NECD_MOUSE
ID NECD_MOUSE Reviewed; 325 AA.
AC P25233; B9EJJ5; Q542W7; Q61951;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1992, sequence version 1.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Necdin;
GN Name=Ndn;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Embryonic carcinoma;
RX PubMed=2069569; DOI=10.1016/0006-291x(91)91812-q;
RA Maruyama K., Usami M., Aizawa T., Yoshikawa K.;
RT "A novel brain-specific mRNA encoding nuclear protein (necdin) expressed in
RT neurally differentiated embryonal carcinoma cells.";
RL Biochem. Biophys. Res. Commun. 178:291-296(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT ALA-17.
RC STRAIN=129/Sv;
RX PubMed=8557705; DOI=10.1074/jbc.271.2.918;
RA Uetsuki T., Takagi K., Sugiura H., Yoshikawa K.;
RT "Structure and expression of the mouse necdin gene. Identification of a
RT postmitotic neuron-restrictive core promoter.";
RL J. Biol. Chem. 271:918-924(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J;
RC TISSUE=Cerebellum, Muellerian duct, and Sympathetic ganglion;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP INTERACTION WITH VIRAL TRANSFORMING PROTEINS AND EF21.
RX PubMed=9422723; DOI=10.1074/jbc.273.2.720;
RA Taniura H., Taniguchi N., Hara M., Yoshikawa K.;
RT "Necdin, a postmitotic neuron-specific growth suppressor, interacts with
RT viral transforming proteins and cellular transcription factor E2F1.";
RL J. Biol. Chem. 273:720-728(1998).
RN [6]
RP INTERACTION WITH TP53.
RX PubMed=10347180; DOI=10.1074/jbc.274.23.16242;
RA Taniura H., Matsumoto K., Yoshikawa K.;
RT "Physical and functional interactions of neuronal growth suppressor necdin
RT with p53.";
RL J. Biol. Chem. 274:16242-16248(1999).
RN [7]
RP SUBCELLULAR LOCATION.
RX PubMed=10965153; DOI=10.1159/000017455;
RA Niinobe M., Koyama K., Yoshikawa K.;
RT "Cellular and subcellular localization of necdin in fetal and adult mouse
RT brain.";
RL Dev. Neurosci. 22:310-319(2000).
CC -!- FUNCTION: Growth suppressor that facilitates the entry of the cell into
CC cell cycle arrest. Functionally similar to the retinoblastoma protein
CC it binds to and represses the activity of cell-cycle-promoting proteins
CC such as SV40 large T antigen, adenovirus E1A, and the transcription
CC factor E2F. Necdin also interacts with p53 and works in an additive
CC manner to inhibit cell growth. Also functions as a transcription factor
CC and directly binds to specific guanosine-rich DNA sequences.
CC -!- SUBUNIT: Binds to the transactivation domains of E2F1 and p53. Binds
CC also SV40 large T antigen and adenovirus E1A. Interacts with
CC nucleobindin 1 and 2. {ECO:0000269|PubMed:10347180,
CC ECO:0000269|PubMed:9422723}.
CC -!- INTERACTION:
CC P25233; Q61501: E2f1; NbExp=6; IntAct=EBI-1801080, EBI-1025536;
CC P25233; Q9QYH6: Maged1; NbExp=7; IntAct=EBI-1801080, EBI-1801274;
CC P25233; P13297: Msx1; NbExp=2; IntAct=EBI-1801080, EBI-903969;
CC P25233; Q03358: Msx2; NbExp=3; IntAct=EBI-1801080, EBI-1801354;
CC P25233; Q9Y6B2: EID1; Xeno; NbExp=5; IntAct=EBI-1801080, EBI-1049975;
CC P25233; P08138: NGFR; Xeno; NbExp=3; IntAct=EBI-1801080, EBI-1387782;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:10965153}. Nucleus,
CC nucleoplasm {ECO:0000269|PubMed:10965153}. Nucleus matrix
CC {ECO:0000269|PubMed:10965153}. Note=Mainly cytoplasmic. Translocates to
CC the nucleus where it is found associated with the nuclear matrix.
CC -!- TISSUE SPECIFICITY: Brain specific. Not detected in other tissues.
CC Expressed in postmitotic neurons. In adult brain the highest expression
CC is in hypothalamus. Highly expressed in thalamus and midbrain.
CC Relatively low levels are in cerebral cortex, hippocampus, striatum,
CC olfactory bulb, cerebellum, pons and spinal cord. Also detected in
CC neurally differentiated embryonal carcinoma cells.
CC -!- DEVELOPMENTAL STAGE: Expression levels were high during embryonic and
CC neonatal periods (14 dpc to P7) and decreased thereafter.
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DR EMBL; M80840; AAA39805.1; -; mRNA.
DR EMBL; D76440; BAA11183.1; -; Genomic_DNA.
DR EMBL; AK005078; BAB23802.1; -; mRNA.
DR EMBL; AK075729; BAC35914.1; -; mRNA.
DR EMBL; AK075735; BAC35917.1; -; mRNA.
DR EMBL; AK076025; BAC36130.1; -; mRNA.
DR EMBL; AK135444; BAE22535.1; -; mRNA.
DR EMBL; AK149086; BAE28734.1; -; mRNA.
DR EMBL; BC147249; AAI47250.1; -; mRNA.
DR EMBL; BC147250; AAI47251.1; -; mRNA.
DR CCDS; CCDS21326.1; -.
DR PIR; JN0148; JN0148.
DR RefSeq; NP_035012.2; NM_010882.3.
DR AlphaFoldDB; P25233; -.
DR SMR; P25233; -.
DR BioGRID; 201712; 84.
DR IntAct; P25233; 9.
DR MINT; P25233; -.
DR STRING; 10090.ENSMUSP00000045369; -.
DR MoonDB; P25233; Predicted.
DR iPTMnet; P25233; -.
DR PhosphoSitePlus; P25233; -.
DR MaxQB; P25233; -.
DR PaxDb; P25233; -.
DR PRIDE; P25233; -.
DR ProteomicsDB; 287472; -.
DR Antibodypedia; 22276; 368 antibodies from 26 providers.
DR DNASU; 17984; -.
DR Ensembl; ENSMUST00000038775; ENSMUSP00000045369; ENSMUSG00000033585.
DR GeneID; 17984; -.
DR KEGG; mmu:17984; -.
DR UCSC; uc009hff.1; mouse.
DR CTD; 4692; -.
DR MGI; MGI:97290; Ndn.
DR VEuPathDB; HostDB:ENSMUSG00000033585; -.
DR eggNOG; KOG4562; Eukaryota.
DR GeneTree; ENSGT00940000163084; -.
DR HOGENOM; CLU_039582_1_1_1; -.
DR InParanoid; P25233; -.
DR OMA; EQSKDVC; -.
DR OrthoDB; 1195799at2759; -.
DR PhylomeDB; P25233; -.
DR TreeFam; TF328505; -.
DR BioGRID-ORCS; 17984; 0 hits in 72 CRISPR screens.
DR ChiTaRS; Ndn; mouse.
DR PRO; PR:P25233; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; P25233; protein.
DR Bgee; ENSMUSG00000033585; Expressed in dorsomedial nucleus of hypothalamus and 283 other tissues.
DR Genevisible; P25233; MM.
DR GO; GO:0042995; C:cell projection; IDA:MGI.
DR GO; GO:0005813; C:centrosome; IDA:MGI.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0005829; C:cytosol; IDA:MGI.
DR GO; GO:0016363; C:nuclear matrix; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0032991; C:protein-containing complex; IDA:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:MGI.
DR GO; GO:0043015; F:gamma-tubulin binding; IDA:MGI.
DR GO; GO:1990841; F:promoter-specific chromatin binding; IDA:MGI.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:MGI.
DR GO; GO:0048675; P:axon extension; IMP:MGI.
DR GO; GO:0007413; P:axonal fasciculation; IMP:MGI.
DR GO; GO:0007409; P:axonogenesis; IMP:MGI.
DR GO; GO:0007417; P:central nervous system development; IMP:MGI.
DR GO; GO:0071514; P:genomic imprinting; IMP:MGI.
DR GO; GO:0008347; P:glial cell migration; IMP:MGI.
DR GO; GO:0048871; P:multicellular organismal homeostasis; IMP:MGI.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IEA:InterPro.
DR GO; GO:0043433; P:negative regulation of DNA-binding transcription factor activity; IDA:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:MGI.
DR GO; GO:0048666; P:neuron development; IMP:MGI.
DR GO; GO:0030182; P:neuron differentiation; IDA:MGI.
DR GO; GO:0001764; P:neuron migration; IMP:MGI.
DR GO; GO:0048011; P:neurotrophin TRK receptor signaling pathway; IMP:MGI.
DR GO; GO:0090312; P:positive regulation of protein deacetylation; IDA:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0009791; P:post-embryonic development; IMP:MGI.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IGI:MGI.
DR GO; GO:0007585; P:respiratory gaseous exchange by respiratory system; IMP:MGI.
DR GO; GO:0003016; P:respiratory system process; IMP:MGI.
DR GO; GO:0019233; P:sensory perception of pain; IMP:MGI.
DR Gene3D; 1.10.10.1200; -; 1.
DR Gene3D; 1.10.10.1210; -; 1.
DR InterPro; IPR037445; MAGE.
DR InterPro; IPR041898; MAGE_WH1.
DR InterPro; IPR041899; MAGE_WH2.
DR InterPro; IPR002190; MHD_dom.
DR InterPro; IPR030086; Necdin.
DR PANTHER; PTHR11736; PTHR11736; 1.
DR PANTHER; PTHR11736:SF10; PTHR11736:SF10; 1.
DR Pfam; PF01454; MAGE; 1.
DR SMART; SM01373; MAGE; 1.
DR PROSITE; PS50838; MAGE; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Growth regulation; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..325
FT /note="Necdin"
FT /id="PRO_0000156741"
FT DOMAIN 102..301
FT /note="MAGE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00127"
FT REGION 1..69
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 77..96
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 17
FT /note="V -> A"
FT /evidence="ECO:0000269|PubMed:8557705"
SQ SEQUENCE 325 AA; 36832 MW; 777385B0B75E443F CRC64;
MSEQSKDLSD PNFAAEVPDC EMQDSDAVPV GIPPPASLAA NLAGPPCAPE GPMAAQQASP
PPEERIEDVD PKILQQAAEE GRAHQPQSPA RPIPAPPAPA QLVQKAHELM WYVLVKDQKR
MVLWFPDMVK EVMGSYKKWC RSILRRTSVI LARVFGLHLR LTNLHTMEFA LVKALSPEEL
DRVALNNRMP MTGLLLMILS LIYVKGRGAR EGAVWNVLRI LGLRPWKKHS TFGDVRKIIT
EEFVQQNYLK YQRVPHIEPP EYEFFWGSRA NREITKMQIM EFLARVFKKD PQAWPSRYRE
ALEQARALRE ANLAAQAPRS SVSED
