ID   NEED_PINRA              Reviewed;         404 AA.
AC   O04407;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Floricaula/leafy-like protein FL1;
DE   AltName: Full=Protein NEEDLY;
OS   Pinus radiata (Monterey pine) (Pinus insignis).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Pinus;
OC   Pinus subgen. Pinus.
OX   NCBI_TaxID=3347;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Cone;
RA   Mouradov A., Glassick T., Teasdale R.D.;
RT   "Isolation and characterisation of a FLORICAULA/LEAFY-like cDNA from Pinus
RT   radiata.";
RL   (er) Plant Gene Register PGR97-026(1997).
RN   [2]
RP   CHARACTERIZATION.
RX   PubMed=9601002; DOI=10.1073/pnas.95.11.6537;
RA   Mouradov A., Glassick T., Hamdorf B., Murphy L., Fowler B., Marla S.,
RA   Teasdale R.D.;
RT   "NEEDLY, a Pinus radiata ortholog of FLORICAULA/LEAFY genes, expressed in
RT   both reproductive and vegetative meristems.";
RL   Proc. Natl. Acad. Sci. U.S.A. 95:6537-6542(1998).
CC   -!- FUNCTION: Probable transcription factor. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in both male and female cones, vegetative
CC       buds and needles, but not in the roots.
CC   -!- SIMILARITY: Belongs to the FLO/LFY family. {ECO:0000305}.
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DR   EMBL; U76757; AAB68601.1; -; mRNA.
DR   PIR; T09606; T09606.
DR   AlphaFoldDB; O04407; -.
DR   SMR; O04407; -.
DR   PRIDE; O04407; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.4180.10; -; 1.
DR   InterPro; IPR035209; FLO/LFY_C.
DR   InterPro; IPR002910; FLO_LFY.
DR   InterPro; IPR038276; Floricaula/leafy_C_sf.
DR   InterPro; IPR035079; LFY_SAM.
DR   PANTHER; PTHR36079; PTHR36079; 1.
DR   Pfam; PF17538; C_LFY_FLO; 1.
DR   Pfam; PF01698; SAM_LFY; 1.
PE   1: Evidence at protein level;
KW   Activator; Developmental protein; DNA-binding; Nucleus; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..404
FT                   /note="Floricaula/leafy-like protein FL1"
FT                   /id="PRO_0000129154"
FT   DNA_BIND        246..250
FT                   /evidence="ECO:0000250"
FT   DNA_BIND        315..322
FT                   /evidence="ECO:0000250"
FT   DNA_BIND        386..389
FT                   /evidence="ECO:0000250"
FT   REGION          210..251
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        215..251
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            293
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000250"
FT   SITE            300
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000250"
FT   SITE            304
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000250"
FT   SITE            351
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   404 AA;  46487 MW;  1255D124C021CF35 CRC64;
     MDAEHFPVGF FRWDQRPAPV VAAAAAPTTT VFNKDHGRPL EVILPMNGRK DLKSLEDLFK
     EYGVRYVTLA KMTEMGFTAN TLVNMTEEEI EDLMKTLVEL YHMDLLIGER YGIKSAIRAE
     KKRLQDSLEM QRLEILSEAE RKRILHDDQN TFAAAMASEG TSKELRANDP LIFPESTSAD
     HAPMNIASCK DSTLILQNSN QAQFCGSGLI GVPEHSSESD ERKADTNKQK RRRSKEPGED
     GEDRPREHPF IVTEPGELAR GKKNGLDYLF DLYEQCGKFL LEVQRIAKEK GEKCPTKVTN
     QVFRHAKHNG AVYINKPKMR HYVHCYALHC LDSEQSNHLR RLYKERGENV GAWRQACYYP
     LVAIARENNW DIEGIFNRNE KLKIWYVPTK LRQLCHMERS KECQ