ID   NEC2_MAGO7              Reviewed;         265 AA.
AC   G4NEA9;
DT   13-FEB-2019, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 1.
DT   03-AUG-2022, entry version 34.
DE   RecName: Full=O-methyltransferase NEC2 {ECO:0000303|PubMed:30443971};
DE            EC=2.1.1.- {ECO:0000269|PubMed:30443971};
DE   AltName: Full=Nectriapyrone biosynthesis cluster protein 2 {ECO:0000303|PubMed:30443971};
GN   Name=NEC2 {ECO:0000303|PubMed:30443971}; ORFNames=MGG_14657;
OS   Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast
OS   fungus) (Pyricularia oryzae).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Magnaporthales; Pyriculariaceae; Pyricularia.
OX   NCBI_TaxID=242507;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=70-15 / ATCC MYA-4617 / FGSC 8958;
RX   PubMed=15846337; DOI=10.1038/nature03449;
RA   Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K.,
RA   Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D.,
RA   Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S.,
RA   Soanes D.M., Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M.,
RA   Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E.,
RA   Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W.;
RT   "The genome sequence of the rice blast fungus Magnaporthe grisea.";
RL   Nature 434:980-986(2005).
RN   [2]
RP   IDENTIFICATION, INDUCTION, DISRUPTION PHENOTYPE, FUNCTION, AND CATALYTIC
RP   ACTIVITY.
RX   PubMed=30443971; DOI=10.1002/cbic.201800620;
RA   Motoyama T., Nogawa T., Hayashi T., Hirota H., Osada H.;
RT   "Induction of nectriapyrone biosynthesis in the rice blast fungus
RT   Pyricularia oryzae by disturbance of the two-component signal transduction
RT   system.";
RL   ChemBioChem 20:693-700(2019).
CC   -!- FUNCTION: O-methyltransferase; part of the gene cluster that mediates
CC       the biosynthesis of nectriapyrone and its analogs phomopyrone A,
CC       acropyrone and zaepyrone (PubMed:30443971). The nectriapyrone
CC       biosynthetic gene cluster consists of two genes, the highly reducing
CC       polyketide synthase NEC1 that produces a demethylated analog of
CC       nectriapyrone from one unit of acetyl-CoA and one unit of malonyl-CoA;
CC       and the O-methyltransferase NEC2 that further methylates the NEC1
CC       product to yield nectriapyrone (PubMed:30443971). Nectriapyrone is
CC       further hydrolyzed to nectriapyrone D, also known as gulypyrone B, by
CC       an unidentified hydrolase localized outside the nectriapyrone cluster
CC       (PubMed:30443971). {ECO:0000269|PubMed:30443971}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=desmethylnectriapyrone + S-adenosyl-L-methionine = H(+) +
CC         nectriapyrone + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:58748,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:142639, ChEBI:CHEBI:142640;
CC         Evidence={ECO:0000269|PubMed:30443971};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:58749;
CC         Evidence={ECO:0000269|PubMed:30443971};
CC   -!- DISRUPTION PHENOTYPE: Completely abolishes the production of
CC       nectriapyrones but accumulates a demethylated analog of nectriapyrone.
CC       {ECO:0000269|PubMed:30443971}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC       {ECO:0000255}.
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DR   EMBL; CM001235; EHA48592.1; -; Genomic_DNA.
DR   RefSeq; XP_003718176.1; XM_003718128.1.
DR   AlphaFoldDB; G4NEA9; -.
DR   EnsemblFungi; MGG_14657T0; MGG_14657T0; MGG_14657.
DR   GeneID; 5050752; -.
DR   KEGG; mgr:MGG_14657; -.
DR   VEuPathDB; FungiDB:MGG_14657; -.
DR   eggNOG; ENOG502SJSY; Eukaryota.
DR   HOGENOM; CLU_046029_0_0_1; -.
DR   InParanoid; G4NEA9; -.
DR   OMA; LQHDIFT; -.
DR   OrthoDB; 1170760at2759; -.
DR   Proteomes; UP000009058; Chromosome 5.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   1: Evidence at protein level;
KW   Methyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..265
FT                   /note="O-methyltransferase NEC2"
FT                   /id="PRO_0000446266"
SQ   SEQUENCE   265 AA;  29680 MW;  516221872DE9D2AB CRC64;
     MASSRDRAYV TVYSPTLLTW VYDYYVLGFN LRYIWGCPTD AVLLPFFAEN FSRRHLDVGV
     ATGYLPARVL ASPWRRAAAH RQHLTLLDIN ANSLRASDAR VRAAAPGIET TCVEADVVAD
     LPPVLASVVE EERARVEKAG GEAGSSSRSC LYDSISMFNL FHCVPGGPAK LRAISTYKAL
     LADHGVLSGC TVLGERHATG WFSRWYLRLY NRKGIFNNIN DTREEFEEVL NKEFEEVDTW
     MFGMVLLFRA SKPRREGSGY VDLLA