NECP2_BOVIN
ID NECP2_BOVIN Reviewed; 266 AA.
AC Q5E9Q4;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Adaptin ear-binding coat-associated protein 2;
DE AltName: Full=NECAP endocytosis-associated protein 2;
DE Short=NECAP-2;
GN Name=NECAP2;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT "Characterization of 954 bovine full-CDS cDNA sequences.";
RL BMC Genomics 6:166-166(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Liver;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in endocytosis. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with AP1G1 and AP2A1 components of the adapter
CC protein complexes AP-1 and AP-2. Interacts with the GAE domain proteins
CC GGA1, GGA2 and GGA3 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, clathrin-coated vesicle
CC membrane {ECO:0000250}. Cell membrane {ECO:0000250}. Note=Colocalizes
CC with AP-2 at the plasma membrane. {ECO:0000250}.
CC -!- DOMAIN: The WXXF motifs mediate binding of accessory proteins to the
CC ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions.
CC Selective binding to the GAE domains of AP-1 or to the alpha-ear domain
CC of AP-2 is tuned by the acidic context surrounding the motif and the
CC properties of the second residue of the motif itself (By similarity).
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the NECAP family. {ECO:0000305}.
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DR EMBL; BT020866; AAX08883.1; -; mRNA.
DR EMBL; BC109915; AAI09916.1; -; mRNA.
DR RefSeq; NP_001015562.1; NM_001015562.1.
DR AlphaFoldDB; Q5E9Q4; -.
DR SMR; Q5E9Q4; -.
DR STRING; 9913.ENSBTAP00000017663; -.
DR PaxDb; Q5E9Q4; -.
DR PRIDE; Q5E9Q4; -.
DR GeneID; 509439; -.
DR KEGG; bta:509439; -.
DR CTD; 55707; -.
DR eggNOG; KOG2500; Eukaryota.
DR HOGENOM; CLU_069884_1_0_1; -.
DR InParanoid; Q5E9Q4; -.
DR OrthoDB; 1605812at2759; -.
DR TreeFam; TF314482; -.
DR Proteomes; UP000009136; Unplaced.
DR GO; GO:0030125; C:clathrin vesicle coat; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR CDD; cd13228; PHear_NECAP; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR012466; NECAP_PHear.
DR InterPro; IPR011993; PH-like_dom_sf.
DR Pfam; PF07933; DUF1681; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Cytoplasmic vesicle; Endocytosis; Membrane; Phosphoprotein;
KW Protein transport; Reference proteome; Repeat; Transport.
FT CHAIN 1..266
FT /note="Adaptin ear-binding coat-associated protein 2"
FT /id="PRO_0000283723"
FT REGION 164..191
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 244..266
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 243..246
FT /note="WXXF motif 1"
FT MOTIF 263..266
FT /note="WXXF motif 2"
FT MOD_RES 181
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NVZ3"
SQ SEQUENCE 266 AA; 28551 MW; 976657105866B1D1 CRC64;
MEEAEYESVL CVKPDVHVYR IPPRATNRGY RAAEWQLDQP SWSGRLRITA KGQVAYIKLE
DRTSGELFAQ APVDQFPGTA VESVTDSSRY FVIRIEDGNG RRAFIGIGFG DRGDAFDFNV
ALQDHFKWVK QQCEFAKQAQ NPDQGPKLDL SFKEGQTIKL NIASMKKKDG AAGTPRARPT
STGGLSLLPP PPGAKTAALA PLSGEHLSVG GSVVQPAVSP SSGGATVSWP QPKPATTATA
DIWGDFTKST GSTSSQTQPG AGWVQF