ID   NDUF3_RAT               Reviewed;         185 AA.
AC   O08776; O08777; Q6AXV1; Q78E24;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3;
DE   AltName: Full=Nuclear protein E3-3;
GN   Name=Ndufaf3;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), SUBCELLULAR LOCATION, AND
RP   TISSUE SPECIFICITY.
RC   TISSUE=Testis;
RX   PubMed=9349717; DOI=10.1016/s0167-4781(97)00090-0;
RA   Hartmann A.M., Stamm S.;
RT   "Molecular cloning of a novel alternatively spliced nuclear protein.";
RL   Biochim. Biophys. Acta 1353:224-230(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Essential factor for the assembly of mitochondrial
CC       NADH:ubiquinone oxidoreductase complex (complex I). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with NDUFAF4, NDUFS2 and NDUFS3. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250}.
CC       Nucleus {ECO:0000269|PubMed:9349717}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=4;
CC       Name=1;
CC         IsoId=O08776-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=O08776-2; Sequence=VSP_023993, VSP_023996;
CC       Name=3;
CC         IsoId=O08776-3; Sequence=VSP_023994, VSP_023995;
CC       Name=4;
CC         IsoId=O08776-4; Sequence=VSP_023992;
CC   -!- TISSUE SPECIFICITY: Expressed in testis. {ECO:0000269|PubMed:9349717}.
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DR   EMBL; U95160; AAB54063.1; -; mRNA.
DR   EMBL; U95161; AAB54064.1; -; mRNA.
DR   EMBL; U95162; AAB54065.1; -; mRNA.
DR   EMBL; BC079306; AAH79306.1; -; mRNA.
DR   RefSeq; NP_001029143.1; NM_001033971.2. [O08776-4]
DR   RefSeq; NP_064465.1; NM_020080.3. [O08776-1]
DR   AlphaFoldDB; O08776; -.
DR   SMR; O08776; -.
DR   IntAct; O08776; 2.
DR   STRING; 10116.ENSRNOP00000027262; -.
DR   PaxDb; O08776; -.
DR   PRIDE; O08776; -.
DR   Ensembl; ENSRNOT00000027262; ENSRNOP00000027262; ENSRNOG00000020068. [O08776-4]
DR   Ensembl; ENSRNOT00000110739; ENSRNOP00000091967; ENSRNOG00000020068. [O08776-1]
DR   GeneID; 56769; -.
DR   KEGG; rno:56769; -.
DR   CTD; 25915; -.
DR   RGD; 708545; Ndufaf3.
DR   eggNOG; KOG3363; Eukaryota.
DR   GeneTree; ENSGT00390000018312; -.
DR   HOGENOM; CLU_074390_3_1_1; -.
DR   InParanoid; O08776; -.
DR   OMA; FSKAYDH; -.
DR   OrthoDB; 1569969at2759; -.
DR   PhylomeDB; O08776; -.
DR   TreeFam; TF321072; -.
DR   Reactome; R-RNO-6799198; Complex I biogenesis.
DR   PRO; PR:O08776; -.
DR   Proteomes; UP000002494; Chromosome 8.
DR   Bgee; ENSRNOG00000020068; Expressed in testis and 20 other tissues.
DR   Genevisible; O08776; RN.
DR   GO; GO:0005743; C:mitochondrial inner membrane; ISO:RGD.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISO:RGD.
DR   CDD; cd05125; Mth938_2P1-like; 1.
DR   Gene3D; 3.40.1230.10; -; 1.
DR   InterPro; IPR036748; MTH938-like_sf.
DR   InterPro; IPR034095; NDUF3.
DR   InterPro; IPR007523; NDUFAF3/AAMDC.
DR   PANTHER; PTHR21192; PTHR21192; 1.
DR   Pfam; PF04430; DUF498; 1.
DR   SUPFAM; SSF64076; SSF64076; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Membrane; Mitochondrion;
KW   Mitochondrion inner membrane; Nucleus; Reference proteome.
FT   CHAIN           1..185
FT                   /note="NADH dehydrogenase [ubiquinone] 1 alpha subcomplex
FT                   assembly factor 3"
FT                   /id="PRO_0000281158"
FT   VAR_SEQ         21..26
FT                   /note="Missing (in isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_023992"
FT   VAR_SEQ         93..109
FT                   /note="GSHQDITEESFSIFWML -> SPPLHCNLYVVPKATPS (in isoform
FT                   3)"
FT                   /evidence="ECO:0000303|PubMed:9349717"
FT                   /id="VSP_023994"
FT   VAR_SEQ         93..100
FT                   /note="GSHQDITE -> SPPLHCNL (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:9349717"
FT                   /id="VSP_023993"
FT   VAR_SEQ         101..185
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:9349717"
FT                   /id="VSP_023996"
FT   VAR_SEQ         110..185
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:9349717"
FT                   /id="VSP_023995"
SQ   SEQUENCE   185 AA;  20696 MW;  B47AFEF6D0D43C14 CRC64;
     MATALGFRCL YRTRPATLGR YVDRLWRSPS RGHRLSPADD ELYQRTRISL LQNEFPQAVY
     IDSYNSRGFT INGNRVFGPC ALLPQTVVQW NVGSHQDITE ESFSIFWMLE PRIEIVVVGT
     GNKTERLHSQ VLQAMRQRGI AVEIQDTPNA CATFNFLCHE GRVTGAALIP PPGETALASS
     GQTTE