NEK6_ARATH
ID NEK6_ARATH Reviewed; 416 AA.
AC Q9LT35; F4JEU3;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Serine/threonine-protein kinase Nek6;
DE EC=2.7.11.1;
DE AltName: Full=NimA-related protein kinase 6;
DE Short=AtNek6;
GN Name=NEK6; OrderedLocusNames=At3g20860; ORFNames=MOE17.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17886359; DOI=10.1111/j.1365-313x.2007.03161.x;
RA Vigneault F., Lachance D., Cloutier M., Pelletier G., Levasseur C.,
RA Seguin A.;
RT "Members of the plant NIMA-related kinases are involved in organ
RT development and vascularization in poplar, Arabidopsis and rice.";
RL Plant J. 51:575-588(2007).
CC -!- FUNCTION: May be involved in plant development processes.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. NEK Ser/Thr
CC protein kinase family. NIMA subfamily. {ECO:0000305}.
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DR EMBL; AB025629; BAB02494.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76432.2; -; Genomic_DNA.
DR RefSeq; NP_188722.2; NM_112977.2.
DR AlphaFoldDB; Q9LT35; -.
DR SMR; Q9LT35; -.
DR BioGRID; 6966; 1.
DR STRING; 3702.AT3G20860.1; -.
DR PaxDb; Q9LT35; -.
DR PRIDE; Q9LT35; -.
DR ProteomicsDB; 238238; -.
DR EnsemblPlants; AT3G20860.1; AT3G20860.1; AT3G20860.
DR GeneID; 821634; -.
DR Gramene; AT3G20860.1; AT3G20860.1; AT3G20860.
DR KEGG; ath:AT3G20860; -.
DR Araport; AT3G20860; -.
DR eggNOG; KOG0589; Eukaryota.
DR HOGENOM; CLU_000288_128_3_1; -.
DR InParanoid; Q9LT35; -.
DR OMA; TPIKHNQ; -.
DR OrthoDB; 70360at2759; -.
DR PhylomeDB; Q9LT35; -.
DR PRO; PR:Q9LT35; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LT35; baseline and differential.
DR Genevisible; Q9LT35; AT.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0043622; P:cortical microtubule organization; IMP:CACAO.
DR GO; GO:0010311; P:lateral root formation; IMP:CACAO.
DR GO; GO:0010366; P:negative regulation of ethylene biosynthetic process; IMP:CACAO.
DR GO; GO:0006468; P:protein phosphorylation; IDA:CACAO.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 3: Inferred from homology;
KW ATP-binding; Kinase; Nucleotide-binding; Reference proteome;
KW Serine/threonine-protein kinase; Transferase.
FT CHAIN 1..416
FT /note="Serine/threonine-protein kinase Nek6"
FT /id="PRO_0000314042"
FT DOMAIN 4..257
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 277..334
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 277..292
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 298..329
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 128
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 10..18
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 33
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
SQ SEQUENCE 416 AA; 47251 MW; 917AA7B9000A3088 CRC64;
MDDYEVVEQI GRGAFGSAFL VIHKSERRKY VVKKIRLAKQ TERCKLAAIQ EMSLISKLKS
PYIVEYKDSW VEKDCVCIVT SYCEGGDMTQ MIKKSRGVFA SEEKLCRWMV QLLLAIDYLH
NNRVLHRDLK CSNIFLTKEN EVRLGDFGLA KLLGKDDLAS SMVGTPNYMC PELLADIPYG
YKSDIWSLGC CMFEVAAHQP AFKAPDMAAL INKINRSSLS PLPVMYSSSL KRLIKSMLRK
NPEHRPTAAE LLRHPHLQPY LAQCQNLSPV FKPVVSKSEH NTNENRTGLP PKTKSAKTPI
KHNQESEETE KKNKDTSSSS KDKERPAKSQ EMSVISTLTL LREFQKKSPK SEERAEALES
LLELCAGLLR QEKFDELEGV LKPFGDETVS SRETAIWLTK SLMNVKRKQN DDETNI
