NEAP1_ARATH
ID NEAP1_ARATH Reviewed; 336 AA.
AC Q9M9L3; Q56Y98; Q9ZRC5;
DT 27-SEP-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Nuclear envelope-associated protein 1 {ECO:0000303|PubMed:27630107};
DE Short=AtNEAP1 {ECO:0000303|PubMed:27630107};
DE AltName: Full=Plant IF-like protein {ECO:0000312|EMBL:AAD09217.1};
GN Name=NEAP1 {ECO:0000303|PubMed:27630107};
GN OrderedLocusNames=At3g05830 {ECO:0000312|Araport:AT3G05830};
GN ORFNames=F10A16.12 {ECO:0000312|EMBL:AAF26085.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Landsberg erecta;
RA Colter M.E., Saunders M.J.;
RT "Arabidopsis thaliana intermediate filament-related plant cDNA clones
RT isolated by IFA screening of a flower expression library.";
RL Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-195.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, NOMENCLATURE, SUBCELLULAR LOCATION, SUBUNIT, INTERACTION WITH
RP NEAP2; NEAP3; SUN1; SUN2 AND BZIP18, AND DISRUPTION PHENOTYPE.
RX PubMed=27630107; DOI=10.1093/jxb/erw332;
RA Pawar V., Poulet A., Detourne G., Tatout C., Vanrobays E., Evans D.E.,
RA Graumann K.;
RT "A novel family of plant nuclear envelope-associated proteins.";
RL J. Exp. Bot. 67:5699-5710(2016).
CC -!- SUBUNIT: Forms heteromers with NEAP2 and NEAP3. Interacts with SUN1;
CC SUN2 and bZIP18. {ECO:0000269|PubMed:27630107}.
CC -!- SUBCELLULAR LOCATION: Nucleus inner membrane
CC {ECO:0000269|PubMed:27630107}; Single-pass membrane protein
CC {ECO:0000255}. Nucleus, nucleoplasm {ECO:0000269|PubMed:27630107}.
CC Note=Colocalized with bZIP18 in the nucleoplasm.
CC {ECO:0000269|PubMed:27630107}.
CC -!- DISRUPTION PHENOTYPE: Double mutants NEAP1-NEAP3 display reduced
CC primary root growth, and altered nuclear morphology and chromatin
CC structure. {ECO:0000269|PubMed:27630107}.
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DR EMBL; U72393; AAD09217.1; -; mRNA.
DR EMBL; AC012393; AAF26085.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74301.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM65895.1; -; Genomic_DNA.
DR EMBL; AY084526; AAM61094.1; -; mRNA.
DR EMBL; AK221425; BAD94422.1; -; mRNA.
DR RefSeq; NP_001327832.1; NM_001337615.1.
DR RefSeq; NP_566262.1; NM_111457.2.
DR AlphaFoldDB; Q9M9L3; -.
DR SMR; Q9M9L3; -.
DR STRING; 3702.AT3G05830.2; -.
DR PRIDE; Q9M9L3; -.
DR EnsemblPlants; AT3G05830.1; AT3G05830.1; AT3G05830.
DR EnsemblPlants; AT3G05830.3; AT3G05830.3; AT3G05830.
DR GeneID; 819752; -.
DR Gramene; AT3G05830.1; AT3G05830.1; AT3G05830.
DR Gramene; AT3G05830.3; AT3G05830.3; AT3G05830.
DR KEGG; ath:AT3G05830; -.
DR Araport; AT3G05830; -.
DR OMA; KLECTLK; -.
DR PhylomeDB; Q9M9L3; -.
DR PRO; PR:Q9M9L3; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M9L3; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005637; C:nuclear inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
PE 1: Evidence at protein level;
KW Coiled coil; Membrane; Nucleus; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..336
FT /note="Nuclear envelope-associated protein 1"
FT /id="PRO_0000441686"
FT TRANSMEM 313..330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT COILED 125..261
FT /evidence="ECO:0000255"
FT MOTIF 240..261
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000305|PubMed:27630107"
FT CONFLICT 1..3
FT /note="MSY -> MAEFC (in Ref. 1; AAD09217)"
FT /evidence="ECO:0000305"
FT CONFLICT 161
FT /note="S -> G (in Ref. 5; BAD94422)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 336 AA; 39578 MW; 370B2A51687A7017 CRC64;
MSYSEKTTVD PLLRDLDEKK ESFRRNVVSL ATELKQVRGR LVSQEQSFLK ETITRKEAEK
RGKNMEMEIC KLQKRLEERN CQLEASASAA DKFIKELEEF RLKLDTTKQT AEASADSAQS
TKIQCSMLKQ QLDDKTRSLR EQEDRMTQLG HQLDDLQRGL SLRECSEKQL REEVRRIERE
VTEAIAKAGI GGMDSELQKL LEDVSPMKFE RMNRLVEVKD EEITKLKDEI RLMSGQWKHK
TKELESQLEK QRRTDQDLKK KVLKLEFCLQ EARSQTRKLQ RKGERRDMEI KEIRDLISEK
QNLNNESWDK QKFWDNSGFK IVVSMSMLML VVVSKR
