NDUF4_MOUSE
ID NDUF4_MOUSE Reviewed; 173 AA.
AC Q9D1H6; Q3TV72;
DT 16-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4;
DE AltName: Full=Hormone-regulated proliferation-associated protein of 20 kDa homolog;
GN Name=Ndufaf4; Synonyms=Hrpap20;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Urinary bladder;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May be involved in cell proliferation and survival of
CC hormone-dependent tumor cells. Involved in the assembly of
CC mitochondrial NADH:ubiquinone oxidoreductase complex (complex I) (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Binds calmodulin. Interacts with NDUFAF3. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}. Membrane
CC {ECO:0000250|UniProtKB:Q9P032}; Lipid-anchor
CC {ECO:0000250|UniProtKB:Q9P032}.
CC -!- PTM: Phosphorylated on serine. Prolactin stimulate serine
CC phosphorylation (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the NDUFAF4 family. {ECO:0000305}.
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DR EMBL; AK003553; BAB22852.1; -; mRNA.
DR EMBL; AK034728; BAC28809.1; -; mRNA.
DR EMBL; AK160341; BAE35747.1; -; mRNA.
DR EMBL; BC028764; AAH28764.1; -; mRNA.
DR CCDS; CCDS18007.1; -.
DR RefSeq; NP_081018.1; NM_026742.4.
DR AlphaFoldDB; Q9D1H6; -.
DR SMR; Q9D1H6; -.
DR BioGRID; 212884; 1.
DR STRING; 10090.ENSMUSP00000029925; -.
DR iPTMnet; Q9D1H6; -.
DR PhosphoSitePlus; Q9D1H6; -.
DR EPD; Q9D1H6; -.
DR jPOST; Q9D1H6; -.
DR MaxQB; Q9D1H6; -.
DR PaxDb; Q9D1H6; -.
DR PeptideAtlas; Q9D1H6; -.
DR PRIDE; Q9D1H6; -.
DR ProteomicsDB; 252870; -.
DR Antibodypedia; 48392; 126 antibodies from 25 providers.
DR DNASU; 68493; -.
DR Ensembl; ENSMUST00000029925; ENSMUSP00000029925; ENSMUSG00000028261.
DR GeneID; 68493; -.
DR KEGG; mmu:68493; -.
DR UCSC; uc008sed.2; mouse.
DR CTD; 29078; -.
DR MGI; MGI:1915743; Ndufaf4.
DR VEuPathDB; HostDB:ENSMUSG00000028261; -.
DR eggNOG; KOG4481; Eukaryota.
DR GeneTree; ENSGT00390000001627; -.
DR HOGENOM; CLU_054693_2_0_1; -.
DR InParanoid; Q9D1H6; -.
DR OMA; IPDQKYK; -.
DR OrthoDB; 1395543at2759; -.
DR PhylomeDB; Q9D1H6; -.
DR TreeFam; TF323532; -.
DR Reactome; R-MMU-6799198; Complex I biogenesis.
DR BioGRID-ORCS; 68493; 16 hits in 71 CRISPR screens.
DR ChiTaRS; Ndufaf4; mouse.
DR PRO; PR:Q9D1H6; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q9D1H6; protein.
DR Bgee; ENSMUSG00000028261; Expressed in atrioventricular valve and 259 other tissues.
DR ExpressionAtlas; Q9D1H6; baseline and differential.
DR Genevisible; Q9D1H6; MM.
DR GO; GO:0031966; C:mitochondrial membrane; ISO:MGI.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; ISO:MGI.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISO:MGI.
DR GO; GO:0010257; P:NADH dehydrogenase complex assembly; IMP:MGI.
DR GO; GO:0043066; P:negative regulation of apoptotic process; ISO:MGI.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:MGI.
DR InterPro; IPR009622; NDUFAF4.
DR PANTHER; PTHR13338; PTHR13338; 1.
DR Pfam; PF06784; UPF0240; 1.
PE 1: Evidence at protein level;
KW Calmodulin-binding; Lipoprotein; Membrane; Mitochondrion; Myristate;
KW Phosphoprotein; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q9P032"
FT CHAIN 2..173
FT /note="NADH dehydrogenase [ubiquinone] 1 alpha subcomplex
FT assembly factor 4"
FT /id="PRO_0000220989"
FT REGION 16..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 35
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9P032"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000250|UniProtKB:Q9P032"
SQ SEQUENCE 173 AA; 20082 MW; 80EEED8C3E3CBF6B CRC64;
MGARVTRALR NFNVEKRAER EISKRKPSMA PKHPSTRDLL QEHRSQYPEI EEVVSKKDNK
LLSLLRDVYV DSKDPVPALP VKVEPRQEPK EFRLPIGNHF DKNITDIPKG KITVVEALTL
LNNHKLSPET WTAEKIAQEY YLELKDVNSL LKYFVTFEVK ILPPEDRKAI QSK
