ID   13S2_FAGES              Reviewed;         504 AA.
AC   O23880;
DT   11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=13S globulin seed storage protein 2;
DE   AltName: Full=Legumin-like protein 2;
DE   Contains:
DE     RecName: Full=13S globulin seed storage protein 2 acidic chain;
DE   Contains:
DE     RecName: Full=13S globulin seed storage protein 2 basic chain;
DE   Flags: Precursor;
GN   Name=FA18;
OS   Fagopyrum esculentum (Common buckwheat) (Polygonum fagopyrum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Polygonaceae; Polygonoideae; Fagopyreae; Fagopyrum.
OX   NCBI_TaxID=3617;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Kitayuki; TISSUE=Immature seed;
RX   PubMed=11308332; DOI=10.1021/jf0011485;
RA   Fujino K., Funatsuki H., Inada M., Shimono Y., Kikuta Y.;
RT   "Expression, cloning, and immunological analysis of buckwheat (Fagopyrum
RT   esculentum Moench) seed storage proteins.";
RL   J. Agric. Food Chem. 49:1825-1829(2001).
CC   -!- FUNCTION: Seed storage protein.
CC   -!- SUBUNIT: Hexamer; each subunit is composed of an acidic and a basic
CC       chain derived from a single precursor and linked by a disulfide bond.
CC       {ECO:0000250}.
CC   -!- MISCELLANEOUS: The sequence of the probable beta chain is highly
CC       homologous to the N-terminal sequence of BW24KD, a major buckwheat
CC       allergen.
CC   -!- SIMILARITY: Belongs to the 11S seed storage protein (globulins) family.
CC       {ECO:0000305}.
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DR   EMBL; D87982; BAA21760.1; -; mRNA.
DR   PIR; T10698; T10698.
DR   AlphaFoldDB; O23880; -.
DR   SMR; O23880; -.
DR   Allergome; 698; Fag e 1.
DR   PRIDE; O23880; -.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.120.10; -; 2.
DR   InterPro; IPR006044; 11S_seedstore_pln.
DR   InterPro; IPR006045; Cupin_1.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   Pfam; PF00190; Cupin_1; 2.
DR   PRINTS; PR00439; 11SGLOBULIN.
DR   SMART; SM00835; Cupin_1; 2.
DR   SUPFAM; SSF51182; SSF51182; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Seed storage protein; Signal; Storage protein.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..313
FT                   /note="13S globulin seed storage protein 2 acidic chain"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000032005"
FT   CHAIN           314..504
FT                   /note="13S globulin seed storage protein 2 basic chain"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000032006"
FT   DOMAIN          51..265
FT                   /note="Cupin type-1 1"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          326..475
FT                   /note="Cupin type-1 2"
FT                   /evidence="ECO:0000255"
FT   REGION          128..158
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          214..237
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          289..314
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        135..158
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        219..237
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        289..306
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   DISULFID        46..79
FT                   /evidence="ECO:0000250"
FT   DISULFID        122..320
FT                   /note="Interchain (between acidic and basic chains)"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   504 AA;  57043 MW;  CDCA322394A28194 CRC64;
     MSTKLILSFS LCLMVLSCSA QLWPWQKGQG SRPHHGRQQH QFQHQCDIQR LTASEPSRRV
     RSEAGVTEIW DHDTPEFRCT GFVAVRVVIQ PGGLLLPSYS NAPYITFVEQ GRGVQGVVIP
     GCPETFQSDS EFEYPQSQRG RHSRQSESEE ESSRGDQHQK IFRIREGDVI PSPAGVVQWT
     HNDGNDDLIS VTLLDANSYH KQLDENVRSF FLAGQSQRET REEGSDRQSR ESDDDEALLG
     ANILSGFQDE ILHELFRDVD RETISKLRGE NDQRGFIVQA QDLKLRVPQD FEEEYERERG
     DRRRGQGGSG RSNGVEQGFC NLKFRRNFNT PTNTYVFNPR AGRINTVNSN SLPILEFLQL
     SAQHVVLYKN AIIGPRWNLN AHSALYVTRG EGRVQVVGDE GKSVFDDKVQ RGQILVVPQG
     FAVVLKAGRE GLEWVELKNS GNAITSPIGG RTSVLRAIPV EVLANSYDIS TKEAYKLKNG
     RQEVEVFRPF QSRDEKERER FSIV