ID   NDUS3_MESAU             Reviewed;          94 AA.
AC   P86217;
DT   18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT   18-MAY-2010, sequence version 1.
DT   25-MAY-2022, entry version 31.
DE   RecName: Full=NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial {ECO:0000250|UniProtKB:P23709};
DE            EC=7.1.1.2 {ECO:0000250|UniProtKB:O75489};
DE   AltName: Full=Complex I-30kD {ECO:0000250|UniProtKB:P23709};
DE            Short=CI-30kD {ECO:0000250|UniProtKB:P23709};
DE   AltName: Full=NADH-ubiquinone oxidoreductase 30 kDa subunit {ECO:0000250|UniProtKB:P23709};
DE   Flags: Fragments;
GN   Name=NDUFS3 {ECO:0000250|UniProtKB:P23709};
OS   Mesocricetus auratus (Golden hamster).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea;
OC   Cricetidae; Cricetinae; Mesocricetus.
OX   NCBI_TaxID=10036;
RN   [1]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=20400973; DOI=10.1038/aja.2010.19;
RA   Kameshwari D.B., Bhande S., Sundaram C.S., Kota V., Siva A.B., Shivaji S.;
RT   "Glucose-regulated protein precursor (GRP78) and tumor rejection antigen
RT   (GP96) are unique to hamster caput epididymal spermatozoa.";
RL   Asian J. Androl. 12:344-355(2010).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) which catalyzes electron transfer from
CC       NADH through the respiratory chain, using ubiquinone as an electron
CC       acceptor (By similarity). Essential for the catalytic activity and
CC       assembly of complex I (By similarity). {ECO:0000250|UniProtKB:O75489}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC         Evidence={ECO:0000250|UniProtKB:O75489};
CC   -!- SUBUNIT: Core subunit of respiratory chain NADH dehydrogenase (Complex
CC       I) which is composed of 45 different subunits (By similarity).
CC       Interacts with NDUFAF3 (By similarity). Interacts with RAB5IF (By
CC       similarity). Found in subcomplexes containing subunits NDUFS2, MT-ND1
CC       and NDUFA13 (By similarity). {ECO:0000250|UniProtKB:O75489}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:O75489}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:O75489}; Matrix side
CC       {ECO:0000250|UniProtKB:O75489}.
CC   -!- SIMILARITY: Belongs to the complex I 30 kDa subunit family.
CC       {ECO:0000255}.
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DR   AlphaFoldDB; P86217; -.
DR   Proteomes; UP000189706; Unplaced.
DR   GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; ISS:UniProtKB.
DR   GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; ISS:UniProtKB.
DR   GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR   InterPro; IPR037232; NADH_quin_OxRdtase_su_C/D-like.
DR   InterPro; IPR001268; NADH_UbQ_OxRdtase_30kDa_su.
DR   Pfam; PF00329; Complex1_30kDa; 1.
DR   SUPFAM; SSF143243; SSF143243; 1.
PE   1: Evidence at protein level;
KW   Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   NAD; Oxidoreductase; Reference proteome; Respiratory chain; Translocase;
KW   Transport; Ubiquinone.
FT   CHAIN           <1..>94
FT                   /note="NADH dehydrogenase [ubiquinone] iron-sulfur protein
FT                   3, mitochondrial"
FT                   /id="PRO_0000394305"
FT   NON_CONS        14..15
FT                   /evidence="ECO:0000305"
FT   NON_CONS        28..29
FT                   /evidence="ECO:0000305"
FT   NON_CONS        45..46
FT                   /evidence="ECO:0000305"
FT   NON_CONS        59..60
FT                   /evidence="ECO:0000305"
FT   NON_TER         1
FT   NON_TER         94
SQ   SEQUENCE   94 AA;  11107 MW;  13BC4321646C6418 CRC64;
     VCCRGLLGAA SVGRQNRFEI VYNLLSLREV WDMFGVFFFN HPDLRILTDY GFEGHPFRKY
     DDEVKRVVAE PVELAQEFRK FDLNSPWEAF PAYR