ID   NDUS7_PARTE             Reviewed;         156 AA.
AC   P15602; Q35365;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=NADH-ubiquinone oxidoreductase 20 kDa subunit;
DE            EC=7.1.1.2;
GN   Name=NAD10; Synonyms=PSBG;
OS   Paramecium tetraurelia.
OG   Mitochondrion.
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC   Oligohymenophorea; Peniculida; Parameciidae; Paramecium.
OX   NCBI_TaxID=5888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Stock 51;
RX   PubMed=2308823; DOI=10.1093/nar/18.1.173;
RA   Pritchard A.E., Seilhamer J.J., Mahalingam R., Sable C.L., Venuti S.E.,
RA   Cummings D.J.;
RT   "Nucleotide sequence of the mitochondrial genome of Paramecium.";
RL   Nucleic Acids Res. 18:173-180(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Stock 51;
RX   PubMed=2670676; DOI=10.1016/0378-1119(89)90320-x;
RA   Pritchard A.E., Venuti S.E., Ghalambor M.A., Sable C.L., Cummings D.J.;
RT   "An unusual region of Paramecium mitochondrial DNA containing chloroplast-
RT   like genes.";
RL   Gene 78:121-134(1989).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000305};
CC       Note=Binds 1 [4Fe-4S] cluster. {ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion.
CC   -!- SIMILARITY: Belongs to the complex I 20 kDa subunit family.
CC       {ECO:0000305}.
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DR   EMBL; X15917; CAA34045.1; -; Genomic_DNA.
DR   EMBL; M26930; AAA79257.1; -; Genomic_DNA.
DR   PIR; S07736; F2PPG.
DR   AlphaFoldDB; P15602; -.
DR   SMR; P15602; -.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0048038; F:quinone binding; IEA:InterPro.
DR   HAMAP; MF_01356; NDH1_NuoB; 1.
DR   InterPro; IPR006137; NADH_UbQ_OxRdtase-like_20kDa.
DR   InterPro; IPR006138; NADH_UQ_OxRdtase_20Kd_su.
DR   Pfam; PF01058; Oxidored_q6; 1.
DR   TIGRFAMs; TIGR01957; nuoB_fam; 1.
DR   PROSITE; PS01150; COMPLEX1_20K; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Electron transport; Iron; Iron-sulfur; Metal-binding;
KW   Mitochondrion; NAD; Oxidoreductase; Respiratory chain; Translocase;
KW   Transport.
FT   CHAIN           1..156
FT                   /note="NADH-ubiquinone oxidoreductase 20 kDa subunit"
FT                   /id="PRO_0000118739"
FT   BINDING         33
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000255"
FT   BINDING         34
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000255"
FT   BINDING         98
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000255"
FT   BINDING         128
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        123..127
FT                   /note="MLCPR -> IFVPG (in Ref. 2; AAA79257)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   156 AA;  17454 MW;  B056E34343D75C2F CRC64;
     MILKADFLKL SANNLISWAR QGSFWPLTFG LACCALEMMH ATVSRYDFDR FGVIFRATPR
     QADLIIVAGT VTNKMAPALR RLYDQTADPK WVLSMGSCAN GGGYYHYSYA VVKGCDKIIP
     VDMLCPRCPP TAEALFFGVL QLQKTLMKTI NEKKVF