NEEDL_BPP22
ID NEEDL_BPP22 Reviewed; 233 AA.
AC P35837; I7KJL6; Q8LTG5;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 02-JUN-2021, entry version 91.
DE RecName: Full=Tail needle protein gp26;
DE AltName: Full=Head completion protein;
DE AltName: Full=Packaged DNA stabilization protein;
DE AltName: Full=Tail accessory factor gp26;
GN Name=26;
OS Salmonella phage P22 (Bacteriophage P22).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Podoviridae; Lederbergvirus.
OX NCBI_TaxID=10754;
OH NCBI_TaxID=90371; Salmonella typhimurium.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8341611; DOI=10.1093/nar/21.14.3326;
RA Casjens S.R., Sampson L.;
RT "Nucleotide sequence of Salmonella bacteriophage P22 head completion genes
RT 10 and 26.";
RL Nucleic Acids Res. 21:3326-3326(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11053393; DOI=10.1128/jb.182.22.6472-6481.2000;
RA Vander Byl C.S., Kropinski A.M.B.;
RT "Sequence of the genome of Salmonella bacteriophage P22.";
RL J. Bacteriol. 182:6472-6481(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12562822; DOI=10.1128/jb.185.4.1475-1477.2003;
RA Pedulla M.L., Ford M.E., Karthikeyan T., Houtz J.M., Hendrix R.W.,
RA Hatfull G.F., Poteete A.R., Gilcrease E.B., Winn-Stapley D.A.,
RA Casjens S.R.;
RT "Corrected sequence of the bacteriophage P22 genome.";
RL J. Bacteriol. 185:1475-1477(2003).
RN [4]
RP FUNCTION.
RX PubMed=20817910; DOI=10.1074/jbc.m110.169003;
RA Andres D., Hanke C., Baxa U., Seul A., Barbirz S., Seckler R.;
RT "Tailspike interactions with lipopolysaccharide effect DNA ejection from
RT phage P22 particles in vitro.";
RL J. Biol. Chem. 285:36768-36775(2010).
RN [5]
RP FUNCTION.
RX PubMed=30886360; DOI=10.1038/s41564-019-0403-z;
RA Wang C., Tu J., Liu J., Molineux I.J.;
RT "Structural dynamics of bacteriophage P22 infection initiation revealed by
RT cryo-electron tomography.";
RL Nat. Microbiol. 4:1049-1056(2019).
RN [6]
RP ELECTRON MICROSCOPY (20 ANGSTROMS) OF COMPLETE VIRION, AND SUBUNIT.
RX PubMed=16730179; DOI=10.1016/j.str.2006.05.007;
RA Chang J., Weigele P., King J., Chiu W., Jiang W.;
RT "Cryo-EM asymmetric reconstruction of bacteriophage P22 reveals
RT organization of its DNA packaging and infecting machinery.";
RL Structure 14:1073-1082(2006).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS), SUBUNIT, AND FUNCTION.
RX PubMed=18059287; DOI=10.1038/nsmb1317;
RA Olia A.S., Casjens S., Cingolani G.;
RT "Structure of phage P22 cell envelope-penetrating needle.";
RL Nat. Struct. Mol. Biol. 14:1221-1226(2007).
CC -!- FUNCTION: Cell-perforating component and plug protein of the phage tail
CC machine (PubMed:18059287, PubMed:20817910). Together with gp4 and gp10,
CC gp26 is required for stabilization of the condensed DNA within the
CC capsid by plugging the hole through which the DNA enters
CC (PubMed:18059287). Host cell membrane perforation allows viral DNA
CC ejection (PubMed:18059287). The needle penetrates the host outer
CC membrane. The needle is released and the internal head protein gp7 is
CC ejected to form an extra-cellular channel (PubMed:30886360).
CC {ECO:0000269|PubMed:18059287, ECO:0000269|PubMed:20817910,
CC ECO:0000269|PubMed:30886360}.
CC -!- SUBUNIT: Elongated coiled-coil homotrimer (240A x 25A). Interacts with
CC gp10. {ECO:0000269|PubMed:16730179, ECO:0000269|PubMed:18059287}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}.
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DR EMBL; L14810; AAA32271.1; -; Genomic_DNA.
DR EMBL; AF217253; AAF75051.1; -; Genomic_DNA.
DR EMBL; AF527608; AAM81393.1; -; Genomic_DNA.
DR EMBL; BK000583; DAA00991.1; -; Genomic_DNA.
DR PIR; S34956; S34956.
DR RefSeq; YP_063715.1; NC_002371.2.
DR PDB; 2POH; X-ray; 2.10 A; A/B/C/D/E/F=1-233.
DR PDB; 3C9I; X-ray; 1.95 A; A/B/C/D/E/F=1-233.
DR PDB; 4LIN; X-ray; 2.70 A; A/B/C/D/E/F/G/H/I/J/K/L=1-233.
DR PDB; 4ZKP; X-ray; 2.10 A; A/B=1-233.
DR PDB; 4ZKU; X-ray; 2.50 A; A/B=1-233.
DR PDB; 4ZXQ; X-ray; 2.75 A; A/B/C/D/E/F=1-140.
DR PDBsum; 2POH; -.
DR PDBsum; 3C9I; -.
DR PDBsum; 4LIN; -.
DR PDBsum; 4ZKP; -.
DR PDBsum; 4ZKU; -.
DR PDBsum; 4ZXQ; -.
DR SMR; P35837; -.
DR DIP; DIP-60502N; -.
DR TCDB; 1.K.4.1.1; the phage p22 injectisome (p22 injectisome) family.
DR GeneID; 2944243; -.
DR KEGG; vg:2944243; -.
DR EvolutionaryTrace; P35837; -.
DR Proteomes; UP000001795; Genome.
DR Proteomes; UP000001796; Genome.
DR Proteomes; UP000007960; Genome.
DR GO; GO:0098015; C:virus tail; IEA:UniProtKB-KW.
DR GO; GO:0044694; P:pore-mediated entry of viral genome into host cell; IEA:UniProtKB-KW.
DR GO; GO:0099002; P:viral genome ejection through host cell envelope, short tail mechanism; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW 3D-structure; Coiled coil; Late protein;
KW Pore-mediated penetration of viral genome into host cell;
KW Reference proteome; Repeat;
KW Viral genome ejection through host cell envelope;
KW Viral penetration into host cytoplasm; Viral short tail ejection system;
KW Viral tail protein; Virion; Virus entry into host cell.
FT INIT_MET 1
FT /note="Removed; by host"
FT /evidence="ECO:0000305"
FT CHAIN 2..233
FT /note="Tail needle protein gp26"
FT /id="PRO_0000077767"
FT REPEAT 77..84
FT /note="Trimerization octad repeat 1"
FT REPEAT 98..105
FT /note="Trimerization octad repeat 2"
FT REPEAT 112..119
FT /note="Trimerization octad repeat 3"
FT REPEAT 133..140
FT /note="Trimerization octad repeat 4"
FT REPEAT 205..212
FT /note="Trimerization octad repeat 5"
FT REGION 2..27
FT /note="Interaction with gp10"
FT REGION 28..140
FT /note="Fibrous core"
FT REGION 141..170
FT /note="Beta-helix"
FT REGION 171..233
FT /note="Lazo"
FT MOTIF 216..220
FT /note="Basic cluster"
FT SITE 200
FT /note="Needle distal tip"
FT CONFLICT 169
FT /note="L -> V (in Ref. 3; AAM81393/DAA00991)"
FT /evidence="ECO:0000305"
FT HELIX 4..7
FT /evidence="ECO:0007829|PDB:3C9I"
FT STRAND 19..22
FT /evidence="ECO:0007829|PDB:3C9I"
FT TURN 24..26
FT /evidence="ECO:0007829|PDB:3C9I"
FT HELIX 29..139
FT /evidence="ECO:0007829|PDB:3C9I"
FT STRAND 142..144
FT /evidence="ECO:0007829|PDB:3C9I"
FT STRAND 155..159
FT /evidence="ECO:0007829|PDB:3C9I"
FT STRAND 161..163
FT /evidence="ECO:0007829|PDB:3C9I"
FT STRAND 166..169
FT /evidence="ECO:0007829|PDB:3C9I"
FT STRAND 179..181
FT /evidence="ECO:0007829|PDB:4LIN"
FT HELIX 202..229
FT /evidence="ECO:0007829|PDB:3C9I"
FT STRAND 230..232
FT /evidence="ECO:0007829|PDB:2POH"
SQ SEQUENCE 233 AA; 24734 MW; B4564B274B5564D3 CRC64;
MADPSLNNPV VIQATRLDAS ILPRNVFSKS YLLYVIAQGT DVGAIAGKAN EAGQGAYDAQ
VKNDEQDVEL ADHEARIKQL RIDVDDHESR ITANTKAITA LNVRVTTAEG EIASLQTNVS
ALDGRVTTAE NNISALQADY VSKTATTSQS LASPLNVTTS YSVGGKKVLG ARQTGWTAAT
GTANKGVFDA DLTFAVSDTY TQSEIQAIAN ALITERRRTK ALEDALRAHG LID
