ID   NDUS2_RECAM             Reviewed;         396 AA.
AC   O21270;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=NADH-ubiquinone oxidoreductase 49 kDa subunit;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 7;
GN   Name=NAD7;
OS   Reclinomonas americana.
OG   Mitochondrion.
OC   Eukaryota; Discoba; Jakobida; Histionina; Histionidae; Reclinomonas.
OX   NCBI_TaxID=48483;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 50394;
RX   PubMed=9168110; DOI=10.1038/387493a0;
RA   Lang B.F., Burger G., O'Kelly C.J., Cedergren R., Golding G.B., Lemieux C.,
RA   Sankoff D., Turmel M., Gray M.W.;
RT   "An ancestral mitochondrial DNA resembling a eubacterial genome in
RT   miniature.";
RL   Nature 387:493-497(1997).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). Component of the iron-sulfur (IP) fragment of the enzyme.
CC       Component of the iron-sulfur (IP) fragment of the enzyme.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion.
CC   -!- SIMILARITY: Belongs to the complex I 49 kDa subunit family.
CC       {ECO:0000305}.
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DR   EMBL; AF007261; AAD11897.1; -; Genomic_DNA.
DR   PIR; S78164; S78164.
DR   RefSeq; NP_044782.1; NC_001823.1.
DR   AlphaFoldDB; O21270; -.
DR   SMR; O21270; -.
DR   GeneID; 801113; -.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0048038; F:quinone binding; IEA:InterPro.
DR   Gene3D; 1.10.645.10; -; 1.
DR   HAMAP; MF_01358; NDH1_NuoD; 1.
DR   InterPro; IPR001135; NADH_Q_OxRdtase_suD.
DR   InterPro; IPR014029; NADH_UbQ_OxRdtase_49kDa_CS.
DR   InterPro; IPR022885; NDH1_su_D/H.
DR   InterPro; IPR029014; NiFe-Hase_large.
DR   PANTHER; PTHR11993; PTHR11993; 1.
DR   Pfam; PF00346; Complex1_49kDa; 1.
DR   SUPFAM; SSF56762; SSF56762; 1.
DR   TIGRFAMs; TIGR01962; NuoD; 1.
DR   PROSITE; PS00535; COMPLEX1_49K; 1.
PE   3: Inferred from homology;
KW   Electron transport; Mitochondrion; NAD; Oxidoreductase; Respiratory chain;
KW   Translocase; Transport; Ubiquinone.
FT   CHAIN           1..396
FT                   /note="NADH-ubiquinone oxidoreductase 49 kDa subunit"
FT                   /id="PRO_0000118593"
SQ   SEQUENCE   396 AA;  44993 MW;  252B68E29305F096 CRC64;
     MQQKALTKKL KNFTMNFGPQ HPAAHGVLRL VLELNGEVVN RADPHIGLLH RGTEKLIEYK
     NYLQALPYFD RLDYVSMMAQ EHAYSLAVEK LLGCEVPKRA QYIRVLFCEI TRILNHLMAV
     TTHALDVGAM TPFLWGFEER EKLMEFYERV SGARMHAAYI RPGGVSQDMP MGLSEDIYKF
     AKQFGSRIDE IEDVLSSNRI WKQRLVDIGT VSAEEALDWG FSGVMLRGSG IAWDLRKTQP
     YDVYDELEFD IPVGTKGDCY DRYLIRVEEM RQSLKLIMQC LNKMPTGVIK VDDKKISPPS
     RVDMKDSMEA LIHHFKLYTE GYSVPIGETY TAVEAPKGEF GVYLVSDGSS KPYRCKIKAP
     GFSHLQGLNF MSKGHMIADV VTIIGTQDIV FGEVDR