NDUF5_DICDI
ID NDUF5_DICDI Reviewed; 436 AA.
AC Q54JW0;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=Arginine-hydroxylase NDUFAF5, mitochondrial;
DE EC=1.-.-.- {ECO:0000250|UniProtKB:Q5TEU4};
DE AltName: Full=NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 {ECO:0000250|UniProtKB:Q5TEU4};
DE AltName: Full=Putative methyltransferase NDUFAF5 {ECO:0000305};
DE EC=2.1.1.- {ECO:0000305};
DE Flags: Precursor;
GN Name=ndufaf5 {ECO:0000303|PubMed:23536703}; ORFNames=DDB_G0287769;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP GLY-86; LEU-165 AND LEU-235.
RX PubMed=23536703; DOI=10.1091/mbc.e12-11-0796;
RA Carilla-Latorre S., Annesley S.J., Munoz-Braceras S., Fisher P.R.,
RA Escalante R.;
RT "Ndufaf5 deficiency in the Dictyostelium model: new roles in autophagy and
RT development.";
RL Mol. Biol. Cell 24:1519-1528(2013).
CC -!- FUNCTION: Involved in the assembly of mitochondrial NADH:ubiquinone
CC oxidoreductase complex (complex I, MT-ND1) at early stages
CC (PubMed:23536703). Probably acts as an arginine hydroxylase (By
CC similarity). May also have methyltransferase activity.
CC {ECO:0000250|UniProtKB:Q5TEU4, ECO:0000269|PubMed:23536703}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:23536703}.
CC -!- DISRUPTION PHENOTYPE: Defects in development due to mitochondrial
CC NADH:ubiquinone oxidoreductase complex (complex I, MT-ND1) deficiency
CC (PubMed:23536703). Increased autophagy (PubMed:23536703).
CC {ECO:0000269|PubMed:23536703}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC {ECO:0000305}.
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DR EMBL; AAFI02000104; EAL63531.1; -; Genomic_DNA.
DR RefSeq; XP_637042.1; XM_631950.1.
DR AlphaFoldDB; Q54JW0; -.
DR SMR; Q54JW0; -.
DR STRING; 44689.DDB0187624; -.
DR PaxDb; Q54JW0; -.
DR EnsemblProtists; EAL63531; EAL63531; DDB_G0287769.
DR GeneID; 8626296; -.
DR KEGG; ddi:DDB_G0287769; -.
DR dictyBase; DDB_G0287769; ndufaf5.
DR eggNOG; KOG2940; Eukaryota.
DR HOGENOM; CLU_046586_0_0_1; -.
DR InParanoid; Q54JW0; -.
DR OMA; QNMGENN; -.
DR PhylomeDB; Q54JW0; -.
DR PRO; PR:Q54JW0; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0005739; C:mitochondrion; IDA:dictyBase.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0031154; P:culmination involved in sorocarp development; IMP:dictyBase.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; IMP:dictyBase.
DR GO; GO:0010507; P:negative regulation of autophagy; IMP:dictyBase.
DR GO; GO:0042331; P:phototaxis; IMP:dictyBase.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IMP:dictyBase.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 1: Evidence at protein level;
KW Methyltransferase; Mitochondrion; Oxidoreductase; Reference proteome;
KW Transferase; Transit peptide.
FT TRANSIT 1..25
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 26..436
FT /note="Arginine-hydroxylase NDUFAF5, mitochondrial"
FT /id="PRO_0000356856"
FT REGION 365..436
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 365..387
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 388..407
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 408..423
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 86
FT /note="G->V: Unable to complement a ndufaf5 mutant strain."
FT /evidence="ECO:0000269|PubMed:23536703"
FT MUTAGEN 165
FT /note="L->F: Unable to complement a ndufaf5 mutant strain."
FT /evidence="ECO:0000269|PubMed:23536703"
FT MUTAGEN 235
FT /note="L->P: Unable to complement a ndufaf5 mutant strain."
FT /evidence="ECO:0000269|PubMed:23536703"
SQ SEQUENCE 436 AA; 50194 MW; 21C4C38D2297863A CRC64;
MLRTTFRKGF NLKCFSKDWN QTRQYSNYTK MTIFDTNVKT IQKNNTVTNV DDPKHYDYLM
NEVADRLADR ILDIKDIKCG NVLDFGSRNG ALFKYIQEKG AKIDKYYMVE SSKELLYRDD
NNVSQENEDD NNNNKVKPTK ILVNSLEDKI EGIEDQSLDL IISNLSLHWV NDLPGVFGGL
KRLLKPNGVF LASLFGEDTL MELKDSLYLA EIEREGGFSP HVSPFTKISD IGNILSKNRY
TLPTVDTEKI TINYDNMFVL MRDLQNMGEN NAILKRRNYT SKDTFLAASA IYKHLYGNED
NNSIPATFQI IYLIGWAPHE SQQKPLQRGS AKKHFSEISG TSSFGYKFDN DSSIPSILTN
ENNSVTLSQQ QQQQGIEPQQ SNDDSINEPF PKTDDFVIKR LDYHGNFHFE KQQQQQQQQD
QNKESSDEIN KNKDDK
