ID   NDUS2_CAFRO             Reviewed;         398 AA.
AC   Q9TAJ7;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=NADH-ubiquinone oxidoreductase 49 kDa subunit;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 7;
GN   Name=NAD7;
OS   Cafeteria roenbergensis (Marine flagellate).
OG   Mitochondrion.
OC   Eukaryota; Sar; Stramenopiles; Bigyra; Opalozoa; Bicosoecida;
OC   Cafeteriaceae; Cafeteria.
OX   NCBI_TaxID=33653;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Burger G.;
RT   "The mitochondrial genome of Cafeteria roenbergensis.";
RL   Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). Component of the iron-sulfur (IP) fragment of the enzyme.
CC       Component of the iron-sulfur (IP) fragment of the enzyme (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion.
CC   -!- SIMILARITY: Belongs to the complex I 49 kDa subunit family.
CC       {ECO:0000305}.
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DR   EMBL; AF193903; AAF05789.1; -; Genomic_DNA.
DR   RefSeq; NP_051138.1; NC_000946.1.
DR   AlphaFoldDB; Q9TAJ7; -.
DR   SMR; Q9TAJ7; -.
DR   GeneID; 800841; -.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0048038; F:quinone binding; IEA:InterPro.
DR   Gene3D; 1.10.645.10; -; 1.
DR   HAMAP; MF_01358; NDH1_NuoD; 1.
DR   InterPro; IPR001135; NADH_Q_OxRdtase_suD.
DR   InterPro; IPR014029; NADH_UbQ_OxRdtase_49kDa_CS.
DR   InterPro; IPR022885; NDH1_su_D/H.
DR   InterPro; IPR029014; NiFe-Hase_large.
DR   PANTHER; PTHR11993; PTHR11993; 1.
DR   Pfam; PF00346; Complex1_49kDa; 1.
DR   SUPFAM; SSF56762; SSF56762; 1.
DR   TIGRFAMs; TIGR01962; NuoD; 1.
DR   PROSITE; PS00535; COMPLEX1_49K; 1.
PE   3: Inferred from homology;
KW   Electron transport; Mitochondrion; NAD; Oxidoreductase; Respiratory chain;
KW   Translocase; Transport; Ubiquinone.
FT   CHAIN           1..398
FT                   /note="NADH-ubiquinone oxidoreductase 49 kDa subunit"
FT                   /id="PRO_0000118588"
SQ   SEQUENCE   398 AA;  45813 MW;  1BDDAF669C91404E CRC64;
     MFIKRKLKET EIKDFTLNFG PQHPAAHGVL RLILELNGET IKNADPHIGL LHRGTEKLIE
     NRNYLQALPY FDRLDYVSMM VQEHAYSLAV ERLYGINIPQ RAQWIRVLFS EITRILNHLL
     AIGCHSMDVG AMTPLLWGFE EREKLMEFYE RVSGARMHAS YIRPGGVAQD LPSGFVEDVY
     SFILDFSVRL DEIEELLTNN RIWKQRLVNV GIVTAKQAIQ NGFSGVLLRS TGIPWDLRRS
     QPYEIYDKVP FRIPVGTRGD CYDRYLIRMQ EMRQSLRIMM ACLKYLPGGS IKSDDKKFVP
     PLRWEMKNSM ESVIHHFKYF TEGFKVPAGQ TYAAVEAPKG EMGVFLISDG SNQPFRCKIK
     APGFFHLQNL PEMTKHHMIA DVVTIIGTQD IVFGEVDR