NEED5_BPT4
ID NEED5_BPT4 Reviewed; 97 AA.
AC P39234;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 64.
DE RecName: Full=Baseplate puncturing device gp5.4 {ECO:0000303|PubMed:27193680};
DE AltName: Full=Gene product 5.4;
DE Short=gp5.4;
GN Name=5.4; Synonyms=y08B;
OS Enterobacteria phage T4 (Bacteriophage T4).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX NCBI_TaxID=10665;
OH NCBI_TaxID=562; Escherichia coli.
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT "Bacteriophage T4 genome.";
RL Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN [2] {ECO:0007744|PDB:4KU0}
RP X-RAY CRYSTALLOGRAPHY (1.15 ANGSTROMS) OF 2-97 IN COMPLEX WITH IRON,
RP COFACTOR, AND FUNCTION.
RA Buth S.A., Leiman P.G., Shneider M.M.;
RT "Crystall structute of the business end of the T4 cell-puncturing device.";
RL Submitted (MAY-2013) to the PDB data bank.
RN [3] {ECO:0007744|PDB:5IV5}
RP STRUCTURE BY ELECTRON MICROSCOPY (4.11 ANGSTROMS), SUBUNIT, SUBCELLULAR
RP LOCATION, FUNCTION, INTERACTION WITH GP27, AND INTERACTION WITH GP5.
RX PubMed=27193680; DOI=10.1038/nature17971;
RA Taylor N.M., Prokhorov N.S., Guerrero-Ferreira R.C., Shneider M.M.,
RA Browning C., Goldie K.N., Stahlberg H., Leiman P.G.;
RT "Structure of the T4 baseplate and its function in triggering sheath
RT contraction.";
RL Nature 533:346-352(2016).
CC -!- FUNCTION: Baseplate central spike complex-associated protein. Makes the
CC tip of the central spike which probably punctures the host outer
CC membrane, a necessary step for viral DNA ejection into the host cell.
CC {ECO:0000305|PubMed:27193680, ECO:0000305|Ref.2}.
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000269|Ref.2};
CC -!- SUBUNIT: Heteromultimer with gp27 trimer and gp5 trimer; this
CC interaction forms the tail lysozyme complex that creates an extension
CC of the tail tube (PubMed:27193680). Part of the baseplate
CC macromolecular complex which consists of gp5, gp5.4, gp27 (central
CC spike complex); gp6, gp25, gp53 (inner baseplate); gp7, gp8
CC (intermediate baseplate); gp9, gp10, gp11, gp12 (peripheral); gp48 and
CC gp54 (proximal region of the tail tube). {ECO:0000269|PubMed:27193680}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:27193680}.
CC Note=Present in the baseplate. {ECO:0000269|PubMed:27193680}.
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DR EMBL; AF158101; AAD42485.1; -; Genomic_DNA.
DR RefSeq; NP_049763.1; NC_000866.4.
DR PDB; 4KU0; X-ray; 1.15 A; D=2-97.
DR PDB; 5IV5; EM; 4.11 A; ZA=1-97.
DR PDBsum; 4KU0; -.
DR PDBsum; 5IV5; -.
DR SMR; P39234; -.
DR GeneID; 1258818; -.
DR KEGG; vg:1258818; -.
DR Proteomes; UP000009087; Genome.
DR GO; GO:0098025; C:virus tail, baseplate; IDA:UniProtKB.
DR GO; GO:0003796; F:lysozyme activity; IDA:CACAO.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0033922; F:peptidoglycan beta-N-acetylmuramidase activity; IDA:CACAO.
DR InterPro; IPR008727; PAAR_motif.
DR Pfam; PF05488; PAAR_motif; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Iron; Metal-binding; Reference proteome; Virion.
FT CHAIN 1..97
FT /note="Baseplate puncturing device gp5.4"
FT /id="PRO_0000165155"
FT BINDING 14
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0007744|PDB:4KU0"
FT BINDING 46
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0007744|PDB:4KU0"
FT BINDING 56
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0007744|PDB:4KU0"
FT BINDING 83
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0007744|PDB:4KU0"
FT STRAND 28..31
FT /evidence="ECO:0007829|PDB:4KU0"
FT STRAND 34..36
FT /evidence="ECO:0007829|PDB:4KU0"
FT STRAND 65..69
FT /evidence="ECO:0007829|PDB:4KU0"
FT STRAND 72..74
FT /evidence="ECO:0007829|PDB:4KU0"
FT STRAND 92..96
FT /evidence="ECO:0007829|PDB:4KU0"
SQ SEQUENCE 97 AA; 10221 MW; D7C63CD65737C824 CRC64;
MSGLSYDKCV TAGHEAWPPT VVNATQSKVF TGGIAVLVAG DPITEHTEIK KPYETHGGVT
QPRTSKVYVT GKKAVQMADP ISCGDTVAQA SSKVFIK
