NEAP3_ARATH
ID NEAP3_ARATH Reviewed; 336 AA.
AC Q4PT37; O80530;
DT 27-SEP-2017, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Nuclear envelope-associated protein 3 {ECO:0000303|PubMed:27630107};
DE Short=AtNEAP3 {ECO:0000303|PubMed:27630107};
GN Name=NEAP3 {ECO:0000303|PubMed:27630107};
GN OrderedLocusNames=At1g09470 {ECO:0000312|Araport:AT1G09470};
GN ORFNames=F14J9.13 {ECO:0000312|EMBL:AAC33207.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY, NOMENCLATURE, SUBCELLULAR LOCATION, SUBUNIT, INTERACTION WITH
RP NEAP1; NEAP2; SUN1 AND SUN2, AND DISRUPTION PHENOTYPE.
RX PubMed=27630107; DOI=10.1093/jxb/erw332;
RA Pawar V., Poulet A., Detourne G., Tatout C., Vanrobays E., Evans D.E.,
RA Graumann K.;
RT "A novel family of plant nuclear envelope-associated proteins.";
RL J. Exp. Bot. 67:5699-5710(2016).
CC -!- SUBUNIT: Forms homomers and heteromers with NEAP1 and NEAP2. Interacts
CC with SUN1 and SUN2. {ECO:0000269|PubMed:27630107}.
CC -!- SUBCELLULAR LOCATION: Nucleus inner membrane
CC {ECO:0000269|PubMed:27630107}; Single-pass membrane protein
CC {ECO:0000255}. Nucleus, nucleoplasm {ECO:0000269|PubMed:27630107}.
CC -!- DISRUPTION PHENOTYPE: Double mutants NEAP1-NEAP3 display reduced
CC primary root growth, and altered nuclear morphology and chromatin
CC structure. {ECO:0000269|PubMed:27630107}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC33207.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC003970; AAC33207.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28446.1; -; Genomic_DNA.
DR EMBL; DQ056449; AAY78606.1; -; mRNA.
DR PIR; B86228; B86228.
DR RefSeq; NP_172418.2; NM_100818.5.
DR AlphaFoldDB; Q4PT37; -.
DR SMR; Q4PT37; -.
DR STRING; 3702.AT1G09470.1; -.
DR PaxDb; Q4PT37; -.
DR PRIDE; Q4PT37; -.
DR EnsemblPlants; AT1G09470.1; AT1G09470.1; AT1G09470.
DR GeneID; 837470; -.
DR Gramene; AT1G09470.1; AT1G09470.1; AT1G09470.
DR KEGG; ath:AT1G09470; -.
DR Araport; AT1G09470; -.
DR TAIR; locus:2012370; AT1G09470.
DR eggNOG; ENOG502QUNG; Eukaryota.
DR HOGENOM; CLU_077822_0_0_1; -.
DR InParanoid; Q4PT37; -.
DR OMA; FNNQNFW; -.
DR OrthoDB; 1023966at2759; -.
DR PhylomeDB; Q4PT37; -.
DR PRO; PR:Q4PT37; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q4PT37; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005635; C:nuclear envelope; IDA:TAIR.
DR GO; GO:0005637; C:nuclear inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IDA:TAIR.
DR GO; GO:0043621; F:protein self-association; IPI:UniProtKB.
PE 1: Evidence at protein level;
KW Coiled coil; Membrane; Nucleus; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..336
FT /note="Nuclear envelope-associated protein 3"
FT /id="PRO_0000441688"
FT TRANSMEM 313..330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT COILED 14..87
FT /evidence="ECO:0000255"
FT COILED 128..261
FT /evidence="ECO:0000255"
FT MOTIF 240..261
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000305|PubMed:27630107"
SQ SEQUENCE 336 AA; 39001 MW; E21B6B8DD3644484 CRC64;
MPTSVSLRED DPLLKDLSEK KQSFRRNVVS LATELKEART RLAEQERSCS KEAMSRQEAE
TRVKRMEDEM HELAKELNEK VEQIRASDVA TEKFVKELAD IKSQLAATHA TAEASALSAE
SAHSHCRVLS KQLHERTGSL KEHEDQVTRL GEQLENLRKE LRVRESSQKQ LRDELLKVEG
DIMRAVSVVK TKENSEVRNM LNEDTPKNSE RINKLLTAKD DEIARLRDEL KIISAHWRFK
TKELEDQVEN QRRIDQELKK KVLKLEFCLR ETRIQTRKLQ KMGERNDVAI QELKEQLAAK
KQHEADHSSN QNLWDKSGFK IVVSMSMLIL VAFSRR
