NEC2_SHV21
ID NEC2_SHV21 Reviewed; 235 AA.
AC Q01045;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 1.
DT 02-DEC-2020, entry version 61.
DE RecName: Full=Nuclear egress protein 2 {ECO:0000255|HAMAP-Rule:MF_04024};
GN Name=NEC2 {ECO:0000255|HAMAP-Rule:MF_04024}; OrderedLocusNames=67, ECLF5;
OS Saimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri).
OC Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC Herpesvirales; Herpesviridae; Gammaherpesvirinae; Rhadinovirus.
OX NCBI_TaxID=10383;
OH NCBI_TaxID=9521; Saimiri sciureus (Common squirrel monkey).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=1321287; DOI=10.1128/jvi.66.8.5047-5058.1992;
RA Albrecht J.-C., Nicholas J., Biller D., Cameron K.R., Biesinger B.,
RA Newman C., Wittmann S., Craxton M.A., Coleman H., Fleckenstein B.,
RA Honess R.W.;
RT "Primary structure of the herpesvirus saimiri genome.";
RL J. Virol. 66:5047-5058(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1314457; DOI=10.1016/0042-6822(92)90759-i;
RA Nicholas J., Cameron K.R., Coleman H., Newman C., Honess R.W.;
RT "Analysis of nucleotide sequence of the rightmost 43 kbp of herpesvirus
RT saimiri (HVS) L-DNA: general conservation of genetic organization between
RT HVS and Epstein-Barr virus.";
RL Virology 188:296-310(1992).
CC -!- FUNCTION: Plays an essential role in virion nuclear egress, the first
CC step of virion release from infected cell. Within the host nucleus,
CC NEC1 interacts with the newly formed capsid through the vertexes and
CC directs it to the inner nuclear membrane by associating with NEC2.
CC Induces the budding of the capsid at the inner nuclear membrane as well
CC as its envelopment into the perinuclear space. There, the NEC1/NEC2
CC complex promotes the fusion of the enveloped capsid with the outer
CC nuclear membrane and the subsequent release of the viral capsid into
CC the cytoplasm where it will reach the secondary budding sites in the
CC host Golgi or trans-Golgi network. {ECO:0000255|HAMAP-Rule:MF_04024}.
CC -!- SUBUNIT: Forms a heterohexameric complex with NEC1. {ECO:0000255|HAMAP-
CC Rule:MF_04024}.
CC -!- SUBCELLULAR LOCATION: Host nucleus inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_04024}; Single-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_04024}. Note=Localizes also at the transient membrane of
CC perinuclear virions. {ECO:0000255|HAMAP-Rule:MF_04024}.
CC -!- PTM: Phosphorylated. {ECO:0000255|HAMAP-Rule:MF_04024}.
CC -!- SIMILARITY: Belongs to the herpesviridae NEC2 protein family.
CC {ECO:0000255|HAMAP-Rule:MF_04024}.
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DR EMBL; X64346; CAA45690.1; -; Genomic_DNA.
DR EMBL; M86409; AAA46143.1; -; Genomic_DNA.
DR RefSeq; NP_040269.1; NC_001350.1.
DR SMR; Q01045; -.
DR PRIDE; Q01045; -.
DR GeneID; 1682481; -.
DR KEGG; vg:1682481; -.
DR Proteomes; UP000000587; Genome.
DR GO; GO:0044201; C:host cell nuclear inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR HAMAP; MF_04024; HSV_NEC2; 1.
DR InterPro; IPR007626; Herpesvirus_viron_egress-type.
DR Pfam; PF04541; Herpes_U34; 1.
PE 3: Inferred from homology;
KW Host membrane; Host nucleus; Late protein; Membrane; Phosphoprotein;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..235
FT /note="Nuclear egress protein 2"
FT /id="PRO_0000116032"
FT TOPO_DOM 1..214
FT /note="Perinuclear space"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04024"
FT TRANSMEM 215..232
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04024"
FT TOPO_DOM 233..235
FT /note="Nuclear"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04024"
SQ SEQUENCE 235 AA; 26886 MW; 4463078F2376DEC6 CRC64;
MNSTRLVYEL CDIVNLYLCQ PGVQIDVDRC ASGPHVFTKG GTEAICTVKL SHGLVYNIEF
VYKFWAHKLE SVKYPFSPCF IISNNGLATT LKCFLSRPRN VNHFGHVLNI DSDVYLTKNT
SVILSQDDFV KFKTNLVFSK DLDVFHSMVV FRTYLIEHRQ ALQFLVVKPR SSKRVNSILS
SVAKTASQNF ILDPPRRSEE TRVCIKPWTL SKKNIWTIIL SLVAVVAIIL KWREL