NEAP2_ARATH
ID NEAP2_ARATH Reviewed; 335 AA.
AC F4K1B4; Q5PP55;
DT 27-SEP-2017, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Nuclear envelope-associated protein 2 {ECO:0000303|PubMed:27630107};
DE Short=AtNEAP2 {ECO:0000303|PubMed:27630107};
GN Name=NEAP2 {ECO:0000303|PubMed:27630107};
GN OrderedLocusNames=At5g26770 {ECO:0000312|Araport:AT5G26770};
GN ORFNames=F2P16.30 {ECO:0000312|EMBL:AF007270};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, NOMENCLATURE, SUBCELLULAR LOCATION, SUBUNIT, AND INTERACTION
RP WITH NEAP1; NEAP3; SUN1 AND SUN2.
RX PubMed=27630107; DOI=10.1093/jxb/erw332;
RA Pawar V., Poulet A., Detourne G., Tatout C., Vanrobays E., Evans D.E.,
RA Graumann K.;
RT "A novel family of plant nuclear envelope-associated proteins.";
RL J. Exp. Bot. 67:5699-5710(2016).
CC -!- SUBUNIT: Forms homomers and heteromers with NEAP1 and NEAP3. Interacts
CC with SUN1 and SUN2. {ECO:0000269|PubMed:27630107}.
CC -!- SUBCELLULAR LOCATION: Nucleus inner membrane
CC {ECO:0000269|PubMed:27630107}; Single-pass membrane protein
CC {ECO:0000255}. Nucleus, nucleoplasm {ECO:0000269|PubMed:27630107}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4K1B4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4K1B4-2; Sequence=VSP_059089;
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DR EMBL; AF007270; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED93599.1; -; Genomic_DNA.
DR EMBL; CP002688; AED93600.1; -; Genomic_DNA.
DR EMBL; CP002688; AED93601.1; -; Genomic_DNA.
DR EMBL; BX830163; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BT020242; AAV74236.1; -; mRNA.
DR EMBL; BT020518; AAW39019.1; -; mRNA.
DR RefSeq; NP_001031946.1; NM_001036869.2. [F4K1B4-2]
DR RefSeq; NP_568487.1; NM_122559.2. [F4K1B4-1]
DR RefSeq; NP_974840.1; NM_203111.2. [F4K1B4-1]
DR AlphaFoldDB; F4K1B4; -.
DR SMR; F4K1B4; -.
DR STRING; 3702.AT5G26770.1; -.
DR PaxDb; F4K1B4; -.
DR PRIDE; F4K1B4; -.
DR EnsemblPlants; AT5G26770.1; AT5G26770.1; AT5G26770. [F4K1B4-1]
DR EnsemblPlants; AT5G26770.2; AT5G26770.2; AT5G26770. [F4K1B4-1]
DR EnsemblPlants; AT5G26770.3; AT5G26770.3; AT5G26770. [F4K1B4-2]
DR GeneID; 832735; -.
DR Gramene; AT5G26770.1; AT5G26770.1; AT5G26770. [F4K1B4-1]
DR Gramene; AT5G26770.2; AT5G26770.2; AT5G26770. [F4K1B4-1]
DR Gramene; AT5G26770.3; AT5G26770.3; AT5G26770. [F4K1B4-2]
DR KEGG; ath:AT5G26770; -.
DR Araport; AT5G26770; -.
DR TAIR; locus:2148448; AT5G26770.
DR eggNOG; ENOG502QUNG; Eukaryota.
DR HOGENOM; CLU_077822_0_0_1; -.
DR InParanoid; F4K1B4; -.
DR OMA; TGCTEQY; -.
DR PRO; PR:F4K1B4; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; F4K1B4; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005635; C:nuclear envelope; IDA:TAIR.
DR GO; GO:0005637; C:nuclear inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IDA:TAIR.
DR GO; GO:0043621; F:protein self-association; IPI:UniProtKB.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Membrane; Nucleus; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..335
FT /note="Nuclear envelope-associated protein 2"
FT /id="PRO_0000441687"
FT TRANSMEM 312..329
FT /note="Helical"
FT /evidence="ECO:0000255"
FT COILED 55..85
FT /evidence="ECO:0000255"
FT COILED 125..260
FT /evidence="ECO:0000255"
FT MOTIF 239..260
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000305|PubMed:27630107"
FT VAR_SEQ 261..280
FT /note="Missing (in isoform 2)"
FT /id="VSP_059089"
FT CONFLICT 53
FT /note="F -> L (in Ref. 3; BX830163)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 335 AA; 38675 MW; D2333C99D640FAEE CRC64;
MSDSVKTTVD PLLKDLDGKK ESFRRNVVSM AAELKQVRGR LVSQEQFFVK ESFCRKEAEK
KAKNMEMEIC KLQKKLEDRN CELVASTSAA EKFLEEVDDL RSQLALTKDI AETSAASAQS
AQLQCSVLTE QLDDKTRSLR EHEDRVTHLG HQLDNLQRDL KTRECSQKQL REEVMRIERE
ITEAVAKSGK GTECELRKLL EEVSPKNFER MNMLLAVKDE EIAKLKDDVK LMSAHWKLKT
KELESQLERQ RRADQELKKK VLKLEFCLQE ARSQTRKLQR AGERRDKAIK ELSDQITGKQ
LNESVSGEKQ NFWDTSGFKI VVSMSMLILV IISKR
