NDUS3_SOLTU
ID NDUS3_SOLTU Reviewed; 190 AA.
AC P80261;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2003, sequence version 3.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=NADH dehydrogenase [ubiquinone] iron-sulfur protein 3;
DE EC=7.1.1.2;
DE AltName: Full=Complex I-27kD;
DE Short=CI-27kD;
DE AltName: Full=NADH dehydrogenase subunit 9;
DE AltName: Full=NADH-ubiquinone oxidoreductase 27 kDa subunit;
GN Name=NAD9;
OS Solanum tuberosum (Potato).
OG Mitochondrion.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
OX NCBI_TaxID=4113;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, AND RNA
RP EDITING.
RC TISSUE=Tuber;
RX PubMed=8078764; DOI=10.1093/nar/22.16.3304;
RA Grohmann L., Thieck O., Herz U., Schroeder W., Brennicke A.;
RT "Translation of nad9 mRNAs in mitochondria from Solanum tuberosum is
RT restricted to completely edited transcripts.";
RL Nucleic Acids Res. 22:3304-3311(1994).
RN [2]
RP PROTEIN SEQUENCE OF 1-17.
RC STRAIN=cv. Bintje; TISSUE=Tuber;
RX PubMed=8294484; DOI=10.1016/s0021-9258(17)42163-6;
RA Herz U., Schroeder W., Liddell A., Leaver C.J., Brennicke A., Grohmann L.;
RT "Purification of the NADH:ubiquinone oxidoreductase (complex I) of the
RT respiratory chain from the inner mitochondrial membrane of Solanum
RT tuberosum.";
RL J. Biol. Chem. 269:2263-2269(1994).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBUNIT: Complex I is composed of about 45 different subunits. This is
CC a component of the iron-sulfur (IP) fragment of the enzyme (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Peripheral membrane
CC protein; Matrix side.
CC -!- RNA EDITING: Modified_positions=31 {ECO:0000269|PubMed:8078764}, 56
CC {ECO:0000269|PubMed:8078764}, 100 {ECO:0000269|PubMed:8078764}, 110
CC {ECO:0000269|PubMed:8078764}, 122 {ECO:0000269|PubMed:8078764}, 133
CC {ECO:0000269|PubMed:8078764}, 147 {ECO:0000269|PubMed:8078764};
CC -!- SIMILARITY: Belongs to the complex I 30 kDa subunit family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X79774; CAA56168.1; ALT_SEQ; Genomic_DNA.
DR PIR; S48062; S48062.
DR AlphaFoldDB; P80261; -.
DR SMR; P80261; -.
DR PRIDE; P80261; -.
DR InParanoid; P80261; -.
DR Proteomes; UP000011115; Unassembled WGS sequence.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IBA:GO_Central.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR Gene3D; 3.30.460.80; -; 1.
DR HAMAP; MF_01357; NDH1_NuoC; 1.
DR InterPro; IPR010218; NADH_DH_suC.
DR InterPro; IPR037232; NADH_quin_OxRdtase_su_C/D-like.
DR InterPro; IPR001268; NADH_UbQ_OxRdtase_30kDa_su.
DR InterPro; IPR020396; NADH_UbQ_OxRdtase_CS.
DR Pfam; PF00329; Complex1_30kDa; 1.
DR SUPFAM; SSF143243; SSF143243; 1.
DR TIGRFAMs; TIGR01961; NuoC_fam; 1.
DR PROSITE; PS00542; COMPLEX1_30K; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Electron transport; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; NAD; Oxidoreductase; Reference proteome;
KW Respiratory chain; RNA editing; Translocase; Transport; Ubiquinone.
FT CHAIN 1..190
FT /note="NADH dehydrogenase [ubiquinone] iron-sulfur protein
FT 3"
FT /id="PRO_0000118643"
SQ SEQUENCE 190 AA; 22883 MW; A150CF461B493A53 CRC64;
MDNQFIFKYS WETLPKKWVK KMERSEHGNR FDTNTDYLFQ LLCFMKLHTY TRVQVLIDIC
GVDYPSRKQR FEVVYNLLSI RYNSRIRVQT SADEVTRISS VVSLFPSAGW WEREVWDMFG
VFSINHPDLR RILTDYGFEG HPLRKDFPLS GYVEVRYDDP EKRVVSEPIE MTQEFRYFDF
ASPWEQRSDG