NECA1_HUMAN
ID NECA1_HUMAN Reviewed; 351 AA.
AC Q8N987; Q6NUS7; Q96AZ7; Q9HBW8;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=N-terminal EF-hand calcium-binding protein 1;
DE Short=EF-hand calcium-binding protein 1;
DE AltName: Full=Neuronal calcium-binding protein 1;
GN Name=NECAB1; Synonyms=EFCBP1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Mottillo E.P., Sullivan J.M., Patel V., Lepeak W., Elferink L.A.,
RA Hubberstey A.V.;
RT "Necab1: a novel human neuronal protein that interacts with cyclase
RT associated proteins.";
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Guo J.H., Yu L.;
RT "Molecular cloning and characterization of human NECAB1 gene.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain, and Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 97-351 (ISOFORM 1), INTERACTION WITH STX1 AND
RP CPNE6, AND TISSUE SPECIFICITY.
RC TISSUE=Brain;
RX PubMed=12044471; DOI=10.1016/s0306-4522(02)00063-5;
RA Sugita S., Ho A., Suedhof T.C.;
RT "NECABs: a family of neuronal Ca(2+)-binding proteins with an unusual
RT domain structure and a restricted expression pattern.";
RL Neuroscience 112:51-63(2002).
CC -!- SUBUNIT: Interacts with STX1 (PubMed:12044471). May interact with CPNE6
CC (PubMed:12044471). {ECO:0000269|PubMed:12044471}.
CC -!- INTERACTION:
CC Q8N987; Q9BXS5: AP1M1; NbExp=3; IntAct=EBI-11956853, EBI-541426;
CC Q8N987; O00244: ATOX1; NbExp=3; IntAct=EBI-11956853, EBI-10179267;
CC Q8N987; Q7RTU4: BHLHA9; NbExp=3; IntAct=EBI-11956853, EBI-17508719;
CC Q8N987; P55201-2: BRPF1; NbExp=3; IntAct=EBI-11956853, EBI-12065306;
CC Q8N987; Q16543: CDC37; NbExp=3; IntAct=EBI-11956853, EBI-295634;
CC Q8N987; Q96A23-2: CPNE4; NbExp=6; IntAct=EBI-11956853, EBI-12302039;
CC Q8N987; Q9UBL6-2: CPNE7; NbExp=7; IntAct=EBI-11956853, EBI-12012272;
CC Q8N987; Q86YQ8: CPNE8; NbExp=3; IntAct=EBI-11956853, EBI-1642325;
CC Q8N987; Q9NRI5-2: DISC1; NbExp=3; IntAct=EBI-11956853, EBI-11988027;
CC Q8N987; Q8TBB1: LNX1; NbExp=3; IntAct=EBI-11956853, EBI-739832;
CC Q8N987; Q9UBU8-2: MORF4L1; NbExp=3; IntAct=EBI-11956853, EBI-10288852;
CC Q8N987; Q8N987: NECAB1; NbExp=5; IntAct=EBI-11956853, EBI-11956853;
CC Q8N987; Q7Z6G3-2: NECAB2; NbExp=3; IntAct=EBI-11956853, EBI-10172876;
CC Q8N987; Q3KNR5: PAX4; NbExp=3; IntAct=EBI-11956853, EBI-10240813;
CC Q8N987; Q9Y5W9: SNX11; NbExp=3; IntAct=EBI-11956853, EBI-10329449;
CC Q8N987; Q15560: TCEA2; NbExp=3; IntAct=EBI-11956853, EBI-710310;
CC Q8N987; Q8N8B7-2: TCEANC; NbExp=3; IntAct=EBI-11956853, EBI-11955057;
CC Q8N987; Q6ZN96; NbExp=3; IntAct=EBI-11956853, EBI-10255097;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8N987-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8N987-2; Sequence=VSP_024214;
CC -!- TISSUE SPECIFICITY: Expressed in brain (at protein level).
CC {ECO:0000269|PubMed:12044471}.
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DR EMBL; AY224210; AAO34126.1; -; mRNA.
DR EMBL; AF414126; AAP33146.1; -; mRNA.
DR EMBL; AK095533; BAC04568.1; -; mRNA.
DR EMBL; BC016340; AAH16340.1; -; mRNA.
DR EMBL; BC068448; AAH68448.1; -; mRNA.
DR EMBL; AF193756; AAG28412.1; -; mRNA.
DR CCDS; CCDS47889.1; -. [Q8N987-1]
DR RefSeq; NP_071746.1; NM_022351.4. [Q8N987-1]
DR AlphaFoldDB; Q8N987; -.
DR SMR; Q8N987; -.
DR BioGRID; 122093; 32.
DR IntAct; Q8N987; 29.
DR STRING; 9606.ENSP00000387380; -.
DR iPTMnet; Q8N987; -.
DR PhosphoSitePlus; Q8N987; -.
DR BioMuta; NECAB1; -.
DR DMDM; 74760025; -.
DR MassIVE; Q8N987; -.
DR PaxDb; Q8N987; -.
DR PeptideAtlas; Q8N987; -.
DR PRIDE; Q8N987; -.
DR ProteomicsDB; 72503; -. [Q8N987-1]
DR ProteomicsDB; 72504; -. [Q8N987-2]
DR Antibodypedia; 6629; 209 antibodies from 21 providers.
DR DNASU; 64168; -.
DR Ensembl; ENST00000417640.7; ENSP00000387380.2; ENSG00000123119.12. [Q8N987-1]
DR Ensembl; ENST00000521366.1; ENSP00000428632.1; ENSG00000123119.12. [Q8N987-2]
DR Ensembl; ENST00000522820.5; ENSP00000428953.1; ENSG00000123119.12. [Q8N987-2]
DR GeneID; 64168; -.
DR KEGG; hsa:64168; -.
DR MANE-Select; ENST00000417640.7; ENSP00000387380.2; NM_022351.5; NP_071746.1.
DR UCSC; uc003yer.4; human. [Q8N987-1]
DR CTD; 64168; -.
DR DisGeNET; 64168; -.
DR GeneCards; NECAB1; -.
DR HGNC; HGNC:20983; NECAB1.
DR HPA; ENSG00000123119; Group enriched (brain, choroid plexus).
DR neXtProt; NX_Q8N987; -.
DR OpenTargets; ENSG00000123119; -.
DR PharmGKB; PA162397411; -.
DR VEuPathDB; HostDB:ENSG00000123119; -.
DR eggNOG; ENOG502QWRY; Eukaryota.
DR GeneTree; ENSGT00950000183131; -.
DR HOGENOM; CLU_041553_0_0_1; -.
DR InParanoid; Q8N987; -.
DR OMA; EVLLIQW; -.
DR OrthoDB; 924307at2759; -.
DR PhylomeDB; Q8N987; -.
DR TreeFam; TF331029; -.
DR PathwayCommons; Q8N987; -.
DR SignaLink; Q8N987; -.
DR BioGRID-ORCS; 64168; 11 hits in 1078 CRISPR screens.
DR ChiTaRS; NECAB1; human.
DR GenomeRNAi; 64168; -.
DR Pharos; Q8N987; Tbio.
DR PRO; PR:Q8N987; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; Q8N987; protein.
DR Bgee; ENSG00000123119; Expressed in Brodmann (1909) area 9 and 118 other tissues.
DR Genevisible; Q8N987; HS.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0001835; P:blastocyst hatching; IEA:Ensembl.
DR GO; GO:0042984; P:regulation of amyloid precursor protein biosynthetic process; IBA:GO_Central.
DR InterPro; IPR007138; ABM_dom.
DR InterPro; IPR011008; Dimeric_a/b-barrel.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR039862; NECAB1/2/3.
DR PANTHER; PTHR12178; PTHR12178; 1.
DR Pfam; PF03992; ABM; 1.
DR Pfam; PF13833; EF-hand_8; 1.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; SSF47473; 1.
DR SUPFAM; SSF54909; SSF54909; 1.
DR PROSITE; PS51725; ABM; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Calcium; Coiled coil; Cytoplasm; Metal-binding;
KW Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..351
FT /note="N-terminal EF-hand calcium-binding protein 1"
FT /id="PRO_0000282609"
FT DOMAIN 26..61
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 60..95
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 252..340
FT /note="ABM"
FT REGION 180..203
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 135..163
FT /evidence="ECO:0000255"
FT COILED 209..275
FT /evidence="ECO:0000255"
FT COMPBIAS 189..203
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 39
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 41
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 43
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 45
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 50
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT MOD_RES 4
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9ESB5"
FT MOD_RES 192
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8BG18"
FT MOD_RES 197
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8BG18"
FT VAR_SEQ 1..251
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_024214"
FT CONFLICT 105
FT /note="D -> G (in Ref. 4; AAH68448)"
FT /evidence="ECO:0000305"
FT CONFLICT 261
FT /note="I -> M (in Ref. 4; AAH16340)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 351 AA; 40571 MW; 74D5E5895B4FECFA CRC64;
MEDSQETSPS SNNSSEELSS ALHLSKGMSI FLDILRRADK NDDGKLSFEE FKAYFADGVL
SGEELHELFH TIDTHNTNNL DTEELCEYFS QHLGEYENVL AALEDLNLSI LKAMGKTKKD
YQEASNLEQF VTRFLLKETL NQLQSLQNSL ECAMETTEEQ TRQERQGPAK PEVLSIQWPG
KRSSRRVQRH NSFSPNSPQF NVSGPGLLEE DNQWMTQINR LQKLIDRLEK KDLKLEPPEE
EIIEGNTKSH IMLVQRQMSV IEEDLEEFQL ALKHYVESAS SQSGCLRISI QKLSNESRYM
IYEFWENSSV WNSHLQTNYS KTFQRSNVDF LETPELTSTM LVPASWWILN N
