ID   NEF_SIVG1               Reviewed;         223 AA.
AC   Q02840;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Protein Nef;
DE   AltName: Full=3'ORF;
DE   AltName: Full=Negative factor;
DE            Short=F-protein;
GN   Name=nef;
OS   Simian immunodeficiency virus agm.grivet (isolate AGM gr-1) (SIV-agm.gri)
OS   (Simian immunodeficiency virus African green monkey grivet).
OC   Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes;
OC   Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus.
OX   NCBI_TaxID=31684;
OH   NCBI_TaxID=9527; Cercopithecidae (Old World monkeys).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2024476; DOI=10.1016/0042-6822(91)90689-9;
RA   Fomsgaard A., Hirsch V.M., Allan J.S., Johnson P.R.;
RT   "A highly divergent proviral DNA clone of SIV from a distinct species of
RT   African green monkey.";
RL   Virology 182:397-402(1991).
CC   -!- FUNCTION: Seems to play a role in optimizing the host cell environment
CC       for viral replication without causing cell death by apoptosis. Enhances
CC       virus infectivity and pathogenicity. Probably involved in viral immune
CC       evasion mechanisms (By similarity). {ECO:0000250}.
CC   -!- FUNCTION: In infected CD4(+) T-lymphocytes, down-regulates cell surface
CC       expression of CD4, CD28, CD3, and MHC-I or MHC-II molecules.
CC       {ECO:0000250}.
CC   -!- FUNCTION: Interferes with TCR signaling from the cell membrane.
CC       Interacts with CD247/TCRZ (TCR zeta chain) and exert potent down-
CC       regulation of cell surface TCR/CD3 complexes (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. Interacts with host CD247/TCRZ; this interaction
CC       induces down-regulation of cell surface TCR/CD3 complexes.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cell membrane {ECO:0000250}; Lipid-anchor
CC       {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Note=Associates with the
CC       inner plasma membrane through its N-terminal domain. {ECO:0000250}.
CC   -!- DOMAIN: The N-terminal domain is composed of the N-myristoyl glycine
CC       and of a cluster of positively charged amino acids. It is required for
CC       inner plasma membrane targeting of Nef (By similarity). {ECO:0000250}.
CC   -!- MISCELLANEOUS: This is an African green monkey isolate.
CC   -!- SIMILARITY: Belongs to the lentivirus primate group Nef protein family.
CC       {ECO:0000305}.
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DR   EMBL; M66437; AAA91929.1; -; Genomic_DNA.
DR   EMBL; M58410; AAA47592.1; -; Genomic_RNA.
DR   RefSeq; NP_054373.1; NC_001549.1.
DR   SMR; Q02840; -.
DR   BioGRID; 3509205; 5.
DR   IntAct; Q02840; 3.
DR   GeneID; 1490008; -.
DR   KEGG; vg:1490008; -.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005525; F:GTP binding; IEA:InterPro.
DR   Gene3D; 3.30.62.10; -; 1.
DR   InterPro; IPR027481; HIV-1_Nef_core_sf.
DR   InterPro; IPR001558; HIV_Nef.
DR   Pfam; PF00469; F-protein; 1.
DR   SUPFAM; SSF55671; SSF55671; 1.
PE   3: Inferred from homology;
KW   Host cell membrane; Host membrane; Host-virus interaction; Lipoprotein;
KW   Membrane; Myristate; Viral immunoevasion; Virulence.
FT   INIT_MET        1
FT                   /note="Removed; by host"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..223
FT                   /note="Protein Nef"
FT                   /id="PRO_0000085241"
FT   REGION          73..81
FT                   /note="Acidic"
FT   REGION          124..140
FT                   /note="Mediates dimerization"
FT                   /evidence="ECO:0000250"
FT   LIPID           2
FT                   /note="N-myristoyl glycine; by host"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   223 AA;  25695 MW;  80E7B0175C5923FD CRC64;
     MGSSNSKRQQ QGLLKLWRGL RGKPGADWVL LSDPLIGQSS TVQEECGKAL KKSWGKGKMT
     PDGRRLQEGD TFDEWDDDEE EVGFPVQPRV PLRQMTYKLA VDFSHFLKSK GGLDGIYYSE
     RREKILNLYA LNEWGIIDDW QAYSPGPGIR YPRVFGFCFK LVPVDLHEEA RNCERHCLMH
     PAQMGEDPDG IDHGEVLVWK FDPKLAVEYR PDMFKDMHEH AKR