NECP2_RAT
ID NECP2_RAT Reviewed; 263 AA.
AC Q6P756;
DT 29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 29-MAR-2005, sequence version 2.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Adaptin ear-binding coat-associated protein 2;
DE Short=NECAP-2;
GN Name=Necap2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Prostate;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-182 (ISOFORM 1).
RX PubMed=14766980; DOI=10.1073/pnas.0305170101;
RA Hong S.J., Li H., Becker K.G., Dawson V.L., Dawson T.M.;
RT "Identification and analysis of plasticity-induced late-response genes.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:2145-2150(2004).
RN [3]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=14555962; DOI=10.1038/sj.embor.embor7400004;
RA Ritter B., Philie J., Girard M., Tung E.C., Blondeau F., McPherson P.S.;
RT "Identification of a family of endocytic proteins that define a new alpha-
RT adaptin ear-binding motif.";
RL EMBO Rep. 4:1089-1095(2003).
CC -!- FUNCTION: Involved in endocytosis. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with AP1G1 and AP2A1 components of the adapter
CC protein complexes AP-1 and AP-2. Interacts with the GAE domain proteins
CC GGA1, GGA2 and GGA3 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, clathrin-coated vesicle
CC membrane {ECO:0000269|PubMed:14555962}. Cell membrane
CC {ECO:0000269|PubMed:14555962}. Note=Partially colocalizes with AP-2 at
CC the plasma membrane. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6P756-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6P756-2; Sequence=VSP_013237;
CC -!- TISSUE SPECIFICITY: Expressed in brain, heart, kidney, liver and lung
CC (at protein level). {ECO:0000269|PubMed:14555962}.
CC -!- DOMAIN: The WXXF motifs mediate binding of accessory proteins to the
CC ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions.
CC Selective binding to the GAE domains of AP-1 or to the alpha-ear domain
CC of AP-2 is tuned by the acidic context surrounding the motif and the
CC properties of the second residue of the motif itself (By similarity).
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the NECAP family. {ECO:0000305}.
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DR EMBL; BC061826; AAH61826.1; -; mRNA.
DR EMBL; CK366762; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; CB724981; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_954527.1; NM_199096.1.
DR RefSeq; XP_006239241.1; XM_006239179.3. [Q6P756-1]
DR AlphaFoldDB; Q6P756; -.
DR SMR; Q6P756; -.
DR BioGRID; 255956; 5.
DR STRING; 10116.ENSRNOP00000011394; -.
DR iPTMnet; Q6P756; -.
DR PhosphoSitePlus; Q6P756; -.
DR PaxDb; Q6P756; -.
DR PRIDE; Q6P756; -.
DR Ensembl; ENSRNOT00000100304; ENSRNOP00000095787; ENSRNOG00000008427. [Q6P756-1]
DR GeneID; 298598; -.
DR KEGG; rno:298598; -.
DR UCSC; RGD:735063; rat. [Q6P756-1]
DR CTD; 55707; -.
DR RGD; 735063; Necap2.
DR eggNOG; KOG2500; Eukaryota.
DR GeneTree; ENSGT00390000009359; -.
DR HOGENOM; CLU_069884_1_0_1; -.
DR InParanoid; Q6P756; -.
DR OMA; NEGHRAQ; -.
DR OrthoDB; 1605812at2759; -.
DR PhylomeDB; Q6P756; -.
DR TreeFam; TF314482; -.
DR Reactome; R-RNO-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR Reactome; R-RNO-8856828; Clathrin-mediated endocytosis.
DR PRO; PR:Q6P756; -.
DR Proteomes; UP000002494; Chromosome 5.
DR Bgee; ENSRNOG00000008427; Expressed in thymus and 20 other tissues.
DR Genevisible; Q6P756; RN.
DR GO; GO:0030125; C:clathrin vesicle coat; ISS:UniProtKB.
DR GO; GO:0005905; C:clathrin-coated pit; ISS:UniProtKB.
DR GO; GO:0006897; P:endocytosis; ISS:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR CDD; cd13228; PHear_NECAP; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR012466; NECAP_PHear.
DR InterPro; IPR011993; PH-like_dom_sf.
DR Pfam; PF07933; DUF1681; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Cytoplasmic vesicle; Endocytosis;
KW Membrane; Phosphoprotein; Protein transport; Reference proteome; Repeat;
KW Transport.
FT CHAIN 1..263
FT /note="Adaptin ear-binding coat-associated protein 2"
FT /id="PRO_0000213073"
FT REGION 167..191
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 209..263
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 218..221
FT /note="WXXF motif 1"
FT MOTIF 238..241
FT /note="WXXF motif 2"
FT COMPBIAS 209..224
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 242..263
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 181
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NVZ3"
FT VAR_SEQ 1..70
FT /note="MEESEYESVLCVKPEVHVYRIPPRATNRGYRASEWQLDQPSWSGRLRITAKG
FT KVAYIKLEDRTSGELFAQ -> MSTASRRGPPTVVTGPQNGNWTSHPGVAGCGSLRKGR
FT WPTSSWRTGPL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_013237"
SQ SEQUENCE 263 AA; 28405 MW; D9210ED9003CCDD1 CRC64;
MEESEYESVL CVKPEVHVYR IPPRATNRGY RASEWQLDQP SWSGRLRITA KGKVAYIKLE
DRTSGELFAQ APVDQFPGTA VESVTDSSRY FVIRIEDGNG RRAFIGIGFG DRGDAFDFNV
ALQDHFKWVK QQCEFAKQAQ NPDEGPKLDL GFKEGQTIKI NIANMRKKEG AAGAPRTRPA
SAGGLSLLPP PPGGKMSTLI PPSGEQFSGG SLVQPVSGSG GATELWPQSK PAAAATADIW
GDFTKSTGSP SSQSQPGTGW VQF
