ID   NEC2_NESMA              Reviewed;         311 AA.
AC   P0DO51;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=Ferritin-like catalase Nec2 {ECO:0000303|PubMed:35074876};
DE            EC=1.11.1.6 {ECO:0000269|PubMed:35074876};
DE   AltName: Full=Nectar protein 2 {ECO:0000303|PubMed:35074876};
DE            Short=NmNec2 {ECO:0000303|PubMed:35074876};
DE   Flags: Precursor;
GN   Name=NEC2 {ECO:0000303|PubMed:35074876};
OS   Nesocodon mauritianus (Blue Mauritius bellflower) (Wahlenbergia
OS   mauritiana).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Asterales; Campanulaceae; Nesocodon.
OX   NCBI_TaxID=519296;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE
RP   SPECIFICITY, AND SUBUNIT.
RX   PubMed=35074876; DOI=10.1073/pnas.2114420119;
RA   Roy R., Moreno N., Brockman S.A., Kostanecki A., Zambre A., Holl C.,
RA   Solhaug E.M., Minami A., Snell-Rood E.C., Hampton M., Bee M.A., Chiari Y.,
RA   Hegeman A.D., Carter C.J.;
RT   "Convergent evolution of a blood-red nectar pigment in vertebrate-
RT   pollinated flowers.";
RL   Proc. Natl. Acad. Sci. U.S.A. 119:0-0(2022).
CC   -!- FUNCTION: Involved in the production of blood-red nectar containing the
CC       alkaloid nesocodin and that serves as a visual attractant for
CC       pollinator visitation, including vertebrates such as Phelsuma geckos
CC       (PubMed:35074876). The nectar is initially acidic and pale yellow, but
CC       slowly becomes alkaline before turning into red within 24 hours
CC       (PubMed:35074876). Together with NEC1 and NEC3, facilitates the
CC       condensation of sinapaldehyde ((E)-3,5-dimethoxy-4-
CC       hydroxycinnamaldehyde) and proline to form nesocodin, a pigment with a
CC       stable imine bond (PubMed:35074876). Protects nesocodin from
CC       degradation by hydrogen peroxide H(2)O(2) by catalyzing the degradation
CC       of H(2)O(2) into water H(2)O and dioxygene O(2) (PubMed:35074876).
CC       {ECO:0000269|PubMed:35074876}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H2O2 = 2 H2O + O2; Xref=Rhea:RHEA:20309, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:16240; EC=1.11.1.6;
CC         Evidence={ECO:0000269|PubMed:35074876};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:20310;
CC         Evidence={ECO:0000269|PubMed:35074876};
CC   -!- SUBUNIT: Forms homomultimers. {ECO:0000269|PubMed:35074876}.
CC   -!- TISSUE SPECIFICITY: Observed in all flowers organs; mainly expressed in
CC       nectaries and, to a lower extent, in petals and ovules, as well as in
CC       stigmas and calyx at low levels. {ECO:0000269|PubMed:35074876}.
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DR   EMBL; OK664973.1; UIE54577.1; -; mRNA.
PE   1: Evidence at protein level;
KW   Glycoprotein; Oxidoreductase; Signal.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..311
FT                   /note="Ferritin-like catalase Nec2"
FT                   /id="PRO_0000456306"
FT   CARBOHYD        128
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        257
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        289
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   311 AA;  34250 MW;  C9E315E5EA60EE71 CRC64;
     MAFLSNMAMF ITMLMFSSMM HPCFSQPSCA PMDTMDTNGV DEADIDMMQF PINLEFLEAE
     FFLWGALGHG LDVVAPQLAM GGPPPYGAQK ANLDPLTQNI ITEFAYQEVG HLRALERTVG
     GFPRPLLNLS ASNFANLIEA AFGYHLVPPF NPYRDGLSYM LASYAVPYMG LVGYVGTNPN
     LKGHQTKRLL AGLLGVESGQ DAVIRMYLYE RQGHVVAPYR HTVAEFTARI SELRNRLAMC
     GVKDEGVIVP RQLGAENQTM SNVLSANYDS ISYRRTPGEI LRVVYSTGNE SVPGGFYPNG
     GNGRIARRFL V