NDUC2_BOVIN
ID NDUC2_BOVIN Reviewed; 120 AA.
AC Q02827; Q3SZR4;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 subunit C2 {ECO:0000250|UniProtKB:O95298};
DE AltName: Full=Complex I-B14.5b;
DE Short=CI-B14.5b;
DE AltName: Full=NADH-ubiquinone oxidoreductase subunit B14.5b;
GN Name=NDUFC2 {ECO:0000250|UniProtKB:O95298};
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, AND ACETYLATION AT
RP MET-1.
RC TISSUE=Heart;
RX PubMed=1426273; DOI=10.1016/0014-5793(92)81189-s;
RA Arizmendi J.M., Skehel J.M., Runswick M.J., Fearnley I.M., Walker J.E.;
RT "Complementary DNA sequences of two 14.5 kDa subunits of NADH:ubiquinone
RT oxidoreductase from bovine heart mitochondria. Completion of the primary
RT structure of the complex?";
RL FEBS Lett. 313:80-84(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Testis;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP SUBUNIT, IDENTIFICATION IN COMPLEX I, AND SUBCELLULAR LOCATION.
RX PubMed=18721790; DOI=10.1016/j.ab.2008.07.029;
RA Lemma-Gray P., Valusova E., Carroll C.A., Weintraub S.T., Musatov A.,
RA Robinson N.C.;
RT "Subunit analysis of bovine heart complex I by reversed-phase high-
RT performance liquid chromatography, electrospray ionization-tandem mass
RT spectrometry, and matrix-assisted laser desorption/ionization-time-of-
RT flight mass spectrometry.";
RL Anal. Biochem. 382:116-121(2008).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed not to be
CC involved in catalysis but required for the complex assembly. Complex I
CC functions in the transfer of electrons from NADH to the respiratory
CC chain. The immediate electron acceptor for the enzyme is believed to be
CC ubiquinone. {ECO:0000250|UniProtKB:O95298}.
CC -!- SUBUNIT: Complex I is composed of 45 different subunits. Interacts with
CC TMEM242 (By similarity). {ECO:0000250|UniProtKB:O95298,
CC ECO:0000269|PubMed:18721790}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000305|PubMed:18721790}; Single-pass membrane protein
CC {ECO:0000255}; Matrix side {ECO:0000305}.
CC -!- PTM: There is a minor unacetylated form of subunit B14.5b.
CC {ECO:0000269|PubMed:1426273}.
CC -!- SIMILARITY: Belongs to the complex I NDUFC2 subunit family.
CC {ECO:0000305}.
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DR EMBL; X68647; CAA48607.1; -; mRNA.
DR EMBL; BC102739; AAI02740.1; -; mRNA.
DR PIR; S27227; S27227.
DR RefSeq; NP_788815.1; NM_176642.3.
DR PDB; 5LC5; EM; 4.35 A; d=29-97.
DR PDB; 5LDW; EM; 4.27 A; d=29-96.
DR PDB; 5LDX; EM; 5.60 A; d=29-97.
DR PDB; 5O31; EM; 4.13 A; d=1-97.
DR PDB; 7QSD; EM; 3.10 A; d=1-120.
DR PDBsum; 5LC5; -.
DR PDBsum; 5LDW; -.
DR PDBsum; 5LDX; -.
DR PDBsum; 5O31; -.
DR PDBsum; 7QSD; -.
DR AlphaFoldDB; Q02827; -.
DR SMR; Q02827; -.
DR CORUM; Q02827; -.
DR DIP; DIP-38794N; -.
DR IntAct; Q02827; 1.
DR STRING; 9913.ENSBTAP00000024209; -.
DR TCDB; 3.D.1.6.1; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR iPTMnet; Q02827; -.
DR PaxDb; Q02827; -.
DR PRIDE; Q02827; -.
DR Ensembl; ENSBTAT00000024209; ENSBTAP00000024209; ENSBTAG00000018188.
DR GeneID; 338046; -.
DR KEGG; bta:338046; -.
DR CTD; 4718; -.
DR VEuPathDB; HostDB:ENSBTAG00000018188; -.
DR eggNOG; KOG4516; Eukaryota.
DR GeneTree; ENSGT00390000010352; -.
DR HOGENOM; CLU_156652_0_0_1; -.
DR InParanoid; Q02827; -.
DR OMA; WFIGYHL; -.
DR OrthoDB; 1606491at2759; -.
DR TreeFam; TF314723; -.
DR Reactome; R-BTA-611105; Respiratory electron transport.
DR Reactome; R-BTA-6798695; Neutrophil degranulation.
DR Reactome; R-BTA-6799198; Complex I biogenesis.
DR Proteomes; UP000009136; Chromosome 29.
DR Bgee; ENSBTAG00000018188; Expressed in tongue muscle and 104 other tissues.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IDA:UniProtKB.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; IEA:InterPro.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR InterPro; IPR009423; NDUC2.
DR PANTHER; PTHR13099; PTHR13099; 1.
DR Pfam; PF06374; NDUF_C2; 1.
DR PIRSF; PIRSF017834; NADH-UbQ_OxRdtase_b14.5b; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Direct protein sequencing; Electron transport;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Respiratory chain; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..120
FT /note="NADH dehydrogenase [ubiquinone] 1 subunit C2"
FT /id="PRO_0000118836"
FT TRANSMEM 57..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000269|PubMed:1426273"
SQ SEQUENCE 120 AA; 14096 MW; AF35707C2C3F2B89 CRC64;
MMTGRQGRAT FQFLPDEARS LPPPKLTDPR LAFVGFLGYC SGLIDNAIRR RPVLLAGLHR
QLLYITSFVF VGYYLLKRQD YMYAVRDHDM FSYIKSHPED FPEKDKKTYG EVFEEFHPVR
