ID   NDUF4_RAT               Reviewed;         174 AA.
AC   Q9NQR8;
DT   01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4;
DE   AltName: Full=Hormone-regulated proliferation-associated protein of 20 kDa homolog;
DE   AltName: Full=Protein HRPAP20;
GN   Name=Ndufaf4; Synonyms=Hrpap20;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, AND PHOSPHORYLATION.
RX   PubMed=14871833; DOI=10.1158/0008-5472.can-03-0023;
RA   Karp C.M., Pan H., Zhang M., Buckley D.J., Schuler L.A., Buckley A.R.;
RT   "Identification of HRPAP20: a novel phosphoprotein that enhances growth and
RT   survival in hormone-responsive tumor cells.";
RL   Cancer Res. 64:1016-1025(2004).
CC   -!- FUNCTION: May be involved in cell proliferation and survival of
CC       hormone-dependent tumor cells. Involved in the assembly of
CC       mitochondrial NADH:ubiquinone oxidoreductase complex (complex I) (By
CC       similarity). {ECO:0000250, ECO:0000269|PubMed:14871833}.
CC   -!- SUBUNIT: Binds calmodulin. Interacts with NDUFAF3. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}. Membrane
CC       {ECO:0000250|UniProtKB:Q9P032}; Lipid-anchor
CC       {ECO:0000250|UniProtKB:Q9P032}.
CC   -!- INDUCTION: Expression is low in quiescent cells and is induced in
CC       exponentially proliferating cultures. Expression is also induced when
CC       prolactin is added to stationary cells. Induced by dietary
CC       differentiating agents such as butyrate, vitamin D3, and retinoic acid.
CC       {ECO:0000269|PubMed:14871833}.
CC   -!- PTM: Phosphorylated on serine. Prolactin stimulate serine
CC       phosphorylation. {ECO:0000269|PubMed:14871833}.
CC   -!- SIMILARITY: Belongs to the NDUFAF4 family. {ECO:0000305}.
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DR   EMBL; AF283900; AAF90196.1; -; mRNA.
DR   RefSeq; NP_942078.1; NM_198783.1.
DR   AlphaFoldDB; Q9NQR8; -.
DR   SMR; Q9NQR8; -.
DR   IntAct; Q9NQR8; 1.
DR   STRING; 10116.ENSRNOP00000010072; -.
DR   jPOST; Q9NQR8; -.
DR   PaxDb; Q9NQR8; -.
DR   PRIDE; Q9NQR8; -.
DR   Ensembl; ENSRNOT00000101158; ENSRNOP00000076552; ENSRNOG00000007506.
DR   GeneID; 362495; -.
DR   KEGG; rno:362495; -.
DR   UCSC; RGD:735162; rat.
DR   CTD; 29078; -.
DR   RGD; 735162; Ndufaf4.
DR   eggNOG; KOG4481; Eukaryota.
DR   GeneTree; ENSGT00390000001627; -.
DR   HOGENOM; CLU_054693_2_0_1; -.
DR   InParanoid; Q9NQR8; -.
DR   OMA; IPDQKYK; -.
DR   OrthoDB; 1395543at2759; -.
DR   PhylomeDB; Q9NQR8; -.
DR   TreeFam; TF323532; -.
DR   Reactome; R-RNO-6799198; Complex I biogenesis.
DR   PRO; PR:Q9NQR8; -.
DR   Proteomes; UP000002494; Chromosome 5.
DR   Bgee; ENSRNOG00000007506; Expressed in heart and 20 other tissues.
DR   Genevisible; Q9NQR8; RN.
DR   GO; GO:0031966; C:mitochondrial membrane; ISO:RGD.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; ISO:RGD.
DR   GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISO:RGD.
DR   GO; GO:0010257; P:NADH dehydrogenase complex assembly; ISO:RGD.
DR   GO; GO:0043066; P:negative regulation of apoptotic process; IMP:RGD.
DR   GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:RGD.
DR   InterPro; IPR009622; NDUFAF4.
DR   PANTHER; PTHR13338; PTHR13338; 1.
DR   Pfam; PF06784; UPF0240; 1.
PE   1: Evidence at protein level;
KW   Calmodulin-binding; Lipoprotein; Membrane; Mitochondrion; Myristate;
KW   Phosphoprotein; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:Q9P032"
FT   CHAIN           2..174
FT                   /note="NADH dehydrogenase [ubiquinone] 1 alpha subcomplex
FT                   assembly factor 4"
FT                   /id="PRO_0000342666"
FT   REGION          15..38
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         35
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9P032"
FT   LIPID           2
FT                   /note="N-myristoyl glycine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9P032"
SQ   SEQUENCE   174 AA;  20158 MW;  AC93F13A4EBABB5F CRC64;
     MGARMTRAFR NFNVEKRAEQ EISKRKPSMA PKHPSTRSLL QEHLSQYPEI EEEVSRKDNK
     LLSLLRDVYV NSKDPVPSLP VKAIEPQQQP KEFRLPIGDQ FDKNIKDIPK GKITVVEALT
     LLNNHKLSPE TWTAEKIAQE YHLELEDVNS LLKYFVTFEV KIVPPEDRKA IQSK