ID   NECP2_BOVIN             Reviewed;         266 AA.
AC   Q5E9Q4;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Adaptin ear-binding coat-associated protein 2;
DE   AltName: Full=NECAP endocytosis-associated protein 2;
DE            Short=NECAP-2;
GN   Name=NECAP2;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences.";
RL   BMC Genomics 6:166-166(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in endocytosis. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with AP1G1 and AP2A1 components of the adapter
CC       protein complexes AP-1 and AP-2. Interacts with the GAE domain proteins
CC       GGA1, GGA2 and GGA3 (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, clathrin-coated vesicle
CC       membrane {ECO:0000250}. Cell membrane {ECO:0000250}. Note=Colocalizes
CC       with AP-2 at the plasma membrane. {ECO:0000250}.
CC   -!- DOMAIN: The WXXF motifs mediate binding of accessory proteins to the
CC       ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions.
CC       Selective binding to the GAE domains of AP-1 or to the alpha-ear domain
CC       of AP-2 is tuned by the acidic context surrounding the motif and the
CC       properties of the second residue of the motif itself (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NECAP family. {ECO:0000305}.
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DR   EMBL; BT020866; AAX08883.1; -; mRNA.
DR   EMBL; BC109915; AAI09916.1; -; mRNA.
DR   RefSeq; NP_001015562.1; NM_001015562.1.
DR   AlphaFoldDB; Q5E9Q4; -.
DR   SMR; Q5E9Q4; -.
DR   STRING; 9913.ENSBTAP00000017663; -.
DR   PaxDb; Q5E9Q4; -.
DR   PRIDE; Q5E9Q4; -.
DR   GeneID; 509439; -.
DR   KEGG; bta:509439; -.
DR   CTD; 55707; -.
DR   eggNOG; KOG2500; Eukaryota.
DR   HOGENOM; CLU_069884_1_0_1; -.
DR   InParanoid; Q5E9Q4; -.
DR   OrthoDB; 1605812at2759; -.
DR   TreeFam; TF314482; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0030125; C:clathrin vesicle coat; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR   CDD; cd13228; PHear_NECAP; 1.
DR   Gene3D; 2.30.29.30; -; 1.
DR   InterPro; IPR012466; NECAP_PHear.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   Pfam; PF07933; DUF1681; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Cytoplasmic vesicle; Endocytosis; Membrane; Phosphoprotein;
KW   Protein transport; Reference proteome; Repeat; Transport.
FT   CHAIN           1..266
FT                   /note="Adaptin ear-binding coat-associated protein 2"
FT                   /id="PRO_0000283723"
FT   REGION          164..191
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          244..266
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           243..246
FT                   /note="WXXF motif 1"
FT   MOTIF           263..266
FT                   /note="WXXF motif 2"
FT   MOD_RES         181
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NVZ3"
SQ   SEQUENCE   266 AA;  28551 MW;  976657105866B1D1 CRC64;
     MEEAEYESVL CVKPDVHVYR IPPRATNRGY RAAEWQLDQP SWSGRLRITA KGQVAYIKLE
     DRTSGELFAQ APVDQFPGTA VESVTDSSRY FVIRIEDGNG RRAFIGIGFG DRGDAFDFNV
     ALQDHFKWVK QQCEFAKQAQ NPDQGPKLDL SFKEGQTIKL NIASMKKKDG AAGTPRARPT
     STGGLSLLPP PPGAKTAALA PLSGEHLSVG GSVVQPAVSP SSGGATVSWP QPKPATTATA
     DIWGDFTKST GSTSSQTQPG AGWVQF