NEC1_PETHY
ID NEC1_PETHY Reviewed; 265 AA.
AC Q9FPN0;
DT 08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 47.
DE RecName: Full=Bidirectional sugar transporter NEC1;
DE AltName: Full=NEC1;
GN Name=NEC1;
OS Petunia hybrida (Petunia).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Petunioideae; Petunia.
OX NCBI_TaxID=4102;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=11135107; DOI=10.1046/j.1365-313x.2000.00926.x;
RA Ge Y.-X., Angenent G.C., Wittich P.E., Peters J., Franken J., Busscher M.,
RA Zhang L.M., Dahlhaus E., Kater M.M., Wullems G.J., Creemers-Molenaar T.;
RT "NEC1, a novel gene, highly expressed in nectary tissue of Petunia
RT hybrida.";
RL Plant J. 24:725-734(2000).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. W115, and cv. W138;
RX PubMed=11405624; DOI=10.1007/s004380100449;
RA Ge Y.-X., Angenent G.C., Dahlhaus E., Franken J., Peters J., Wullems G.J.,
RA Creemers-Molenaar J.;
RT "Partial silencing of the NEC1 gene results in early opening of anthers in
RT Petunia hybrida.";
RL Mol. Genet. Genomics 265:414-423(2001).
CC -!- FUNCTION: Mediates both low-affinity uptake and efflux of sugar across
CC the plasma membrane. Promotes the formation of phloem bundles in mid-
CC veins. Probably involved in the development of stomium cells that
CC control anther opening time. Required for pollen viability
CC (PubMed:11135107, PubMed:11405624). {ECO:0000250|UniProtKB:Q8L9J7,
CC ECO:0000269|PubMed:11135107, ECO:0000269|PubMed:11405624}.
CC -!- SUBUNIT: Forms homooligomers and/or heterooligomers.
CC {ECO:0000250|UniProtKB:Q8L9J7}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q8L9J7};
CC Multi-pass membrane protein {ECO:0000250|UniProtKB:Q8L9J7}.
CC -!- TISSUE SPECIFICITY: Highly expressed in nectary tissue and weakly in
CC the stamen, especially in stomium cells and in the upper part of the
CC filaments. {ECO:0000269|PubMed:11135107}.
CC -!- DEVELOPMENTAL STAGE: Detected in the outer nectary parenchyma cells,
CC the upper part of the filament and the anther stomium. Expressed in
CC spots pattern on the surface of very young nectaries that do not
CC secrete nectar and do accumulate starch. Highest levels in open flowers
CC in which active secretion of nectar and starch hydrolysis have taken
CC place. {ECO:0000269|PubMed:11135107}.
CC -!- DISRUPTION PHENOTYPE: 'Early open anther' phenotype; anther opening in
CC young flower buds that contain immature pollen, resulting in poor
CC pollen quality and impaired pollen release.
CC {ECO:0000269|PubMed:11405624}.
CC -!- SIMILARITY: Belongs to the SWEET sugar transporter family.
CC {ECO:0000305}.
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DR EMBL; AF313914; AAG34696.1; -; mRNA.
DR AlphaFoldDB; Q9FPN0; -.
DR SMR; Q9FPN0; -.
DR TCDB; 2.A.123.1.7; the sweet, pq-loop, saliva, mtn3 (sweet) family.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:UniProtKB.
DR GO; GO:0051119; F:sugar transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:0009901; P:anther dehiscence; IMP:UniProtKB.
DR GO; GO:0009555; P:pollen development; IMP:UniProtKB.
DR InterPro; IPR004316; SWEET_sugar_transpr.
DR Pfam; PF03083; MtN3_slv; 2.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Repeat; Sugar transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..265
FT /note="Bidirectional sugar transporter NEC1"
FT /id="PRO_0000404151"
FT TOPO_DOM 1..8
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 9..29
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 30..44
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 66..71
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 72..92
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 93..103
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 125..130
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..151
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 152..164
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..185
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 186..190
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 212..265
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 11..97
FT /note="MtN3/slv 1"
FT DOMAIN 132..216
FT /note="MtN3/slv 2"
SQ SEQUENCE 265 AA; 30256 MW; E6C6906B72A40C53 CRC64;
MAQLRADDLS FIFGLLGNIV SFMVFLAPVP TFYKIYKRKS SEGYQAIPYM VALFSAGLLL
YYAYLRKNAY LIVSINGFGC AIELTYISLF LFYAPRKSKI FTGWLMLLEL GALGMVMPIT
YLLAEGSHRV MIVGWICAAI NVAVFAAPLS IMRQVIKTKS VEFMPFTLSL FLTLCATMWF
FYGFFKKDFY IAFPNILGFL FGIVQMLLYF VYKDSKRIDD EKSDPVREAT KSKEGVEIII
NIEDDNSDNA LQSMEKDFSR LRTSK
