ID   NEC1_NICPL              Reviewed;         229 AA.
AC   Q9SPV5;
DT   29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Nectarin-1;
DE            EC=1.15.1.1;
DE   AltName: Full=Superoxide dismutase [Mn];
DE   Flags: Precursor;
GN   Name=NEC1;
OS   Nicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4092;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 129-137.
RX   PubMed=10579488; DOI=10.1023/a:1006363508648;
RA   Carter C., Graham R.A., Thornburg R.W.;
RT   "Nectarin I is a novel, soluble germin-like protein expressed in the nectar
RT   of Nicotiana sp.";
RL   Plant Mol. Biol. 41:207-216(1999).
CC   -!- FUNCTION: May interact with bacterial adhesins thereby protecting the
CC       reproductive tissues from microbial attack. Has no oxalate oxidase
CC       activity (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + 2 superoxide = H2O2 + O2; Xref=Rhea:RHEA:20696,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240,
CC         ChEBI:CHEBI:18421; EC=1.15.1.1;
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 1 Mn(2+) ion per monomer. {ECO:0000250};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Temperature dependence:
CC         Highly thermostable.;
CC   -!- SUBUNIT: Monomer. In the absence of manganese, it forms tetrameric and
CC       pentameric forms which show superoxide dismutase activity (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC       {ECO:0000250}. Note=Secreted in the nectar. {ECO:0000250}.
CC   -!- PTM: Glycosylated. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the germin family. {ECO:0000305}.
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DR   EMBL; AF132671; AAF03355.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9SPV5; -.
DR   SMR; Q9SPV5; -.
DR   PRIDE; Q9SPV5; -.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR   GO; GO:0004784; F:superoxide dismutase activity; IEA:UniProtKB-EC.
DR   Gene3D; 2.60.120.10; -; 1.
DR   InterPro; IPR006045; Cupin_1.
DR   InterPro; IPR001929; Germin.
DR   InterPro; IPR019780; Germin_Mn-BS.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   Pfam; PF00190; Cupin_1; 1.
DR   PRINTS; PR00325; GERMIN.
DR   SMART; SM00835; Cupin_1; 1.
DR   SUPFAM; SSF51182; SSF51182; 1.
DR   PROSITE; PS00725; GERMIN; 1.
PE   1: Evidence at protein level;
KW   Apoplast; Direct protein sequencing; Disulfide bond; Glycoprotein;
KW   Manganese; Metal-binding; Oxidoreductase; Secreted; Signal.
FT   SIGNAL          1..32
FT                   /evidence="ECO:0000250"
FT   CHAIN           33..229
FT                   /note="Nectarin-1"
FT                   /id="PRO_0000010841"
FT   DOMAIN          69..217
FT                   /note="Cupin type-1"
FT                   /evidence="ECO:0000255"
FT   BINDING         117
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         119
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         124
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         163
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        60
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        42..57
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   229 AA;  24765 MW;  DBB8AE087F8256DE CRC64;
     MAAFGIKSKI FQIMEMTILF LFAISIDRYC FAADEDMLQD VCVADLHSKV KVNGFPCKTN
     FTAADFSSFA ISKPGATNNK FGSKVTTANV EQVPGLNTLG VSLARIDYAP GGINPPHTHP
     RASEMVFVME GELDVGFITT ANVLVSKQIT KGEVFVFPRG LVHFQKNNGK IPAAVVSAFN
     SQLPGTQSIP ITLFGASPTV PDDVLAQTFQ INIEDVQQIK SKFAPAKKF