SMR; Q9CQJ8; -.
DR BioGRID; 211305; 8.
DR ComplexPortal; CPX-266; Mitochondrial respiratory chain complex I.
DR CORUM; Q9CQJ8; -.
DR IntAct; Q9CQJ8; 3.
DR STRING; 10090.ENSMUSP00000022980; -.
DR iPTMnet; Q9CQJ8; -.
DR PhosphoSitePlus; Q9CQJ8; -.
DR SwissPalm; Q9CQJ8; -.
DR EPD; Q9CQJ8; -.
DR jPOST; Q9CQJ8; -.
DR MaxQB; Q9CQJ8; -.
DR PaxDb; Q9CQJ8; -.
DR PeptideAtlas; Q9CQJ8; -.
DR PRIDE; Q9CQJ8; -.
DR ProteomicsDB; 252943; -.
DR Antibodypedia; 27088; 350 antibodies from 34 providers.
DR DNASU; 66218; -.
DR Ensembl; ENSMUST00000022980; ENSMUSP00000022980; ENSMUSG00000022354.
DR GeneID; 66218; -.
DR KEGG; mmu:66218; -.
DR UCSC; uc007vtw.1; mouse.
DR CTD; 4715; -.
DR MGI; MGI:1913468; Ndufb9.
DR VEuPathDB; HostDB:ENSMUSG00000022354; -.
DR eggNOG; KOG3466; Eukaryota.
DR GeneTree; ENSGT00390000005809; -.
DR HOGENOM; CLU_108081_0_0_1; -.
DR InParanoid; Q9CQJ8; -.
DR OMA; QMRARFD; -.
DR OrthoDB; 1553121at2759; -.
DR PhylomeDB; Q9CQJ8; -.
DR TreeFam; TF315148; -.
DR Reactome; R-MMU-611105; Respiratory electron transport.
DR Reactome; R-MMU-6799198; Complex I biogenesis.
DR BioGRID-ORCS; 66218; 24 hits in 72 CRISPR screens.
DR ChiTaRS; Ndufb9; mouse.
DR PRO; PR:Q9CQJ8; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q9CQJ8; protein.
DR Bgee; ENSMUSG00000022354; Expressed in facial nucleus and 252 other tissues.
DR Genevisible; Q9CQJ8; MM.
DR GO; GO:0005743; C:mitochondrial inner membrane; HDA:MGI.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0009060; P:aerobic respiration; IC:ComplexPortal.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; IEA:InterPro.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR GO; GO:0042776; P:proton motive force-driven mitochondrial ATP synthesis; IC:ComplexPortal.
DR CDD; cd20263; Complex1_LYR_NDUFB9_LYRM3; 1.
DR InterPro; IPR008011; Complex1_LYR_dom.
DR InterPro; IPR045292; Complex1_LYR_NDUFB9_LYRM3.
DR InterPro; IPR033034; NDUFB9.
DR PANTHER; PTHR12868; PTHR12868; 1.
DR Pfam; PF05347; Complex1_LYR; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Direct protein sequencing; Electron transport;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Phosphoprotein;
KW Reference proteome; Respiratory chain; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6M9"
FT CHAIN 2..179
FT /note="NADH dehydrogenase [ubiquinone] 1 beta subcomplex
FT subunit 9"
FT /id="PRO_0000174307"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6M9"
FT MOD_RES 85
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6M9"
FT HELIX 12..30
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 34..49
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 51..53
FT /evidence="ECO:0007829|PDB:7B93"
FT TURN 57..59
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 60..72
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 85..88
FT /evidence="ECO:0007829|PDB:6G2J"
FT TURN 89..92
FT /evidence="ECO:0007829|PDB:7AK5"
FT HELIX 93..97
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 102..106
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 109..111
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 112..114
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 116..141
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 142..144
FT /evidence="ECO:0007829|PDB:6G2J"
FT STRAND 159..161
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 168..172
FT /evidence="ECO:0007829|PDB:6ZTQ"
SQ SEQUENCE 179 AA; 21984 MW; 826692A25D5B9B78 CRC64;
MAFCAPPAYL THQQKVLRLY KRALRHLESW CIHRDKYRYF ACLMRARFEE HKNEKDMMRA
TQLLREAEEE FWQNQHPQPY IFPDSPGGTS FERYECYKVP EWCLDYWHPS EKAMYPDYFS
KREQWKKLRM ESWDREVKQL QEETSPDGIM TEALPPARRE GDLPPLWWHI VTRPRERPT
