ID   NDUS4_NEUCR             Reviewed;         218 AA.
AC   P25711; P40916; Q7RVF0;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 2.
DT   25-MAY-2022, entry version 141.
DE   RecName: Full=NADH-ubiquinone oxidoreductase 21 kDa subunit, mitochondrial;
DE   AltName: Full=Complex I-21kD;
DE            Short=CI-21kD;
DE   Flags: Precursor;
GN   Name=nuo-21; Synonyms=nuo-18; ORFNames=NCU05221;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 12-218.
RX   PubMed=7947902; DOI=10.1016/0005-2728(94)90034-5;
RA   Azevedo J.E., Duarte M., Belo J.A., Werner S., Videira A.;
RT   "Complementary DNA sequences of the 24 kDa and 21 kDa subunits of complex I
RT   from Neurospora.";
RL   Biochim. Biophys. Acta 1188:159-161(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 34-59.
RC   STRAIN=74-ORS-6a / FGSC 4200;
RX   PubMed=1830490; DOI=10.1016/0167-4781(91)90182-l;
RA   Weidner U., Sackmann U., Nehls U., Weiss H.;
RT   "Primary structure of the nuclear-encoded 18.3 kDa subunit of NADH:
RT   ubiquinone reductase (complex I) from Neurospora crassa mitochondria.";
RL   Biochim. Biophys. Acta 1089:391-392(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC       chain NADH dehydrogenase (Complex I), that is believed not to be
CC       involved in catalysis. Complex I functions in the transfer of electrons
CC       from NADH to the respiratory chain. The immediate electron acceptor for
CC       the enzyme is believed to be ubiquinone.
CC   -!- SUBUNIT: Complex I is composed of about 40 different subunits. This is
CC       a component of the iron-sulfur (IP) fragment of the enzyme.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane.
CC   -!- SIMILARITY: Belongs to the complex I NDUFS4 subunit family.
CC       {ECO:0000305}.
CC   -!- CAUTION: It is uncertain whether Met-1 or Met-14 is the initiator.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA39675.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; X78082; CAA54989.1; -; mRNA.
DR   EMBL; X56226; CAA39675.1; ALT_FRAME; mRNA.
DR   EMBL; CM002239; EAA32645.1; -; Genomic_DNA.
DR   PIR; S17192; S17192.
DR   RefSeq; XP_961881.1; XM_956788.3.
DR   AlphaFoldDB; P25711; -.
DR   SMR; P25711; -.
DR   STRING; 5141.EFNCRP00000004969; -.
DR   TCDB; 3.D.1.6.2; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR   EnsemblFungi; EAA32645; EAA32645; NCU05221.
DR   GeneID; 3878036; -.
DR   KEGG; ncr:NCU05221; -.
DR   VEuPathDB; FungiDB:NCU05221; -.
DR   HOGENOM; CLU_077196_0_0_1; -.
DR   InParanoid; P25711; -.
DR   OMA; FMNGHRI; -.
DR   Proteomes; UP000001805; Chromosome 4, Linkage Group IV.
DR   GO; GO:0005747; C:mitochondrial respiratory chain complex I; IBA:GO_Central.
DR   GO; GO:0022900; P:electron transport chain; IEA:InterPro.
DR   Gene3D; 3.30.160.190; -; 1.
DR   InterPro; IPR006885; NADH_UbQ_FeS_4_mit.
DR   InterPro; IPR038532; NDUFS4-like_sf.
DR   PANTHER; PTHR12219; PTHR12219; 1.
DR   Pfam; PF04800; ETC_C1_NDUFA4; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Electron transport; Membrane; Mitochondrion;
KW   Mitochondrion inner membrane; Reference proteome; Respiratory chain;
KW   Transit peptide; Transport.
FT   TRANSIT         1..33
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000269|PubMed:1830490"
FT   CHAIN           34..218
FT                   /note="NADH-ubiquinone oxidoreductase 21 kDa subunit,
FT                   mitochondrial"
FT                   /id="PRO_0000020036"
FT   REGION          31..74
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   218 AA;  24474 MW;  5BE7AAF20AA92AA7 CRC64;
     MSALRITTAS AARMLRTSNA MMPSVMGAAQ RRALSDSAEP ARVPSVESAR VPEKLAKEDS
     PLATPKRNSP DYNVPIDKAT STWTPVPKHI QNGSEEGILP AAVVSGAPME LQARTVRIYL
     PSKPATQSSN SRVLWRMDWD VLEKGHRWEN ELMGWQSSGD FVQGTHLTFR TKEEAIQFAE
     KQGYEYFVQE PNQRHFTPKA YANNFLYSPK KLKIVRTK