ID   NDUS7_RECAM             Reviewed;         182 AA.
AC   O21272;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=NADH-ubiquinone oxidoreductase 20 kDa subunit;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 10;
GN   Name=NAD10;
OS   Reclinomonas americana.
OG   Mitochondrion.
OC   Eukaryota; Discoba; Jakobida; Histionina; Histionidae; Reclinomonas.
OX   NCBI_TaxID=48483;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 50394;
RX   PubMed=9168110; DOI=10.1038/387493a0;
RA   Lang B.F., Burger G., O'Kelly C.J., Cedergren R., Golding G.B., Lemieux C.,
RA   Sankoff D., Turmel M., Gray M.W.;
RT   "An ancestral mitochondrial DNA resembling a eubacterial genome in
RT   miniature.";
RL   Nature 387:493-497(1997).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000305};
CC       Note=Binds 1 [4Fe-4S] cluster. {ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion.
CC   -!- SIMILARITY: Belongs to the complex I 20 kDa subunit family.
CC       {ECO:0000305}.
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DR   EMBL; AF007261; AAD11899.1; -; Genomic_DNA.
DR   PIR; S78166; S78166.
DR   RefSeq; NP_044784.1; NC_001823.1.
DR   AlphaFoldDB; O21272; -.
DR   SMR; O21272; -.
DR   GeneID; 801085; -.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0048038; F:quinone binding; IEA:InterPro.
DR   HAMAP; MF_01356; NDH1_NuoB; 1.
DR   InterPro; IPR006137; NADH_UbQ_OxRdtase-like_20kDa.
DR   InterPro; IPR006138; NADH_UQ_OxRdtase_20Kd_su.
DR   Pfam; PF01058; Oxidored_q6; 1.
DR   TIGRFAMs; TIGR01957; nuoB_fam; 1.
DR   PROSITE; PS01150; COMPLEX1_20K; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Electron transport; Iron; Iron-sulfur; Metal-binding;
KW   Mitochondrion; NAD; Oxidoreductase; Respiratory chain; Translocase;
KW   Transport.
FT   CHAIN           1..182
FT                   /note="NADH-ubiquinone oxidoreductase 20 kDa subunit"
FT                   /id="PRO_0000118740"
FT   BINDING         57
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000255"
FT   BINDING         58
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000255"
FT   BINDING         122
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000255"
FT   BINDING         152
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   182 AA;  20925 MW;  0B4663AB65303D48 CRC64;
     MEERCFRLGI KTNYKKEIFM ILNNKQNKTE FIVSKMDELV NWARKGSLWP MTFGLACCAV
     EMMHSAASRY DLDRFGIIFR PSPRQSDVMI VAGTLTNKMA PALRKVYDQM SEPRWVVSMG
     SCANGGGYYH YSYSVVRGCD RIVPVDIYVP GCPPTAEALL YGLLQLQKKI KRSRKTLYWL
     QK