ID   NDUF5_RAT               Reviewed;         343 AA.
AC   B2GV71;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-JUN-2008, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Arginine-hydroxylase NDUFAF5, mitochondrial;
DE            EC=1.-.-.- {ECO:0000250|UniProtKB:Q5TEU4};
DE   AltName: Full=NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 {ECO:0000312|RGD:1309829};
DE   AltName: Full=Putative methyltransferase NDUFAF5 {ECO:0000305};
DE            EC=2.1.1.- {ECO:0000305};
DE   Flags: Precursor;
GN   Name=Ndufaf5 {ECO:0000312|RGD:1309829};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Ovary;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Arginine hydroxylase involved in the assembly of
CC       mitochondrial NADH:ubiquinone oxidoreductase complex (complex I, MT-
CC       ND1) at early stages. Acts by mediating hydroxylation of 'Arg-111' of
CC       NDUFS7. May also have methyltransferase activity.
CC       {ECO:0000250|UniProtKB:Q5TEU4}.
CC   -!- SUBUNIT: Interacts with NDUFAF8, leading to stabilize NDUFAF5.
CC       Interacts with NDUFS7. {ECO:0000250|UniProtKB:Q5TEU4}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:Q5TEU4}. Note=Peripherally localized on the
CC       matrix face of the mitochondrial inner membrane.
CC       {ECO:0000250|UniProtKB:Q5TEU4}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC       {ECO:0000305}.
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DR   EMBL; BC166551; AAI66551.1; -; mRNA.
DR   RefSeq; NP_001119843.1; NM_001126371.1.
DR   AlphaFoldDB; B2GV71; -.
DR   SMR; B2GV71; -.
DR   STRING; 10116.ENSRNOP00000006412; -.
DR   iPTMnet; B2GV71; -.
DR   PhosphoSitePlus; B2GV71; -.
DR   PaxDb; B2GV71; -.
DR   PeptideAtlas; B2GV71; -.
DR   PRIDE; B2GV71; -.
DR   Ensembl; ENSRNOT00000006412; ENSRNOP00000006412; ENSRNOG00000004784.
DR   GeneID; 296190; -.
DR   KEGG; rno:296190; -.
DR   UCSC; RGD:1309829; rat.
DR   CTD; 79133; -.
DR   RGD; 1309829; Ndufaf5.
DR   eggNOG; KOG2940; Eukaryota.
DR   GeneTree; ENSGT00390000014687; -.
DR   HOGENOM; CLU_046586_0_2_1; -.
DR   InParanoid; B2GV71; -.
DR   OMA; PSMWELM; -.
DR   OrthoDB; 691814at2759; -.
DR   PhylomeDB; B2GV71; -.
DR   TreeFam; TF315222; -.
DR   Reactome; R-RNO-6799198; Complex I biogenesis.
DR   PRO; PR:B2GV71; -.
DR   Proteomes; UP000002494; Chromosome 3.
DR   Bgee; ENSRNOG00000004784; Expressed in quadriceps femoris and 20 other tissues.
DR   Genevisible; B2GV71; RN.
DR   GO; GO:0031314; C:extrinsic component of mitochondrial inner membrane; ISO:RGD.
DR   GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR   GO; GO:0030961; P:peptidyl-arginine hydroxylation; ISS:UniProtKB.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR013216; Methyltransf_11.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF08241; Methyltransf_11; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Methyltransferase; Mitochondrion; Mitochondrion inner membrane;
KW   Oxidoreductase; Reference proteome; Transferase; Transit peptide.
FT   TRANSIT         1..29
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..343
FT                   /note="Arginine-hydroxylase NDUFAF5, mitochondrial"
FT                   /id="PRO_0000358879"
FT   REGION          18..40
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   343 AA;  38229 MW;  5FFEE0F26E92A44D CRC64;
     MLRRVVLSRL YARLGGPAVS AGRGGRRGVA SSVPPSGSTS PRALNIFDRE LKRKQKNWAA
     RQPEPMKFDY LKEEIGSRIA DRVYDIARDF PLALDIGCGR GYIAQHLNKE TVGKIFQTDI
     AEHALKNSIE TDIPTVNILA DEEFLPFPEN TFDLVVSSLS LHWVNDLPRA LEQIHYVLKP
     DGVFVGAMFG GDTLYELRCS LQLAETEREG GFSPHISPFT AVNDLGHLLG RAGFNTLTVD
     TDEIQVNYPG MFELMEDLKG MGESNCSWNR KALLHRDTML AAAAVYREMY SNEDGSIPAT
     YQIYHMIGWK YHDSQARPAE RGSATVSFGD LARLNDTMSQ GKK